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Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization…

Fluorescence fluctuations-based super-resolution microscopy (FF-SRM) is an emerging field promising low-cost and live-cell compatible imaging beyond the resolution of conventional optical microscopy. A comprehensive overview on how the…

To overcome the physical barriers caused by light diffraction, super-resolution techniques are often applied in fluorescence microscopy. State-of-the-art approaches require specific and often demanding acquisition conditions to achieve…

Super-resolution light microscopy overcomes the physical barriers due to light diffraction, allowing for the observation of otherwise indistinguishable subcellular entities. However, the specific acquisition conditions required by…

Ultrasound images formed by delay-and-sum beamforming are plagued by artifacts that only clear up after compounding many transmissions. Some prior works pose imaging as an inverse problem. This approach can yield high image quality with few…

Signal Processing · Electrical Eng. & Systems 2024-07-03 Vincent van de Schaft , Oisín Nolan , Ruud J. G. van Sloun

Far-field super-resolution fluorescence microscopy has been rapidly developed for applications ranging from cell biology to nanomaterials. However, it remains a significant challenge to achieve super-resolution imaging at depth in opaque…

Optics · Physics 2024-05-01 Tengfei Wu , YoonSeok Baek , Fei Xia , Sylvain Gigan , Hilton B. de Aguiar

Improving the resolution of fluorescence microscopy beyond the diffraction limit can be achievedby acquiring and processing multiple images of the sample under different illumination conditions.One of the simplest techniques, Random…

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going "beyond the diffraction barrier" comes at a price since most far-field super-resolution imaging techniques…

Fluorescence microscopy has enabled a dramatic development in modern biology by visualizing biological organisms with micrometer scale resolution. However, due to the diffraction limit, sub-micron/nanometer features are difficult to…

Image and Video Processing · Electrical Eng. & Systems 2021-03-10 Varun Mannam , Yide Zhang , Xiaotong Yuan , Scott Howard

The recovery of Dirac impulses, or spikes, from filtered measurements is a classical problem in signal processing. As the spikes lie in the continuous domain while measurements are discrete, this task is known as super-resolution or…

Information Theory · Computer Science 2025-10-21 Ruiming Guo , Ayush Bhandari

Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution…

Biological Physics · Physics 2007-08-27 James H. Rice

We numerically investigate the role of quantum fluctuations in superresolution of optical objects. First, we confirm that when quantum fluctuations are not taken into account, one can easily improve the resolution by one order of magnitude…

Quantum Physics · Physics 2007-05-23 Vladislav N. Beskrovnyy , Mikhail I. Kolobov

We introduce a superresolution technique that combines spatial mode demultiplexing (SPADE) with emitter blinking. We show that temporal fluctuations not only enhance the precision of SPADE imaging, but also drastically simplify the…

Optics · Physics 2025-11-27 Stanislaw Kurdzialek

We propose a novel learning based algorithm to generate efficient and physically plausible sampling patterns in MRI. This method has a few advantages compared to recent learning based approaches: i) it works off-the-grid and ii) allows to…

Machine Learning · Computer Science 2020-10-06 Alban Gossard , Frédéric de Gournay , Pierre Weiss

The spatial resolution of images of living samples obtained by fluorescence microscopes is physically limited due to the diffraction of visible light, which makes the study of entities of size less than the diffraction barrier (around 200…

Image and Video Processing · Electrical Eng. & Systems 2023-03-21 Vasiliki Stergiopoulou , Subhadip Mukherjee , Luca Calatroni , Laure Blanc-Féraud

Super-resolution is the problem of recovering a superposition of point sources using bandlimited measurements, which may be corrupted with noise. This signal processing problem arises in numerous imaging problems, ranging from astronomy to…

Machine Learning · Computer Science 2015-09-29 Qingqing Huang , Sham M. Kakade

In some super-resolution techniques, adjacent points are illuminated at different times. Thereby, their locations and light intensities can be detected even if the images are very blurred due to diffraction. According to conventional…

Image and Video Processing · Electrical Eng. & Systems 2019-12-10 Edward Y. Sheffield

Second harmonic generation microscopy (SHG) is a powerful imaging modality which has found applications in investigating both biological and synthetic nanostructures. Like all optical microscopy techniques, the resolution of SHG is limited…

Fluorescence microscopy is rapidly turning into nanoscopy. Among the various nanoscopy methods, the STED/RESOLFT super-resolution family has recently been expanded to image even large fields of view within a few seconds. This advance relies…

We report an experimental demonstration of a nonclassical imaging mechanism with super-resolving power beyond the Rayleigh limit. When the classical image is completely blurred out due to the use of a small imaging lens, by taking advantage…

Optics · Physics 2016-11-03 Jane Sprigg , Tao Peng , Yanhua Shih
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