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Malaria is a life-threatening infectious disease caused by Plasmodium parasites, which poses a significant public health challenge worldwide, particularly in tropical and subtropical regions. Timely and accurate detection of malaria…

Image and Video Processing · Electrical Eng. & Systems 2024-05-24 Salam Ahmed Ali , Peshraw Salam Abdulqadir , Shan Ali Abdullah , Haruna Yunusa

Non-invasive morphological assessment is the cornerstone of oocyte and embryo selection in assisted reproductive technology, yet clinical practice remains limited by two-dimensional, qualitative microscopy. While three-dimensional (3D)…

Fluorescence microscopy is a widely used method among cell biologists for studying the localization and co-localization of fluorescent protein. For microbial cell biologists, these studies often include tedious and time-consuming manual…

Computer Vision and Pattern Recognition · Computer Science 2020-09-29 Jonathan Reiner , Guy Azran , Gal Hyams

Malaria remains one of the most pressing public health concerns globally, causing significant morbidity and mortality, especially in sub-Saharan Africa. Rapid and accurate diagnosis is crucial for effective treatment and disease management.…

Image and Video Processing · Electrical Eng. & Systems 2024-07-01 Saurabh Sawant , Anurag Singh

Standard microscopes offer a variety of settings to help improve the visibility of different specimens to the end microscope user. Increasingly, however, digital microscopes are used to capture images for automated interpretation by…

Image and Video Processing · Electrical Eng. & Systems 2020-10-14 Kanghyun Kim , Pavan Chandra Konda , Colin L. Cooke , Ron Appel , Roarke Horstmeyer

Oblique plane microscopy is a method enabling light-sheet fluorescence imaging through a single microscope objective lens by focusing on a tilted plane within the sample. To focus the fluorescence emitted by the oblique plane on a camera,…

Sparse-view reconstruction models typically require precise camera poses, yet obtaining these parameters from sparse-view images remains challenging. We introduce FreeSplatter, a scalable feed-forward framework that generates high-quality…

Computer Vision and Pattern Recognition · Computer Science 2025-09-03 Jiale Xu , Shenghua Gao , Ying Shan

Fourier ptychographic (FP) microscope is a coherent imaging method that can synthesize an image with a higher bandwidth using multiple low-bandwidth images captured at different spatial frequency regions. The method's demand for multiple…

Optics · Physics 2016-03-25 Jaebum Chung , Hangwen Lu , Xiaoze Ou , Haojiang Zhou , Changhuei Yang

We have developed a modular system for high-resolution microscopy at high hydrostatic pressure. The system consists of a pressurised cell of volume ~100 microlitres, a temperature controlled holder, a ram and a piston. We have made each of…

Optical microscopy is an indispensable tool in life sciences research, but conventional techniques require compromises between imaging parameters like speed, resolution, field-of-view, and phototoxicity. To overcome these limitations,…

Quantitative Methods · Quantitative Biology 2024-04-01 Leonor Morgado , Estibaliz Gómez-de-Mariscal , Hannah S. Heil , Ricardo Henriques

Light field microscopy (LFM) uses a microlens array (MLA) near the sensor plane of a microscope to achieve single-shot 3D imaging of a sample without any moving parts. Unfortunately, the 3D capability of LFM comes with a significant loss of…

Image and Video Processing · Electrical Eng. & Systems 2020-09-17 Fanglin Linda Liu , Grace Kuo , Nick Antipa , Kyrollos Yanny , Laura Waller

We propose a speed-up method for the in-focus plane detection in digital holographic microscopy that can be applied to a broad class of autofocusing algorithms that involve repetitive propagation of an object wave to various axial locations…

Quantitative Methods · Quantitative Biology 2023-05-19 Julianna Winnik , Damian Suski , Piotr Zdańkowski , Luiza Stanaszek , Vicente Micó , Maciej Trusiak

Multichannel, infinite-conjugate optical systems easily allow implementation of multiple image paths and imaging modes into a single microscope. Traditional optical alignment methods which rely on additional hardware are not always simple…

Optics · Physics 2024-07-16 Gemma S. Cairns , Brian R. Patton

Microscopy plays a crucial role in hematology and diagnosis of infectious diseases worldwide. For malaria alone, more than 200 million slides are read by manual microscopists every year. High quality thin blood smears are essential for…

Medical Physics · Physics 2023-04-21 Jerome Nowak , Anesta Kothari , Hongquan Li , Jaspreet Pannu , Dani Algazi , Manu Prakash

Monitoring plant health increasingly relies on leaf-mounted sensors that provide real-time physiological data, yet efficiently locating and sampling these sensors in complex agricultural environments remains a major challenge. We present an…

Image and Video Processing · Electrical Eng. & Systems 2025-12-12 Dexter Burns , Sanjeev Koppal

A targeted imaging system has been developed for applications requiring recording from stationary samples at high spatiotemporal resolutions. It works by illuminating regions of interest in rapid sequence, and recording the signal from the…

Fast 3D super-resolution imaging is essential for decoding rapidly occurring biological processes. Encoding single molecules to their respective planes enable simultaneous multi-plane super-resolution volume imaging. This saves the…

Biological Physics · Physics 2016-08-04 Partha Pratim Mondal

Three-dimensional (3D) fluorescence microscopy in general requires axial scanning to capture images of a sample at different planes. Here we demonstrate that a deep convolutional neural network can be trained to virtually refocus a 2D…

Computer Vision and Pattern Recognition · Computer Science 2019-11-05 Yichen Wu , Yair Rivenson , Hongda Wang , Yilin Luo , Eyal Ben-David , Laurent A. Bentolila , Christian Pritz , Aydogan Ozcan

Fast, volumetric imaging over large scales has been a long-standing goal in biological microscopy. Scanning techniques such as fluorescence confocal microscopy can acquire 2D images at high resolution and high speed, but extending the…

Accurate detection of Plasmodium falciparum in Giemsa-stained blood smears is an essential component of reliable malaria diagnosis, especially in developing countries. Deep learning-based object detection methods have demonstrated strong…

Computer Vision and Pattern Recognition · Computer Science 2025-11-17 Frauke Wilm , Luis Carlos Rivera Monroy , Mathias Öttl , Lukas Mürdter , Leonid Mill , Andreas Maier