Related papers: Deconvolution in Fluorescence Lifetime imaging mic…
Fluorescence microscopy images usually show severe anisotropy in axial versus lateral resolution. This hampers downstream processing, i.e. the automatic extraction of quantitative biological data. While deconvolution methods and other…
This paper demonstrates a practical method that can correct spatial varying blur from a set of images of the same object. The algorithm jointly estimates the object and local point spread functions~(PSF). The method prioritizes sections…
The diffraction of light imposes a fundamental limit on the resolution of light microscopes. This limit can be circumvented by creating and exploiting independent behaviors of the sample at length scales below the diffraction limit. In…
Low-light optical imaging refers to the use of cameras to capture images with minimal photon flux. This area has broad application to diverse fields, including optical microscopy for biological studies. In such studies, it is important to…
Fluorescence microscopy (FM) imaging is a fundamental technique for observing live cell division, one of the most essential processes in the cycle of life and death. Observing 3D live cells requires scanning through the cell volume while…
Confocal laser scanning microscopy (CLSM) stands out as one of the most widely used microscopy techniques, thanks to its three-dimensional imaging capability and its sub-diffraction spatial resolution, achieved through the closure of a…
The quality of microscopy images often suffers from optical aberrations. These aberrations and their associated point spread functions have to be quantitatively estimated to restore aberrated images. The recent state-of-the-art method…
Autofluorescence lifetime images reveal unique characteristics of endogenous fluorescence in biological samples. Comprehensive understanding and clinical diagnosis rely on co-registration with the gold standard, histology images, which is…
We describe the effect of optical aberrations on fluorescence fluctuations microscopy (FFM), when focusing through a single living cell. FFM measurements are performed in an aqueous fluorescent solution and prove to be a highly sensitive…
Breaking the diffraction limit in microscopy by utilizing quantum properties of light has been the goal of intense research in the recent years. We propose a quantum superresolution technique based on non-classical emission statistics of…
Far-field optical microscopy using focused light is an important tool in a number of scientific disciplines including chemical, (bio)physical and biomedical research, particularly with respect to the study of living cells and organisms.…
Super-resolution microscopy is crucial for imaging sub-wavelength biological structures. However, most techniques rely on nonlinear saturation or stochastic switching of emitters, limiting imaging speed and increasing phototoxicity. Here,…
Single-pixel imaging has emerged as a key technique in fluorescence microscopy, where fast acquisition and reconstruction are crucial. In this context, images are reconstructed from linearly compressed measurements. In practice, total…
In this paper we consider a statistical estimation problem known as atomic deconvolution. Introduced in reliability, this model has a direct application when considering biological data produced by flow cytometers. In these experiments,…
Cryo-electron microscopy (EM) single particle reconstruction is an entirely general technique for 3D structure determination of macromolecular complexes. However, because the images are taken at low electron dose, it is extremely hard to…
The combination of different imaging modalities into single imaging platforms has a strong potential in biomedical sciences since it permits the analysis of complementary properties of the target sample. Here, we report on an extremely…
A new technique is presented for producing images from interferometric data. The method, ``smear fitting'', makes the constraints necessary for interferometric imaging double as a model, with uncertainties, of the sky brightness…
Lensless microscopy with coherent or partially coherent light sources is a well known imaging technique, commonly referred as digital in-line holographic microscopy. In the established methods, both the spatial and temporal coherence of…
Richardson-Lucy deconvolution is widely used to restore images from degradation caused by the broadening effects of a point spread function and corruption by photon shot noise, in order to recover an underlying object. In practice, this is…
We study the problem of deconvolution for light-sheet microscopy, where the data is corrupted by spatially varying blur and a combination of Poisson and Gaussian noise. The spatial variation of the point spread function (PSF) of a…