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Bacterial heterogeneity is pivotal for adaptation to diverse environments, posing significant challenges in microbial diagnostics and therapeutic interventions. Recent advancements in high-resolution optical microscopy have revolutionized…

Biological Physics · Physics 2025-01-06 Dohyeon Lee , Hyun-Seung Lee , Moosung Lee , Minhee Kang , Geon Kim , Tae Yeul Kim , Nam Yong Lee , YongKeun Park

Miniature fluorescence microscopes are a standard tool in systems biology. However, widefield miniature microscopes capture only 2D information, and modifications that enable 3D capabilities increase the size and weight and have poor…

Image and Video Processing · Electrical Eng. & Systems 2020-10-13 Kyrollos Yanny , Nick Antipa , William Liberti , Sam Dehaeck , Kristina Monakhova , Fanglin Linda Liu , Konlin Shen , Ren Ng , Laura Waller

Malaria, a mosquito-borne disease caused by a parasite, kills over 1 million people globally each year. People, if left untreated, may develop severe complications, leading to death. Effective and accurate diagnosis is important for the…

Image and Video Processing · Electrical Eng. & Systems 2020-09-02 Alexander Tao , Boran Han

In this article, we report an imaging method, termed Fourier ptychographic microscopy (FPM), which iteratively stitches together a number of variably illuminated, low-resolution intensity images in Fourier space to produce a wide-field,…

Optics · Physics 2014-05-02 Guoan Zheng , Roarke Horstmeyer , Changhuei Yang

Malaria parasites pose a significant global health burden, causing widespread suffering and mortality. Detecting malaria infection accurately is crucial for effective treatment and control. However, existing automated detection techniques…

Computer Vision and Pattern Recognition · Computer Science 2024-12-18 Abraham G Taye , Sador Yemane , Eshetu Negash , Yared Minwuyelet , Moges Abebe , Melkamu Hunegnaw Asmare

Malaria is a disease of global concern according to the World Health Organization. Billions of people in the world are at risk of Malaria today. Microscopy is considered the gold standard for Malaria diagnosis. Microscopic assessment of…

Image and Video Processing · Electrical Eng. & Systems 2020-12-01 Ruskin Raj Manku , Ayush Sharma , Anand Panchbhai

Malaria is a major health issue worldwide, and its diagnosis requires scalable solutions that can work effectively with low-cost microscopes (LCM). Deep learning-based methods have shown success in computer-aided diagnosis from microscopic…

Computer Vision and Pattern Recognition · Computer Science 2024-10-15 Ishan Rajendrakumar Dave , Tristan de Blegiers , Chen Chen , Mubarak Shah

Imaging of biological cells and tissues often relies on fluorescent labels, which offer high contrast with molecular specificity. The use of exogenous labeling agents, however, may alter the normal physiology of the bio-specimens.…

Instrumentation and Detectors · Physics 2019-10-01 Zichao Bian , Shaowei Jiang , Pengming Song , He Zhang , Pouria Hoveida , Kazunori Hoshino , Guoan Zheng

Fluorescence microscopy allows for a detailed inspection of cells, cellular networks, and anatomical landmarks by staining with a variety of carefully-selected markers visualized as color channels. Quantitative characterization of…

Computer Vision and Pattern Recognition · Computer Science 2021-08-26 Alvaro Gomariz , Tiziano Portenier , Patrick M. Helbling , Stephan Isringhausen , Ute Suessbier , César Nombela-Arrieta , Orcun Goksel

Autonomous migration is essential for the function of immune cells such as neutrophils and plays a pivotal role in diverse diseases. Recently, we introduced ComplexEye, a multi-lens array microscope comprising 16 independent…

Machine Learning · Computer Science 2025-11-19 Xiaowei Xu , Justin Sonneck , Hongxiao Wang , Roman Burkard , Hendrik Wohrle , Anton Grabmasier , Matthias Gunzer , Jianxu Chen

Magnetic Resonance Imaging (MRI), including diffusion MRI (dMRI), serves as a ``microscope'' for anatomical structures and routinely mitigates the influence of low signal-to-noise ratio scans by compromising temporal or spatial resolution.…

Image and Video Processing · Electrical Eng. & Systems 2025-03-11 Chenxu Wu , Qingpeng Kong , Zihang Jiang , S. Kevin Zhou

Open source hardware has the potential to revolutionise the way we build scientific instruments; with the advent of readily-available 3D printers, mechanical designs can now be shared, improved and replicated faster and more easily than…

Instrumentation and Detectors · Physics 2016-07-25 James P. Sharkey , Darryl C. W. Foo , Alexandre Kabla , Jeremy J. Baumberg , Richard W. Bowman

Recent advancements in whole slide imaging (WSI) have moved pathology closer to digital practice. Existing systems require precise mechanical control and the cost is prohibitive for most individual pathologists. Here we report a low-cost…

Image and Video Processing · Electrical Eng. & Systems 2019-12-30 Chengfei Guo , Zichao Bian , Shaowei Jiang , Michael Murphy , Jiakai Zhu , Ruihai Wang , Pengming Song , Xiaopeng Shao , Yongbing Zhang , Guoan Zheng

The capability of focus control has been central to optical technologies that require both high temporal and spatial resolutions. However, existing varifocal lens schemes are commonly limited to the response time on the microsecond…

Positioning patients for scanning and interventional procedures is a critical task that requires high precision and accuracy. The conventional workflow involves manually adjusting the patient support to align the center of the target body…

Computer Vision and Pattern Recognition · Computer Science 2024-12-10 Zhongpai Gao , Abhishek Sharma , Meng Zheng , Benjamin Planche , Terrence Chen , Ziyan Wu

Aggregation-based assays, using micro- and nano-particles have been widely accepted as an efficient and cost-effective bio-sensing tool, particularly in microbiology, where particle clustering events are used as a metric to infer the…

Instrumentation and Detectors · Physics 2018-12-31 Yichen Wu , Aniruddha Ray , Qingshan Wei , Alborz Feizi , Xin Tong , Eva Chen , Yi Luo , Aydogan Ozcan

Malaria, which primarily spreads with the bite of female anopheles mosquitos, often leads to death of people - specifically children in the age-group of 0-5 years. Clinical experts identify malaria by observing RBCs in blood smeared images…

Computer Vision and Pattern Recognition · Computer Science 2025-10-23 Warisa Nusrat , Mostafijur Rahman , Ayatullah Faruk Mollah

Automation in optical microscopy is critical for enabling high-throughput imaging across a wide range of biomedical applications. Among the essential components of automated systems, robust autofocusing plays a pivotal role in maintaining…

Label-free nonlinear optical microscopy has become a powerful tool for biomedical research. However, the possible photodamage risk hinder further clinical applications. To reduce these adverse effects, we constructed a new platform of…

Optics · Physics 2022-10-26 Geng Wang , Stephen A. Boppart , Haohua Tu

On-chip optical architectures that enable angle-resolved spectroscopy are essential for advancing photonic platforms towards low-volume, scalable, and cryo-compatible devices. Here, we introduce spatially resolved momentum-space imaging…