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Diffraction-limited imaging in epi-fluorescence microscopy remains a challenge when sample aberrations are present or when the region of interest rests deep within an inhomogeneous medium. Adaptive optics is an attractive solution albeit…

Diffraction-limited imaging through complex scattering media is a long sought after goal with important applications in biomedical research. In recent years, high resolution wavefront-shaping has emerged as a powerful approach to generate a…

Optics · Physics 2014-10-17 Ori Katz , Eran Small , Yefeng Guan , Yaron Silberberg

While scattered light conveys most of the information we perceive, scattering may also distort that information before it reaches our detectors. The problem is acute in many applications, such as in high-resolution microscopy of biological…

Adaptive optics (AO) are reconfigurable devices that compensate for wavefront distortions or aberrations in optical systems such as microscopes, telescopes and ophthalmoscopes. Aberrations have detrimental effects that can reduce imaging…

Optics · Physics 2025-06-10 Biwei Zhang , Martin J. Booth , Qi Hu

High-throughput 2D and 3D scanning electron microscopy, which relies on automation and dependable control algorithms, requires high image quality with minimal human intervention. Classical focus and astigmatism correction algorithms attempt…

Instrumentation and Detectors · Physics 2023-05-10 Philipp Johannes Schubert , Rangoli Saxena , Joergen Kornfeld

Thick biological tissues give rise to not only the scattering of incoming light waves, but also aberrations of the remaining unscattered waves. Due to the inability of existing optical imaging methodologies to overcome both of these…

Most camera lens systems are designed in isolation, separately from downstream computer vision methods. Recently, joint optimization approaches that design lenses alongside other components of the image acquisition and processing pipeline…

Computer Vision and Pattern Recognition · Computer Science 2023-03-29 Geoffroi Côté , Fahim Mannan , Simon Thibault , Jean-François Lalonde , Felix Heide

Non-invasive detection of objects embedded inside an optically scattering medium is essential for numerous applications in engineering and sciences. However, in most applications light at visible or near-infrared wavebands is scattered by…

Fluorescence imaging is an essential diagnostic tool in many fields, but diffraction-limited optical imaging at depth is limited by scattering. Here, we present a method based on multiple random illuminations, combined with a computational…

Optics · Physics 2026-03-30 Lei Zhu , Tengfei Wu , Bernhard Rauer , Hilton B. de Aguiar , Sylvain Gigan

Imaging through complex scattering media is severely limited by aberrations and scattering which obscure images and reduce resolution. Confocal and temporal gatings partly filter out multiple scattering but are severely degraded by…

Optics · Physics 2026-05-18 Yiwen Zhang , Minh Dinh , Zeyu Wang , Tianhao Zhang , Tianhang Chen , Chia Wei Hsu

Adaptive optics (AO) is a powerful image correction technique with proven benefits for many life-science microscopy methods. However, the complexity of adding a reflective wavefront modulator and a wavefront sensor into already complicated…

Optical-resolution fluorescence imaging through and within complex samples presents a significant challenge due to random light scattering, with substantial implications across multiple fields. While significant advancements in coherent…

Optics · Physics 2023-12-27 Gil Weinberg , Elad Sunray , Ori Katz

Two-photon (2P) microscopy is a powerful technique for deep-tissue fluorescence imaging; however, tissue scattering limits its effectiveness for depth imaging using conventional approaches. Despite typical strategies having been put forward…

Optics · Physics 2025-05-21 Lei Zhu , Bernhard Rauer , Hilton B. De Aguiar , Sylvain Gigan

Scattering of light in complex media scrambles optical wavefronts and breaks the principles of conventional imaging methods. For decades, researchers have endeavored to conquer the problem by inventing approaches such as adaptive optics,…

Focusing waves inside inhomogeneous media is a fundamental problem for imaging. Spatial variations of wave velocity can strongly distort propagating wavefronts and degrade image quality. Adaptive focusing can compensate for such aberration,…

Image and Video Processing · Electrical Eng. & Systems 2020-06-15 William Lambert , Laura A. Cobus , Thomas Frappart , Mathias Fink , Alexandre Aubry

We revisit the problem of model-based object recognition for intensity images and attempt to address some of the shortcomings of existing Bayesian methods, such as unsuitable priors and the treatment of residuals with a non-robust error…

Computer Vision and Pattern Recognition · Computer Science 2010-12-14 Vasileios Zografos , Bernard Buxton

Machine learning methods for computational imaging require uncertainty estimation to be reliable in real settings. While Bayesian models offer a computationally tractable way of recovering uncertainty, they need large data volumes to be…

Machine Learning · Computer Science 2020-08-24 Francesco Tonolini , Jack Radford , Alex Turpin , Daniele Faccio , Roderick Murray-Smith

Ultrathin meta-optics offer unmatched, multifunctional control of light. Next-generation optical technologies, however, demand unprecedented performance. This will likely require design algorithms surpassing the capability of human…

Optics · Physics 2021-04-06 Shane Colburn , Arka Majumdar

In biological microscopy, light scattering represents the main limitation to image at depth. Recently, a set of wavefront shaping techniques has been developed in order to manipulate coherent light in strongly disordered materials. The…

Optics · Physics 2021-01-07 Antoine Boniface , Jonathan Dong , Sylvain Gigan

Adaptive optics enables the deployment of interferometer-based spectroscopy without the need for moving parts necessary for scanning the interferometer arms. Here, we employ a Michelson Interferometer in conjunction with a Spatial Light…

Optics · Physics 2025-12-05 Jesneil Lauren Lewis , Ayan Banerjee