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Related papers: Artificial confocal microscopy for deep label-free…

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Confocal and multi-photon microscopy are widely used for in-vivo fluorescence imaging of biological tissues such as the brain, offering non-invasive access up to ~1 mm depth without major loss in performance. A recently-developed…

One primary technical challenge in photoacoustic microscopy (PAM) is the necessary compromise between spatial resolution and imaging speed. In this study, we propose a novel application of deep learning principles to reconstruct…

Image and Video Processing · Electrical Eng. & Systems 2020-06-02 Anthony DiSpirito , Daiwei Li , Tri Vu , Maomao Chen , Dong Zhang , Jianwen Luo , Roarke Horstmeyer , Junjie Yao

Medical image segmentation is a fundamental yet challenging task due to the arduous process of acquiring large volumes of high-quality labeled data from experts. Contrastive learning offers a promising but still problematic solution to this…

Computer Vision and Pattern Recognition · Computer Science 2025-02-14 Shuang Zeng , Lei Zhu , Xinliang Zhang , Micky C Nnamdi , Wenqi Shi , J Ben Tamo , Qian Chen , Hangzhou He , Lujia Jin , Zifeng Tian , Qiushi Ren , Zhaoheng Xie , Yanye Lu

Ultrasound vascular imaging is limited by acoustic diffraction, restricting visualization of microvessels essential for understanding organ function and disease. Label-free super-resolution methods exploiting endogenous red blood cells have…

Objective: Ultrahigh-resolution optical coherence microscopy (OCM) has recently demonstrated its potential for accurate diagnosis of human cervical diseases. One major challenge for clinical adoption, however, is the steep learning curve…

Computer Vision and Pattern Recognition · Computer Science 2020-04-28 Yutao Ma , Tao Xu , Xiaolei Huang , Xiaofang Wang , Canyu Li , Jason Jerwick , Yuan Ning , Xianxu Zeng , Baojin Wang , Yihong Wang , Zhan Zhang , Xiaoan Zhang , Chao Zhou

Deep networks achieve excellent results on large-scale clean data but degrade significantly when learning from noisy labels. To suppressing the impact of mislabeled data, this paper proposes a conceptually simple yet efficient training…

Computer Vision and Pattern Recognition · Computer Science 2020-10-30 Xiaojiang Peng , Kai Wang , Zhaoyang Zeng , Qing Li , Jianfei Yang , Yu Qiao

Optical nanoscopy is crucial in life and materials sciences, revealing subtle cellular processes and nanomaterial properties. Scattering-type Scanning Near-field Optical Microscopy (s-SNOM) provides nanoscale resolution, relying on the…

Label-free imaging has gained broad interest because of its potential to omit elaborate staining procedures which is especially relevant for in vivo use. Label-free multiphoton microscopy (MPM), for instance, exploits two-photon excitation…

Confocal microscopy, a critical advancement in optical imaging, is widely applied because of its excellent anti-noise ability. However, it has low imaging efficiency and can cause phototoxicity. Optical-sectioning structured illumination…

Optics · Physics 2024-05-27 Weishuai Zhou , Manhong Yao , Xi Lin , Quan Yu , Junzheng Peng , Jingang Zhong

Automatic prediction of fluorescently labeled organelles from label-free transmitted light input images is an important, yet difficult task. The traditional way to obtain fluorescence images is related to performing biochemical labeling…

Image and Video Processing · Electrical Eng. & Systems 2024-06-04 Marek Wodzinski , Henning Müller

In this work we address the task of semantic image segmentation with Deep Learning and make three main contributions that are experimentally shown to have substantial practical merit. First, we highlight convolution with upsampled filters,…

Computer Vision and Pattern Recognition · Computer Science 2017-05-15 Liang-Chieh Chen , George Papandreou , Iasonas Kokkinos , Kevin Murphy , Alan L. Yuille

We demonstrate sub-wavelength sectioning on biological samples with a conventional confocal microscope. This optical sectioning is achieved by the phenomenon of supercritical angle fuorescence, wherein only a fluorophore next to the…

Automated and semi-automated techniques in biomedical electron microscopy (EM) enable the acquisition of large datasets at a high rate. Segmentation methods are therefore essential to analyze and interpret these large volumes of data, which…

Computer Vision and Pattern Recognition · Computer Science 2023-08-08 Anusha Aswath , Ahmad Alsahaf , Ben N. G. Giepmans , George Azzopardi

Weakly-supervised diffusion models (DMs) in anomaly segmentation, leveraging image-level labels, have attracted significant attention for their superior performance compared to unsupervised methods. It eliminates the need for pixel-level…

Computer Vision and Pattern Recognition · Computer Science 2025-01-09 Yiming Che , Fazle Rafsani , Jay Shah , Md Mahfuzur Rahman Siddiquee , Teresa Wu

Multifocus microscopy enables recording of entire volumes in a single camera exposure. In dense samples, multifocus microscopy is severely hampered by background haze. Here, we introduce a scalable multifocus method that incorporates…

Semi-supervised learning has become increasingly popular in medical image segmentation due to its ability to leverage large amounts of unlabeled data to extract additional information. However, most existing semi-supervised segmentation…

Computer Vision and Pattern Recognition · Computer Science 2023-07-13 Shengbo Gao , Ziji Zhang , Jiechao Ma , Zihao Li , Shu Zhang

We introduce HAMscope, a compact, snapshot hyperspectral autofluorescence miniscope that enables real-time, label-free molecular imaging in a wide range of biological systems. By integrating a thin polymer diffuser into a widefield…

High-quality pixel-level annotations of medical images are essential for supervised segmentation tasks, but obtaining such annotations is costly and requires medical expertise. To address this challenge, we propose a novel coarse-to-fine…

Computer Vision and Pattern Recognition · Computer Science 2025-08-06 Anghong Du , Nay Aung , Theodoros N. Arvanitis , Stefan K. Piechnik , Joao A C Lima , Steffen E. Petersen , Le Zhang

The segmentation of endoscopic images plays a vital role in computer-aided diagnosis and treatment. The advancements in deep learning have led to the employment of numerous models for endoscopic tumor segmentation, achieving promising…

Computer Vision and Pattern Recognition · Computer Science 2025-02-03 Lingcong Cai , Yun Li , Xiaomao Fan , Kaixuan Song , Ruxin Wang , Wenbin Lei

Atomic Force Microscopy (AFM) operating in the frequency modulation mode with a metal tip functionalized with a CO molecule images the internal structure of molecules with an unprecedented resolution. The interpretation of these images is…

Materials Science · Physics 2022-12-29 Jaime Carracedo-Cosme , Rubén Pérez