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Identifying complex neural circuitry from electron microscopic (EM) images may help unlock the mysteries of the brain. However, identifying this circuitry requires time-consuming, manual tracing (proofreading) due to the size and intricacy…

Quantitative Methods · Quantitative Biology 2014-09-04 Stephen M. Plaza

Automatic cell image segmentation methods in connectomics produce merge and split errors, which require correction through proofreading. Previous research has identified the visual search for these errors as the bottleneck in interactive…

Computer Vision and Pattern Recognition · Computer Science 2017-04-05 Daniel Haehn , Verena Kaynig , James Tompkin , Jeff W. Lichtman , Hanspeter Pfister

Producing connectomes from electron microscopy (EM) images has historically required a great deal of human proofreading effort. This manual annotation cost is the current bottleneck in scaling EM connectomics, for example, in making larger…

Computer Vision and Pattern Recognition · Computer Science 2025-10-01 Gary B Huang , William M Katz , Stuart Berg , Louis Scheffer

Segmentation of nanoscale electron microscopy (EM) images is crucial but still challenging in connectomics research. One reason for this is that none of the existing segmentation methods are error-free, so they require proofreading, which…

Computer Vision and Pattern Recognition · Computer Science 2022-03-14 Khoa Tuan Nguyen , Ganghee Jang , Tran Anh Tuan , Won-ki Jeong

Reconstructing neuronal circuits at the level of synapses is a central problem in neuroscience and becoming a focus of the emerging field of connectomics. To date, electron microscopy (EM) is the most proven technique for identifying and…

Quantitative Methods · Quantitative Biology 2014-12-05 Stephen M. Plaza , Toufiq Parag , Gary B. Huang , Donald J. Olbris , Mathew A. Saunders , Patricia K. Rivlin

Reconstructing a map of neuronal connectivity is a critical challenge in contemporary neuroscience. Recent advances in high-throughput serial section electron microscopy (EM) have produced massive 3D image volumes of nanoscale brain tissue…

Developing automated and semi-automated solutions for reconstructing wiring diagrams of the brain from electron micrographs is important for advancing the field of connectomics. While the ultimate goal is to generate a graph of neuron…

Computer Vision and Pattern Recognition · Computer Science 2016-08-09 William Gray Roncal , Colin Lea , Akira Baruah , Gregory D. Hager

To date, most discoveries of network subcomponents that implement human-interpretable computations in deep vision models have involved close study of single units and large amounts of human labor. We explore scalable methods for extracting…

Computer Vision and Pattern Recognition · Computer Science 2024-04-23 Achyuta Rajaram , Neil Chowdhury , Antonio Torralba , Jacob Andreas , Sarah Schwettmann

Connectomics is an emerging field in neuroscience that aims to reconstruct the 3-dimensional morphology of neurons from electron microscopy (EM) images. Recent studies have successfully demonstrated the use of convolutional neural networks…

Computer Vision and Pattern Recognition · Computer Science 2017-02-27 Shibani Santurkar , David Budden , Alexander Matveev , Heather Berlin , Hayk Saribekyan , Yaron Meirovitch , Nir Shavit

Extracting a connectome from an electron microscopy (EM) data set requires identification of neurons and determination of synapses between neurons. As manual extraction of this information is very time-consuming, there has been extensive…

Computer Vision and Pattern Recognition · Computer Science 2016-04-12 Gary B. Huang , Louis K. Scheffer , Stephen M. Plaza

One of the critical steps in improving accurate single neuron reconstruction from three-dimensional (3D) optical microscope images is the neuronal structure segmentation. However, they are always hard to segment due to the lack in quality.…

Image and Video Processing · Electrical Eng. & Systems 2021-01-25 Heng Wang , Yang Song , Chaoyi Zhang , Jianhui Yu , Siqi Liu , Hanchuan Peng , Weidong Cai

We develop the first (to the best of our knowledge) provably correct neural networks for a precise computational task, with the proof of correctness generated by an automated verification algorithm without any human input. Prior work on…

Machine Learning · Computer Science 2024-05-09 Rudy Bunel , Krishnamurthy Dvijotham , M. Pawan Kumar , Alessandro De Palma , Robert Stanforth

Automatic segmentation of neuronal topology is critical for handling large scale neuroimaging data, as it can greatly accelerate neuron annotation and analysis. However, the intricate morphology of neuronal branches and the occlusions among…

Image and Video Processing · Electrical Eng. & Systems 2025-08-01 Huayu Fu , Jiamin Li , Haozhi Qu , Xiaolin Hu , Zengcai Guo

The current neuron reconstruction pipeline for electron microscopy (EM) data usually includes automatic image segmentation followed by extensive human expert proofreading. In this work, we aim to reduce human workload by predicting…

Computer Vision and Pattern Recognition · Computer Science 2024-01-09 Qihua Chen , Xuejin Chen , Chenxuan Wang , Yixiong Liu , Zhiwei Xiong , Feng Wu

While Large Vision-Language Models (LVLMs) have demonstrated remarkable capabilities for reasoning and self-correction at the textual level, these strengths provide minimal benefits for complex tasks centered on visual perception, such as…

Computer Vision and Pattern Recognition · Computer Science 2026-02-19 Jinsong Li , Xiaoyi Dong , Yuhang Zang , Yuhang Cao , Jiaqi Wang , Dahua Lin

Shape graphs are complex geometrical structures commonly found in biological and anatomical systems. A shape graph is a collection of nodes, some connected by curvilinear edges with arbitrary shapes. Their high complexity stems from the…

Computation · Statistics 2024-09-17 Benjamin Beaudett , Anuj Srivastava

Neuroscientists are actively pursuing high-precision maps, or graphs, consisting of networks of neurons and connecting synapses in mammalian and non-mammalian brains. Such graphs, when coupled with physiological and behavioral data, are…

While nowadays deep neural networks achieve impressive performances on semantic segmentation tasks, they are usually trained by optimizing pixel-wise losses such as cross-entropy. As a result, the predictions outputted by such networks…

Computer Vision and Pattern Recognition · Computer Science 2019-05-07 Yifu Chen , Arnaud Dapogny , Matthieu Cord

Neural circuits can be reconstructed from brain images acquired by serial section electron microscopy. Image analysis has been performed by manual labor for half a century, and efforts at automation date back almost as far. Convolutional…

Computer Vision and Pattern Recognition · Computer Science 2019-05-01 Kisuk Lee , Nicholas Turner , Thomas Macrina , Jingpeng Wu , Ran Lu , H. Sebastian Seung

We define and study error detection and correction tasks that are useful for 3D reconstruction of neurons from electron microscopic imagery, and for image segmentation more generally. Both tasks take as input the raw image and a binary mask…

Computer Vision and Pattern Recognition · Computer Science 2017-12-05 Jonathan Zung , Ignacio Tartavull , Kisuk Lee , H. Sebastian Seung
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