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Many experiments in biological and medical sciences currently use multiphoton microscopy as a core imaging technique. To date, solid-state lasers are most commonly used as excitation beam sources. However, the most demanding applications…

Low-light optical imaging refers to the use of cameras to capture images with minimal photon flux. This area has broad application to diverse fields, including optical microscopy for biological studies. In such studies, it is important to…

Fluorescence microscopy has revolutionized biomedical research over the past three decades. Its high molecular specificity and unrivaled single molecule level sensitivity have enabled breakthroughs in a variety of research fields. For in…

Optics · Physics 2012-03-21 Meng Cui , Ke Si , Reto Fiolka

Real-time monitoring of dynamic biological processes in the body is critical to understanding disease progression and treatment response. This data, for instance, can help address the lower than 50% response rates to cancer immunotherapy.…

Recently, it was discovered that microsphere can generate super-resolution focusing beyond diffraction limit. This has led to the development of an exciting super-resolution imaging technique -microsphere nanoscopy- that features a record…

Optics · Physics 2021-08-26 Zengbo Wang

The progress of semiconductor electronics toward ever-smaller length scales and associated higher power densities brings a need for new high-resolution thermal microscopy techniques. Traditional thermal microscopy is performed by detecting…

Materials Science · Physics 2015-05-13 Todd Brintlinger , Yi Qi , Kamal H. Baloch , David Goldhaber-Gordon , John Cumings

We have used a photothermal technique, in which chopped light heats the front surface of a small ( ~ 1 mm2) sample and the chopping frequency dependence of thermal radiation from the back surface is measured with a liquid nitrogen cooled…

Materials Science · Physics 2016-01-20 J. W. Brill , Maryam Shahi , Marcia M. Payne , Jesper Edberg , Y. Yao , Xavier Crispin , J. E. Anthony

Mid-infrared (MIR) spectroscopy is a powerful technique employed for a variety of applications, including gas sensing, industrial inspection, astronomy, surveillance, and imaging. Thin-film narrowband interference filters, targeted to…

Fluorescence imaging is indispensable to biology and neuroscience. The need for large-scale imaging in freely behaving animals has further driven the development in miniaturized microscopes (miniscopes). However, conventional microscopes /…

Optics · Physics 2020-10-29 Yujia Xue , Ian G. Davison , David A. Boas , Lei Tian

Mid-infrared (MIR) spectral imaging enables precise target identification and analysis by capturing rich chemical fingerprints, which calls for high-sensitivity broadband MIR imagers at room temperature. Here, we devise and implement a…

Optics · Physics 2026-05-26 Yue Song , Jianan Fang , Wen Zhang , Yijing Li , Ben Sun , Zhiwei Jia , Kun Huang , Heping Zeng

As photonic technologies continue to grow in multidimensional aspects, integrated photonics holds a unique position and continuously presents enormous possibilities to research communities. Applications span across data centers,…

Multi-photon microscopy has played a significant role in biological imaging since it allows to observe living tissues with improved penetration depth and excellent sectioning effect. Multi-photon microscopy relies on multi-photon…

Fourier ptychographic (FP) microscope is a coherent imaging method that can synthesize an image with a higher bandwidth using multiple low-bandwidth images captured at different spatial frequency regions. The method's demand for multiple…

Optics · Physics 2016-03-25 Jaebum Chung , Hangwen Lu , Xiaoze Ou , Haojiang Zhou , Changhuei Yang

On-invasive optical imaging techniques are essential diagnostic tools in many fields. Although various recent methods have been proposed to utilize and control light in multiple scattering media, non-invasive optical imaging through and…

Fourier-transform infrared spectroscopy (FTIR) is an indispensable analytical method that allows label-free identification of substances via fundamental molecular vibrations. However, the sensitivity of FTIR is often limited by the low…

High-speed mid-infrared (MIR) videography constitutes an enabling tool to monitor and analyze various dynamics in scientific research and industrial applications, such as combustion diagnostics, explosion reactions, photosynthetic tracking,…

Optics · Physics 2026-05-28 Ruiyang Qin , Kun Huang , Min Peng , Jianan Fang , Ben Sun , Zhengru Guo , Heping Zeng

Applications relying on mid-infrared radiation (Mid-IR, $\lambda\sim$ 3-30 $\mu$m) have progressed at a very rapid pace in recent years, stimulated by scientific and technological breakthroughs. Mid-IR cameras have propelled the field of…

We report on mid-infrared imaging of hot cores performed with SpectroCam-10 and TIMMI2. The observations aimed at the detection of thermal emission presumably associated with the hot cores. Mid-infrared flux measurements are required to…

Astrophysics · Physics 2017-04-12 B. Stecklum , B. Brandl , M. Feldt , Th. Henning , H. Linz , I. Pascucci

The penetration depth of photoacoustic imaging in biological tissues has been fundamentally limited by the strong optical attenuation when light is delivered externally through the tissue surface. To address this issue, we previously…

Medical Physics · Physics 2021-02-24 Mucong Li , Tri Vu , Georgy Sankii , Brenton Winship , Kohldon Boydston , Russell Terry , Pei Zhong , Junjie Yao

Low quantum yields of near infrared (NIR) fluorophores have limited their capabilities as imaging probes in a transparent, low background imaging window. Here for the first time we reported near-infrared fluorescence enhance (NIR-FE) cell…

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