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Fluorescence lifetime imaging microscopy (FLIM) systems are limited by their slow processing speed, low signal-to-noise ratio (SNR), and expensive and challenging hardware setups. In this work, we demonstrate applying a denoising…

Image and Video Processing · Electrical Eng. & Systems 2021-06-09 Varun Mannam , Yide Zhang , Xiaotong Yuan , Takashi Hato , Pierre C. Dagher , Evan L. Nichols , Cody J. Smith , Kenneth W. Dunn , Scott Howard

AI-assisted nuclei segmentation in histopathological images is a crucial task in the diagnosis and treatment of cancer diseases. It decreases the time required to manually screen microscopic tissue images and can resolve the conflict…

Image and Video Processing · Electrical Eng. & Systems 2023-11-21 Hesham Ali , Idriss Tondji , Mennatullah Siam

Recently, a popular line of research in face recognition is adopting margins in the well-established softmax loss function to maximize class separability. In this paper, we first introduce an Additive Angular Margin Loss (ArcFace), which…

Computer Vision and Pattern Recognition · Computer Science 2022-09-07 Jiankang Deng , Jia Guo , Jing Yang , Niannan Xue , Irene Kotsia , Stefanos Zafeiriou

Despite being the main tool to visualize molecules at the atomic scale, AFM with CO-functionalized metal tips is unable to chemically identify the observed molecules. Here we present a strategy to address this challenging task using deep…

Materials Science · Physics 2025-09-03 Jaime Carracedo-Cosme , Carlos Romero-Muñiz , Pablo Pou , Rubén Pérez

Scanning electron microscopy (SEM) is indispensable in diverse applications ranging from microelectronics to food processing because it provides large depth-of-field images with a resolution beyond the optical diffraction limit. However,…

Flow-Imaging Microscopy (FIM) is commonly used in both academia and industry to characterize subvisible particles (those $\le 25 \mu m$ in size) in protein therapeutics. Pharmaceutical companies are required to record vast volumes of FIM…

Quantitative Methods · Quantitative Biology 2017-09-04 Christopher P. Calderon , Austin L. Daniels , Theodore W. Randolph

Computational imaging methods empower modern microscopy with the ability of producing high-resolution, large field-of-view, aberration-free images. One of the dominant computational label-free imaging methods, Fourier ptychographic…

Optics · Physics 2023-09-06 Ruizhi Cao , Cheng Shen , Changhuei Yang

Anterior segment optical coherence tomography (AS-OCT) is a non-invasive imaging technique that is highly valuable for ophthalmic diagnosis. However, speckles in AS-OCT images can often degrade the image quality and affect clinical…

Graphics · Computer Science 2023-07-03 Li Sanqian , Higashita Risa , Fu Huazhu , Li Heng , Niu Jingxuan , Liu Jiang

Medical image segmentation, or computing voxelwise semantic masks, is a fundamental yet challenging task to compute a voxel-level semantic mask. To increase the ability of encoder-decoder neural networks to perform this task across large…

Computer Vision and Pattern Recognition · Computer Science 2021-11-10 Ho Hin Lee , Yucheng Tang , Qi Yang , Xin Yu , Shunxing Bao , Leon Y. Cai , Lucas W. Remedios , Bennett A. Landman , Yuankai Huo

This study introduces a hybrid approach integrating advanced plasmonic nanomaterials and machine learning (ML) for high-precision biomolecule detection. We leverage aluminum concave nanocubes (AlCNCs) as an innovative plasmonic substrate to…

Biomolecules · Quantitative Biology 2025-07-11 Mohammad Mohammadi , Sima Najafzadehkhoei , George Vega Yon , Yunshan Wang

Semantic segmentation is a crucial task in medical imaging. Although supervised learning techniques have proven to be effective in performing this task, they heavily depend on large amounts of annotated training data. The recently…

Computer Vision and Pattern Recognition · Computer Science 2024-11-20 Ron Keuth , Lasse Hansen , Maren Balks , Ronja Jäger , Anne-Nele Schröder , Ludger Tüshaus , Mattias Heinrich

Dynamic full-field optical coherence microscopy (d-FF-OCM) is a label-free imaging technique that captures intrinsic subcellular motions to generate functional contrast. This dynamic approach yields images with fluorescence-like contrast,…

Optics · Physics 2025-08-06 Erikas Tarvydas , Austeja Treciokaite , Egidijus Auksorius

Real world images often have highly imbalanced content density. Some areas are very uniform, e.g., large patches of blue sky, while other areas are scattered with many small objects. Yet, the commonly used successive grid downsampling…

Computer Vision and Pattern Recognition · Computer Science 2023-10-27 Chen Ziwen , Kaushik Patnaik , Shuangfei Zhai , Alvin Wan , Zhile Ren , Alex Schwing , Alex Colburn , Li Fuxin

Autofocus (AF) methods are extensively used in biomicroscopy, for example to acquire timelapses, where the imaged objects tend to drift out of focus. AD algorithms determine an optimal distance by which to move the sample back into the…

Image and Video Processing · Electrical Eng. & Systems 2021-01-14 Adrian Shajkofci , Michael Liebling

Confocal laser-scanning microscopy (CLSM) is one of the most popular optical architectures for fluorescence imaging. In CLSM, a focused laser beam excites the fluorescence emission from a specific specimen position. Some actuators scan the…

Image and Video Processing · Electrical Eng. & Systems 2023-05-10 Alessandro Zunino , Marco Castello , Giuseppe Vicidomini

The Segment Anything Model (SAM) exhibits a capability to segment a wide array of objects in natural images, serving as a versatile perceptual tool for various downstream image segmentation tasks. In contrast, medical image segmentation…

Computer Vision and Pattern Recognition · Computer Science 2023-08-29 Yizhe Zhang , Tao Zhou , Shuo Wang , Ye Wu , Pengfei Gu , Danny Z. Chen

FRET-based approaches are a unique tool for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging and FLIM (Fluorescence Lifetime Imaging Microscopy) enable the visualization of the spatial distribution of…

Obtaining pixel-level annotations in the medical domain is both expensive and time-consuming, often requiring close collaboration between clinical experts and developers. Semi-supervised medical image segmentation aims to leverage limited…

Computer Vision and Pattern Recognition · Computer Science 2025-07-23 Lin Xi , Yingliang Ma , Cheng Wang , Sandra Howell , Aldo Rinaldi , Kawal S. Rhode

Label-free imaging of rapidly moving, sub-diffraction sized structures has important applications in both biology and material science, as it removes the limitations associated with fluorescence tagging. However, unlabeled nanoscale…

Reflectance confocal microscopy (RCM) is an effective, non-invasive pre-screening tool for skin cancer diagnosis, but it requires extensive training and experience to assess accurately. There are few quantitative tools available to…

Computer Vision and Pattern Recognition · Computer Science 2017-12-04 Alican Bozkurt , Kivanc Kose , Jaume Coll-Font , Christi Alessi-Fox , Dana H. Brooks , Jennifer G. Dy , Milind Rajadhyaksha