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Fluorescence Correlation Spectroscopy (FCS) is a powerful single-molecule technique which allows for measuring motion (diffusion, flow), concentration, and molecular interaction kinetics of fluorescent molecules from picomolar to micromolar…

The performance of a confocal imaging system may be no better than a general-purpose widefield system if it is not properly maintained or quality controlled. The publication of ISO 21073, 'Confocal microscopes- Optical data of fluorescence…

Other Quantitative Biology · Quantitative Biology 2020-11-20 Glyn Nelson , Laurent Gelman , Orestis Faklaris , Roland Nitschke , Alex Laude

Real time, singleshot multispectral imaging systems are crucial for environment monitoring and biomedical imaging. Most singleshot multispectral imagers rely on complex computational backends, which precludes real time operations. In this…

Optics · Physics 2025-02-27 Romil Audhkhasi , Ningzhi Xie , Johannes E. Fröch , Arka Majumdar

Optical coherence tomography (OCT) microscope (OCM) uses a high-numerical-aperture objective to achieve cellular-level lateral resolution. However, its practical imaging depth range is limited by the depth of focus (DOF). Although…

Traditional glass-based optics are typically optimized for narrow spectral bands, such as the visible (400-700nm) or shortwave infrared (1000-1800nm). While the emergence of VIS-SWIR sensors (400-1700nm) offers transformative potential,…

Image and Video Processing · Electrical Eng. & Systems 2026-05-04 Vishwanath Saragadam , Niki Nezakati , Amit Roy-Chowdhury , Vivek Boominathan

Quantum imaging is an advanced method for microscopy or investigating the optical properties of materials or bio-medical inspections with high accuracy, low noise, and extremely low photo-damage. In previous work, we proposed a quantum…

Optics · Physics 2025-05-09 Mousume Samad , Maki Shimizu , Yasuto Hijikata

In chiral sum frequency generation (C-SFG), the chiral nature of ${\chi}^{(2)}$ requires the three involved electric fields to be pairwise non-parallel, leading to the traditional non-collinear configuration which is a hindrance for…

Optics · Physics 2016-12-07 Ziheng Ji , Wentao Yu , Hong Yang , Kaihui Liu , Qihuang Gong , Zhiwen Liu , Kebin Shi

Although several optical techniques have been recently developed in order to overcome the resolution limit in microscopy, the imaging of sub-wavelength features is still a real challenge. In practise, super-resolution techniques remain…

Physics Education · Physics 2019-07-24 Stephane Perrin , Keshia Badu , Paul Montgomery , Sylvain Lecler

We present a physics-informed deep learning framework to address common limitations in Confocal Laser Scanning Microscopy (CLSM), such as diffraction limited resolution, noise, and undersampling due to low laser power conditions. The…

Materials Science · Physics 2025-01-27 Zaheer Ahmad , Junaid Shabeer , Usman Saleem , Tahir Qadeer , Abdul Sami , Zahira El Khalidi , Saad Mehmood

Reflectance Confocal Microscopy (RCM) is a non-invasive imaging technique used in biomedical research and clinical dermatology. It provides virtual high-resolution images of the skin and superficial tissues, reducing the need for physical…

Image and Video Processing · Electrical Eng. & Systems 2024-04-26 Hong-Jun Yoon , Chris Keum , Alexander Witkowski , Joanna Ludzik , Tracy Petrie , Heidi A. Hanson , Sancy A. Leachman

We report, for the first time, a multi-confocal Fluorescence Correlation Spectroscopy (mFCS) technique which allows parallel measurements at different locations, by combining a Spatial Light Modulator (SLM), with an Electron Multiplying-CCD…

Hyperspectral cameras face harsh trade-offs between spatial, spectral, and temporal resolution in inherently low-photon conditions. Computational imaging systems break through these trade-offs with compressive sensing, but have required…

Computer Vision and Pattern Recognition · Computer Science 2026-02-12 M. Kerem Aydin , Yi-Chun Hung , Jaclyn Pytlarz , Qi Guo , Emma Alexander

Fast, volumetric imaging over large scales has been a long-standing goal in biological microscopy. Scanning techniques such as fluorescence confocal microscopy can acquire 2D images at high resolution and high speed, but extending the…

Super-resolution optical microscopy is a rapidly evolving scientific field dedicated to imaging sub-wavelength sized objects, leaving its mark in multiple branches of biology and technology. While several super-resolution optical microscopy…

(Abridged) In the first part of this thesis, a general methodology for applying image deconvolution to wide-field CCD imagery. Results show that wavelet-based deconvolution can increase limiting magnitude up to 0.6 mag and improve limiting…

Astrophysics · Physics 2007-05-23 O. Fors

We introduce a novel laser-scanning optical microscopy technique that employs optical-frequency-comb (OFC) lasers. This method facilitates multimodal spectroscopic imaging by analyzing interferograms produced via a dual-comb spectroscopic…

We report on a program of spectroscopic observations of gravitationally-lensed QSOs with multiple images. We seek to establish whether microlensing is occurring in each QSO image using only single-epoch observations. We calculate flux…

Cosmology and Nongalactic Astrophysics · Physics 2015-06-05 V. Motta , E. Mediavilla , E. Falco , J. A. Munoz

A multispectral image camera captures image data within specific wavelength ranges in narrow wavelength bands across the electromagnetic spectrum. Images from a multispectral camera can extract additional information that the human eye or a…

Imaging specimens over large scales and with a sub-micron resolution is instrumental to biomedical research. Yet, the number of pixels to form such an image usually exceeds the number of pixels provided by conventional cameras. While most…

Instrumentation and Detectors · Physics 2019-12-03 Gaelle Recher , Pierre Nassoy , Amaury Badon

Twenty-one centimeter tomography is emerging as a powerful tool to explore the end of the cosmic dark ages and the reionization epoch, but it will only be as good as our ability to accurately model and remove astrophysical foreground…

Astrophysics · Physics 2008-11-26 Xiaomin Wang , Max Tegmark , Mario Santos , Lloyd Knox