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The full understanding of cellular functions requires information about protein numbers for various biomolecular assemblies and their dynamics, which can be partly accessed by super-resolution fluorescence microscopy. Yet, many protein…

Biological Physics · Physics 2020-06-17 Kristin S. Grußmayer , Klaus Yserentant , Dirk-Peter Herten

Although several optical techniques have been recently developed in order to overcome the resolution limit in microscopy, the imaging of sub-wavelength features is still a real challenge. In practise, super-resolution techniques remain…

Physics Education · Physics 2019-07-24 Stephane Perrin , Keshia Badu , Paul Montgomery , Sylvain Lecler

Optical cavities are of central importance in numerous areas of physics, including precision measurement, cavity optomechanics and cavity quantum electrodynamics. The miniaturisation and scaling to large numbers of sites is of interest for…

Quantum Physics · Physics 2014-10-23 C. Derntl , M. Schneider , J. Schalko , A. Bittner , J. Schmiedmayer , U. Schmid , M. Trupke

Hyperspectral microscopy is an imaging technique that provides spectroscopic information with high spatial resolution. When applied in the relevant wavelength region, such as in the infrared (IR), it can reveal a rich spectral fingerprint…

In today's world of big data, computational analysis has become a key driver of biomedical research. Recent exponential growth in the volume of available omics data has reshaped the landscape of contemporary biology, creating demand for a…

Distributed, Parallel, and Cluster Computing · Computer Science 2019-12-09 Jaqueline J. Brito , Thiago Mosqueiro , Jeremy Rotman , Victor Xue , Douglas J. Chapski , Juan De la Hoz , Paulo Matias , Lana Martin , Alex Zelikovsky , Matteo Pellegrinni , Serghei Mangul

Electrochemistry exploits local current heterogeneities at various scales ranging from the micrometer to the nanometer. The last decade has witnessed unprecedented progress in the development of a wide range of electroanalytical techniques…

Optics · Physics 2022-11-16 Frederic Kanoufi

Biology is data-rich, and it is equally rich in concepts and hypotheses. Part of trying to understand biological processes and systems is therefore to confront our ideas and hypotheses with data using statistical methods to determine the…

Quantitative Methods · Quantitative Biology 2022-06-22 Sean T. Vittadello , Michael P. H. Stumpf

Video microscopy has a long history of providing insights and breakthroughs for a broad range of disciplines, from physics to biology. Image analysis to extract quantitative information from video microscopy data has traditionally relied on…

Image and Video Processing · Electrical Eng. & Systems 2024-06-12 Benjamin Midtvedt , Saga Helgadottir , Aykut Argun , Jesús Pineda , Daniel Midtvedt , Giovanni Volpe

Current in vivo microscopy allows us detailed spatiotemporal imaging (3D+t) of complete organisms and offers insights into their development on the cellular level. Even though the imaging speed and quality is steadily improving,…

Image and Video Processing · Electrical Eng. & Systems 2020-01-31 Sourabh Bhide , Ralf Mikut , Maria Leptin , Johannes Stegmaier

Large collections of high-dimensional data have become nearly ubiquitous across many academic fields and application domains, ranging from biology to the humanities. Since working directly with high-dimensional data poses challenges, the…

Microorganisms play a vital role in human life. Therefore, microorganism detection is of great significance to human beings. However, the traditional manual microscopic detection methods have the disadvantages of long detection cycle, low…

Computer Vision and Pattern Recognition · Computer Science 2022-04-12 Pingli Ma , Chen Li , Md Mamunur Rahaman , Yudong Yao , Jiawei Zhang , Shuojia Zou , Xin Zhao , Marcin Grzegorzek

For the past few decades, structured light has been gaining popularity across various research fields. Its fascinating properties have been exploited for both previously unforeseen and established applications from new perspectives. Crucial…

Optics · Physics 2024-10-23 Carmelo Rosales-Guzmán , Valeria Rodriguez-Fajardo

The ability to represent scientific data and concepts visually is becoming increasingly important due to the unprecedented exponential growth of computational power during the present digital age. The data sets and simulations scientists in…

Instrumentation and Methods for Astrophysics · Physics 2009-11-18 Alyssa Goodman

The study of microorganisms, or microbiology, has demonstrated significant development since its inception and is currently a key field of biological sciences that has a huge impact on modern society and scientific research. Over the…

Biological Physics · Physics 2025-05-16 Daniil S. Gerassimov

Expansion Microscopy is a super-resolution technique in which physically enlarging samples in an isotropic manner increases inter-molecular distances such that nano-scale structures can be resolved using light microscopy. This is…

Neurons and Cognition · Quantitative Biology 2025-01-22 Shakila Behzadi , Jacquelin Ho , Zainab Tanvir , Gal Haspel , Limor Freifeld , Kristen E. Severi

The living body is composed of innumerable fine and complex structures and although these structures have been studied in the past, a vast amount of information pertaining to them still remains unknown. When attempting to observe these…

Medical Physics · Physics 2021-11-30 Raku Son , Kenji Yamazawa , Akiko Oguchi , Mitsuo Suga , Masaru Tamura , Yasuhiro Murakawa , Satoshi Kume

The physiological tradition of biological research analyzes biological systems using reduced descriptions much as an engineer uses a 'black box' description of an amplifier. Simple models have been used by physiologists for a very long…

Other Quantitative Biology · Quantitative Biology 2012-07-06 Bob Eisenberg

Modern imaging technologies are widely based on classical principles of light or electromagnetic wave propagation. They can be remarkably sophisticated, with recent successes ranging from single molecule microscopy to imaging far-distant…

Mass spectrometry is a widely used method to study molecules and processes in medicine, life sciences, chemistry, catalysis, and industrial product quality control, among many other applications. One of the main features of some mass…

Chemical Physics · Physics 2024-07-02 Daniil A. Boiko , Valentine P. Ananikov

Optical microscopy offers a unique insight of biological structures with a sub-micrometer resolution and a minimum invasiveness. However, the inhomogeneities of the specimen itself can induce multiple scattering of light and optical…