Related papers: Deconvolution in Fluorescence Lifetime imaging mic…
Super-resolution microscopy is rapidly gaining importance as an analytical tool in the life sciences. A compelling feature is the ability to label biological units of interest with fluorescent markers in living cells and to observe them…
Super-resolution microscopy has catalyzed valuable insights into the sub-cellular, mechanistic details of many different biological processes across a wide range of cell types. Fluorescence polarization spectroscopy tools have also enabled…
We present a method for beam deconvolution for cosmic microwave background (CMB) anisotropy measurements. The code takes as input the time-ordered data, along with the corresponding detector pointings and known beam shapes, and produces as…
Structured Illumination Microscopy (SIM) allows access to spatial information beyond the diffraction limit by folding high frequency components into the optical system's base-band. Using various algorithmic techniques, an image containing…
We present an imaging technique that allows the recovery of the transparency profile of wavelength-scale objects with deep subwavelength resolution based on far-field intensity measurements. The approach, interscale mixing microscopy (IMM),…
Fluorescence microscopy is rapidly turning into nanoscopy. Among the various nanoscopy methods, the STED/RESOLFT super-resolution family has recently been expanded to image even large fields of view within a few seconds. This advance relies…
We propose a chiral imaging modality based on optical chirality engineering, fluorescence-detected circular dichroism and structured illumination microscopy. In this method, the optical chirality of the illumination is structured and the…
In the imaging process of an astronomical telescope, the deconvolution of its beam or Point Spread Function (PSF) is a crucial task. However, deconvolution presents a classical and challenging inverse computation problem. In scenarios where…
Deconvolution algorithms have been used successfully for optimization/restoration/deblurring of astronomical images in a variety of wavelengths, especially in the optical band (e.g., for HST). We present here an iterative Richardson-Lucy…
In traditional optical imaging systems, the spatial resolution is limited by the physics of diffraction, which acts as a low-pass filter. The information on sub-wavelength features is carried by evanescent waves, never reaching the camera,…
In this paper, a compact and low-cost structured illumination microscope (SIM) based on a 2X2 fiber coupler is presented. Fringe illumination is achieved by placing two output fiber tips at a conjugate Fourier plane of the sample plane as…
We combine confocal imaging, microfluidics and image analysis to record 3D-images of cells in flow. This enables us to recover the full 3D representation of several hundred living cells per minute. Whereas 3D confocal imaging has thus far…
Fluorescence microscopy is widely used in biological imaging, however scattering from tissues strongly limits its applicability to a shallow depth. In this work we adapt a methodology inspired from stellar speckle interferometry, and…
Light-field microscopy represents a promising solution for microscopic volumetric imaging, thanks to its capability to encode information on multiple planes in a single acquisition. This is achieved through its peculiar simultaneous capture…
Structured illumination microscopy (SIM) is a pivotal technique for dynamic subcellular imaging in live cells. Conventional SIM reconstruction algorithms depend on accurately estimating the illumination pattern and can introduce artefacts…
This study introduces a novel data-centric approach to improve real-time surgical guidance using fiber-based fluorescence lifetime imaging (FLIm). A key aspect of the methodology is the accurate detection of the aiming beam, which is…
Fluorescence microscopy is of vital importance for understanding biological function. However most fluorescence experiments are only qualitative inasmuch as the absolute number of fluorescent particles can often not be determined.…
We leverage deep learning techniques to jointly denoise and super-resolve biomedical images acquired with fluorescence microscopy. We develop a deep learning algorithm based on the networks and method described in the recent W2S paper to…
Fluorescence molecular tomography (FMT) is an emerging powerful tool for biomedical research. There are two factors that influence FMT reconstruction most effectively. The first one is the regularization techniques. Traditional methods such…
Digital holographic microscopy (DHM) applied to quantitative phase imaging (QPI) has been successfully demonstrated as a powerful label-free method to analyse the optical properties of cells. Spatially multiplexed interferometric microscopy…