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We introduce a new rotationally invariant viewing angle classification method for identifying, among a large number of Cryo-EM projection images, similar views without prior knowledge of the molecule. Our rotationally invariant features are…

Biomolecules · Quantitative Biology 2014-03-19 Zhizhen Zhao , Amit Singer

Cryo-electron microscopy (cryo-EM) is a powerful technique for determining the structure of proteins and other macromolecular complexes at near-atomic resolution. In single particle cryo-EM, the central problem is to reconstruct the…

Quantitative Methods · Quantitative Biology 2020-02-18 Ellen D. Zhong , Tristan Bepler , Joseph H. Davis , Bonnie Berger

We present a deep learning, computer vision algorithm constructed for the purposes of identifying and classifying charged particles in camera image sensors. We apply our algorithm to data collected by the Distributed Electronic Cosmic-ray…

X-ray single particle imaging involves the measurement of a large number of noisy diffraction patterns of isolated objects in random orientations. The missing information about these patterns is then computationally recovered in order to…

Image and Video Processing · Electrical Eng. & Systems 2020-05-25 Kartik Ayyer

We present a computational imaging mode for large scale electron microscopy data, which retrieves a complex wave from noisy/sparse intensity recordings using a deep learning approach and subsequently reconstructs an image of the specimen…

Materials Science · Physics 2022-02-28 Thomas Friedrich , Chu-Ping Yu , Johan Verbeek , Timothy Pennycook , Sandra Van Aert

Single particle imaging (SPI) at X-ray free electron lasers (XFELs) is a technique to determine the 3D structure of nanoscale objects like biomolecules from a large number of diffraction patterns of copies of these objects in random…

Image and Video Processing · Electrical Eng. & Systems 2022-10-20 Abhishek Mall , Kartik Ayyer

Confocal laser-scanning microscopy (CLSM) is one of the most popular optical architectures for fluorescence imaging. In CLSM, a focused laser beam excites the fluorescence emission from a specific specimen position. Some actuators scan the…

Image and Video Processing · Electrical Eng. & Systems 2023-05-10 Alessandro Zunino , Marco Castello , Giuseppe Vicidomini

Holography is a cornerstone characterisation and imaging technique that can be applied to the full electromagnetic spectrum, from X-rays to radio waves or even particles such as neutrons. The key property in all these holographic approaches…

Quantum Physics · Physics 2021-01-12 Hugo Defienne , Bienvenu Ndagano , Ashley Lyons , Daniele Faccio

Phase reconstruction is important in transmission electron microscopy for structural studies. We describe electron Fourier ptychography and its application to phase reconstruction of both radiation-resistant and beam-sensitive materials. We…

Single particle electron cryomicroscopy (cryo-EM) allows for structures of proteins and protein complexes to be determined from images of non-crystalline specimens. Cryo-EM data analysis requires electron microscope images of randomly…

Instrumentation and Detectors · Physics 2015-11-23 Jianhua Zhao , Marcus A. Brubaker , Samir Benlekbir , John L. Rubinstein

FRET-based approaches are a unique tool for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging and FLIM (Fluorescence Lifetime Imaging Microscopy) enable the visualization of the spatial distribution of…

Quantum correlation, such as entanglement and squeezing have shown to improve phase estimation in interferometric setups on one side, and non-interferometric imaging scheme of amplitude object on the other. In the last case, quantum…

We demonstrate numerical refocusing in coherent confocal laser scanning microscopy based on synthetic optical holography. This physics-based approach implements a computational propagation on the complex signal recovered in synthetic…

This paper discusses new methods for processing images in the photon-limited regime where the number of photons per pixel is binary. We present a new Bayesian denoising method for binary, single-photon images. Each pixel measurement is…

Methodology · Statistics 2016-09-19 Yoann Altmann , Reuben Aspden , Miles Padgett , Steve McLaughlin

The phase retrieval from multi-frequency intensity (power) observations is considered. The object to be reconstructed is complex-valued. A novel algorithm is presented that accomplishes both the object phase (absolute phase) retrieval and…

Signal Processing · Electrical Eng. & Systems 2018-02-07 Vladimir Katkovnik , Karen Egiazarian

Lorentz transmission electron microscopy is a unique characterization technique that enables the simultaneous imaging of both the microstructure and functional properties of materials at high spatial resolution. The quantitative information…

Materials Science · Physics 2020-12-09 Tao Zhou , Mathew Cherukara , Charudatta Phatak

Phase retrieval is the inverse problem of recovering a signal from magnitude-only Fourier measurements, and underlies numerous imaging modalities, such as Coherent Diffraction Imaging (CDI). A variant of this setup, known as holography,…

Machine Learning · Computer Science 2021-04-22 Hannah Lawrence , David A. Barmherzig , Henry Li , Michael Eickenberg , Marylou Gabrié

X-ray near field holography has proven to be a powerful 2D and 3D imaging technique with applications ranging from biomedical research to material sciences. To reconstruct meaningful and quantitative images from the measurement intensities,…

Image and Video Processing · Electrical Eng. & Systems 2024-03-04 Jens Lucht , Leon M. Lohse , Thorsten Hohage , Tim Salditt

Cryogenic electron microscopy (cryo-EM) is an invaluable technique for determining high-resolution three-dimensional structures of biological macromolecules using transmission particle images. The inherent symmetry in these macromolecules…

Optimization and Control · Mathematics 2024-11-06 Qi Zhang , Chenglong Bao , Hai Lin , Mingxu Hu

We propose a new technique for the detection of single atoms in ultracold quantum gases. The technique is based on scanning electron microscopy and employs the electron impact ionization of trapped atoms with a focussed electron probe.…

Statistical Mechanics · Physics 2007-05-23 T. Gericke , C. Utfeld , N. Hommerstad , H. Ott
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