Related papers: Combining fluorescence fluctuations and photobleac…
FRET measurements can provide dynamic spatial information on length scales smaller than the diffraction limit of light. Several methods exist to measure FRET between fluorophores, including Fluorescence Lifetime Imaging Microscopy (FLIM),…
Label-free detecting multiple analytes in a high-throughput fashion has been one of the long-sought goals in biosensing applications. Yet, for all-optical approaches, interfacing state-of-the-art label-free techniques with microfluidics…
(Abridged) We describe a new method to extract spectra of stars from observations of crowded stellar fields with integral field spectroscopy (IFS). Our approach extends the well-established concept of crowded field photometry in images into…
Most scenes are illuminated by several light sources, where the traditional assumption of uniform illumination is invalid. This issue is ignored in most color constancy methods, primarily due to the complex spatial impact of multiple light…
Fluorescence microscopy allows for a detailed inspection of cells, cellular networks, and anatomical landmarks by staining with a variety of carefully-selected markers visualized as color channels. Quantitative characterization of…
There is currently a renewed interest in the development of experimental methods to achieve the elastic characterization of thin films, multilayers and acoustic resonators operating in the GHz range of frequencies. The potentialities of…
The per- and polyfluoroalkyl substances (PFAS) constitute a group of organofluorine chemicals treated as the emerging pollutants and currently are of particularly acute concern. These compounds have been employed intensively as surfactants…
We propose the DPSM method, a density-based node clustering approach that automatically determines the number of clusters and can be applied in both data space and graph space. Unlike traditional density-based clustering methods, which…
Far-field super-resolution fluorescence microscopy has been rapidly developed for applications ranging from cell biology to nanomaterials. However, it remains a significant challenge to achieve super-resolution imaging at depth in opaque…
The photon density operator function is used to calculate light beam propagation through turbulent atmosphere. A kinetic equation for the photon distribution function is derived and solved using the method of characteristics. Optical wave…
Fermi surfaces, three-dimensional (3D) abstract interfaces that define the occupied energies of electrons in a solid, are important for characterizing and predicting the thermal, electrical, magnetic, and optical properties of crystalline…
The Coarse-to-Fine Few-Shot (C2FS) task is designed to train models using only coarse labels, then leverages a limited number of subclass samples to achieve fine-grained recognition capabilities. This task presents two main challenges:…
The accuracy in the photometry of a point source depends on the point-spread function (PSF), detector pixelization, and observing strategy. The PSF and pixel response describe the spatial blurring of the source, the pixel scale describes…
The determination of minority-carrier lifetimes and surface recombination velocities is essential for the development of semiconductor technologies such as solar cells. The recent development of two-photon time-resolved microscopy allows…
Confocal microscopy of fluorescent labeled particles has been used to study the dynamical and structural properties of colloidal and granular matter in real space. Localization algorithms allow for a fully automatized determination of the…
We experimentally study spatial fluctuations of the local density of states (LDOS) inside three-dimensional random photonic media. The LDOS is probed at many positions inside the random photonic media by measuring emission rates of a large…
We report on a recently developed laser-probing diagnostic which allows direct measurements of ray-deflection angles in one axis, whilst retaining imaging capabilities in the other axis. This allows us to measure the spectrum of angular…
Point-wise localization of individual fluorophores is a critical step in super-resolution microscopy and single particle tracking. Although the methods are limited by the accuracy in localizing individual flourophores, this point-wise…
We propose the use of a frequency-dependent photothermal measurement as a complement to light-flash, i.e. time-dependent, measurements to determine the through-plane thermal diffusivity of small, thin samples, e.g. semiconducting polymers…
In this two-part paper, we present a novel framework and methodology to analyze data from certain image-based biochemical assays, e.g., ELISPOT and Fluorospot assays. In this first part, we start by presenting a physical partial…