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Roughly half of a cells proteins are located at or near the plasma membrane. In this restricted space the cell senses its environment, signals to its neighbors and ex-changes cargo through exo- and endocytotic mechanisms. Ligands bind to…
Luminescence imaging is invaluable for studying biological and material systems, particularly when advanced protocols that exploit temporal dynamics are employed. However, implementing such protocols often requires custom instrumentation,…
An integrated microscope that combines different optical techniques for simultaneous imaging is demonstrated. The microscope enables spectral-domain optical coherence microscopy based on optical backscatter, and multi-photon microscopy for…
Fluorescence microscopy is widely employed for the analysis of living biological samples; however, the utility of the resulting recordings is frequently constrained by noise, temporal variability, and inconsistent visualisation of signals…
Super-resolution imaging methods that combine interferometric (z) analysis with single-molecule localization microscopy (iSMLM) have achieved ultra-high 3D precision and contributed to the elucidation of important biological…
Interaction-free measurement (IFM) has been proposed as a means of high-resolution, low-damage imaging of radiation-sensitive samples, such as biomolecules and proteins. The basic setup for IFM is a Mach-Zehnder interferometer, and recent…
Measuring the three dimension nanoscale organization of protein or cellular structures is challenging, especially when the structure is dynamic. Owing to the informative total internal reflection fluorescence (TIRF) imaging under varied…
Label-free nonlinear optical microscopy has become a powerful tool for biomedical research. However, the possible photodamage risk hinder further clinical applications. To reduce these adverse effects, we constructed a new platform of…
Intraoperative optical imaging is essential for surgical precision and patient safety, but current systems present anatomical and fluorescence information separately, causing delays and increasing cognitive load. A unified system for…
Digital holographic microscopy is a thriving imaging modality that attracted considerable research interest in quantitative biological cell imaging due to its ability to not only create excellent label-free contrast, but also supply…
Amyloid proteins are associated with a broad spectrum of neurodegenerative diseases. However, it remains a grand challenge to extract molecular structure information from intracellular amyloid proteins in their native cellular environment.…
Infrared and visible light image fusion aims to combine the strengths of both modalities to generate images that are rich in information and fulfill visual or computational requirements. This paper proposes an image fusion method based on…
Lensless illumination single-pixel imaging with a multicore fiber (MCF) is a computational imaging technique that enables potential endoscopic observations of biological samples at cellular scale. In this work, we show that this technique…
Selective plane illumination microscopy (SPIM), also known as light sheet fluorescence microscopy, provides high specificity through fluorescence labeling. However, it lacks complementary structural information from the surrounding context,…
More than twenty years ago, scientists succeeded in pushing the limits of optical detection to single molecules using fluorescence. This breakthrough has revolutionized biophysical measurements, but restrictions in photophysics and labeling…
Mid-infrared photothermal (MIP) microscopy has been a promising label-free chemical imaging technique for functional characterization of specimens owing to its enhanced spatial resolution and high specificity. Recently developed wide-field…
Dynamic full-field optical coherence microscopy (d-FF-OCM) is a label-free imaging technique that captures intrinsic subcellular motions to generate functional contrast. This dynamic approach yields images with fluorescence-like contrast,…
Absolute quantity imaging of biomolecules on a single cell level is critical for measurement assurance in biosciences and bioindustries. While infrared (IR) transmission microscopy is a powerful label-free imaging modality capable of…
Imaging mass spectrometry (IMS) is a powerful tool for untargeted, highly multiplexed molecular mapping of tissue in biomedical research. IMS offers a means of mapping the spatial distributions of molecular species in biological tissue with…
Stimulated Raman scattering (SRS) microscopy offers great potential to surpass fluorescent-based approaches, owing to the sharp linewidth of Raman vibrations amenable to super-multiplex cell imaging, but currently lacks one crucial…