Related papers: Differentiable model-based adaptive optics for two…
Aberrations limit optical systems in many situations, for example when imaging in biological tissue. Machine learning offers novel ways to improve imaging under such conditions by learning inverse models of aberrations. Learning requires…
Light scattering and aberrations limit optical microscopy in biological tissue, which motivates the development of adaptive optics techniques. Here, we develop a method for adaptive optics with reflected light and deep neural networks…
Adaptive optics in combination with multi-photon techniques is a powerful approach to image deep into a specimen. Remarkably, virtually all adaptive optics schemes today rely on wavefront modulators which are reflective, diffractive, or…
Inhomogeneities in the refractive index of a biological sample can introduce phase aberrationsin microscopy systems, severely impairing the quality of images. Adaptive optics can be employed to correct for phase aberrations and improve…
Images obtained by microscopes are generally degraded by aberrations. Adaptive optics (AO) has been widely used in to compensate for aberrations and improve reduced image quality. Requiring no separate wavefront sensor, sensorless AO…
Diffraction-limited focusing deep into biological tissue is challenging due to spherical aberrations that lead to a broadening of the focal spot particularly in axial direction. While the diffraction-limit can be restored employing…
Optical aberrations prevent telescopes from reaching their theoretical diffraction limit. Once estimated, these aberrations can be compensated for using deformable mirrors in a closed loop. Focal plane wavefront sensing enables the…
We describe an adaptive optics technique for two-photon microscopy in which the deformable mirror used for aberration compensation is positioned in a plane conjugate to the plane of the aberration. We demonstrate in a proof-of-principle…
Adaptive optics is a strategy to compensate for sample-induced aberrations in microscopy applications. Generally, it requires the presence of "guide stars" in the sample to serve as localized reference targets. We describe an implementation…
Estimation of optical aberrations from volumetric intensity images is a key step in sensorless adaptive optics for 3D microscopy. Recent approaches based on deep learning promise accurate results at fast processing speeds. However,…
Control of light through a microscope objective with a high numerical aperture is a common requirement in applications such as optogenetics, adaptive optics, or laser processing. Light propagation, including polarization effects, can be…
Adaptive optics (AO) has revolutionized imaging in {fields} from astronomy to microscopy by correcting optical aberrations. In label-free microscopes, however, conventional AO faces limitations due to the absence of guidestar and the need…
Optical focusing through/inside scattering media, like multimode fiber and biological tissues, has significant impact in biomedicine yet considered challenging due to strong scattering nature of light. Previously, promising progress has…
Adaptive optics can focus light through opaque media by compensating the random phase delay acquired while crossing a scattering curtain. The technique is commonly exploited in many fields, including astrophysics, microscopy, biomedicine…
Microscope objectives achieve near diffraction-limited performance only when used under the conditions they are designed for. In non-standard geometries, such as thick cover slips or curved surfaces, severe aberrations arise, inevitably…
Fluorescence Correlation Spectroscopy (FCS) yields measurement parameters (number of molecules, diffusion time) that characterize the concentration and kinetics of fluorescent molecules within a supposedly known observation volume. Absolute…
In optical imaging, light propagation is affected by the inhomogeneities of the medium. Sample-induced aberrations and multiple scattering can strongly degrade the image resolution and contrast. Based on a dynamic correction of the incident…
Optical approaches to fluorescent, spectroscopic, and morphological imaging have made exceptional advances in the last decade. Super-resolution imaging and wide-field multiphoton imaging are now underpinning major advances across the…
Microscopy and optical imaging are drastically limited by the inhomogeneities encountered by the light while propagating from the object of interest to the detection system. In this context, adaptive optics and wavefront manipulation are…
Confocal Raman microscopy, a highly specific and label-free technique for the microscale study of thick samples, often presents difficulties due to weak Raman signals. Inhomogeneous samples introduce wavefront aberrations that further…