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Generalization is one of the main challenges of computational pathology. Slide preparation heterogeneity and the diversity of scanners lead to poor model performance when used on data from medical centers not seen during training. In order…

Image and Video Processing · Electrical Eng. & Systems 2024-01-09 Nicolas Nerrienet , Rémy Peyret , Marie Sockeel , Stéphane Sockeel

Deep learning models that are trained on histopathological images obtained from a single lab and/or scanner give poor inference performance on images obtained from another scanner/lab with a different staining protocol. In recent years,…

Image and Video Processing · Electrical Eng. & Systems 2020-10-07 Harshal Nishar , Nikhil Chavanke , Nitin Singhal

Imaging mass spectrometry (IMS) is a powerful tool for untargeted, highly multiplexed molecular mapping of tissue in biomedical research. IMS offers a means of mapping the spatial distributions of molecular species in biological tissue with…

Computer Vision and Pattern Recognition · Computer Science 2025-08-05 Yijie Zhang , Luzhe Huang , Nir Pillar , Yuzhu Li , Lukasz G. Migas , Raf Van de Plas , Jeffrey M. Spraggins , Aydogan Ozcan

Variability in staining protocols, such as different slide preparation techniques, chemicals, and scanner configurations, can result in a diverse set of whole slide images (WSIs). This distribution shift can negatively impact the…

Image and Video Processing · Electrical Eng. & Systems 2023-04-25 Kudaibergen Abutalip , Numan Saeed , Mustaqeem Khan , Abdulmotaleb El Saddik

Virtual stain transfer is a promising area of research in Computational Pathology, which has a great potential to alleviate important limitations when applying deeplearningbased solutions such as lack of annotations and sensitivity to a…

Computer Vision and Pattern Recognition · Computer Science 2022-10-19 Jelica Vasiljević , Friedrich Feuerhake , Cédric Wemmert , Thomas Lampert

Biomedical images are noisy. The imaging equipment itself has physical limitations, and the consequent experimental trade-offs between signal-to-noise ratio, acquisition speed, and imaging depth exacerbate the problem. Denoising is,…

Image and Video Processing · Electrical Eng. & Systems 2020-11-11 Mikhail Papkov , Kenny Roberts , Lee Ann Madissoon , Omer Bayraktar , Dmytro Fishman , Kaupo Palo , Leopold Parts

Stain variation across hospitals degrades histopathology models at deployment. Existing augmentation methods perturb color spaces with arbitrary hyperparameters, lacking both a principled budget and coverage guarantees for unseen centers.…

Image and Video Processing · Electrical Eng. & Systems 2026-05-15 Mingi Hong

Stain variations often decrease the generalization ability of deep learning based approaches in digital histopathology analysis. Two separate proposals, namely stain normalization (SN) and stain augmentation (SA), have been spotlighted to…

Computer Vision and Pattern Recognition · Computer Science 2023-03-03 Yiqing Shen , Yulin Luo , Dinggang Shen , Jing Ke

Compared to hematoxylin-eosin (H&E) staining, immunohistochemistry (IHC) not only maintains the structural features of tissue samples, but also provides high-resolution protein localization, which is essential for aiding in pathology…

Computer Vision and Pattern Recognition · Computer Science 2025-11-04 Yuhang Kang , Ziyu Su , Tianyang Wang , Zaibo Li , Wei Chen , Muhammad Khalid Khan Niazi

Foundation models trained with self-supervised learning (SSL) on large-scale histological images have significantly accelerated the development of computational pathology. These models can serve as backbones for region-of-interest (ROI)…

Computer Vision and Pattern Recognition · Computer Science 2026-02-05 Jiawen Li , Jiali Hu , Xitong Ling , Yongqiang Lv , Yuxuan Chen , Yizhi Wang , Tian Guan , Yifei Liu , Yonghong He

The presence and density of specific types of immune cells are important to understand a patient's immune response to cancer. However, immunofluorescence staining required to identify T cell subtypes is expensive, time-consuming, and rarely…

Computer Vision and Pattern Recognition · Computer Science 2022-10-18 Georg Wölflein , In Hwa Um , David J Harrison , Ognjen Arandjelović

Hyperspectral imaging has been widely used for spectral and spatial identification of target molecules, yet often contaminated by sophisticated noise. Current denoising methods generally rely on independent and identically distributed noise…

Image and Video Processing · Electrical Eng. & Systems 2024-09-17 Guangrui Ding , Chang Liu , Jiaze Yin , Xinyan Teng , Yuying Tan , Hongjian He , Haonan Lin , Lei Tian , Ji-Xin Cheng

Traditional staining normalization approaches, e.g. Macenko, typically rely on the choice of a single representative reference image, which may not adequately account for the diverse staining patterns of datasets collected in practical…

Image and Video Processing · Electrical Eng. & Systems 2024-06-11 Desislav Ivanov , Carlo Alberto Barbano , Marco Grangetto

Segmentation from renal pathological images is a key step in automatic analyzing the renal histological characteristics. However, the performance of models varies significantly in different types of stained datasets due to the appearance…

Image and Video Processing · Electrical Eng. & Systems 2020-02-21 Ke Mei , Chuang Zhu , Lei Jiang , Jun Liu , Yuanyuan Qiao

Computational histopathology image diagnosis becomes increasingly popular and important, where images are segmented or classified for disease diagnosis by computers. While pathologists do not struggle with color variations in slides,…

Image and Video Processing · Electrical Eng. & Systems 2020-07-27 Hanwen Liang , Konstantinos N. Plataniotis , Xingyu Li

Generative adversarial networks using a cycle-consistency loss facilitate unpaired training of image-translation models and thereby exhibit a very high potential in manifold medical applications. However, the fact that images in one domain…

Image and Video Processing · Electrical Eng. & Systems 2021-01-12 Michael Gadermayr , Maximilian Tschuchnig , Laxmi Gupta , Dorit Merhof , Nils Krämer , Daniel Truhn , Burkhard Gess

Hematoxylin and eosin (H&E)-stained slides are central to cancer diagnosis and monitoring, visualizing tissue architecture and cellular morphology. However, H&E lacks the molecular specificity needed to distinguish cell states and…

Image and Video Processing · Electrical Eng. & Systems 2026-04-14 Tushar Kataria , Beatrice Knudsen , Shireen Y. Elhabian

Modern histopathology relies on the microscopic examination of thin tissue sections stained with histochemical techniques, typically using brightfield or fluorescence microscopy. However, the staining of samples can permanently alter their…

Breast cancer presents a significant healthcare challenge globally, demanding precise diagnostics and effective treatment strategies, where histopathological examination of Hematoxylin and Eosin (H&E) stained tissue sections plays a central…

Image and Video Processing · Electrical Eng. & Systems 2024-08-06 Linhao Qu , Chengsheng Zhang , Guihui Li , Haiyong Zheng , Chen Peng , Wei He

Immunohistochemistry is a valuable diagnostic tool for cancer pathology. However, it requires specialist labs and equipment, is time-intensive, and is difficult to reproduce. Consequently, a long term aim is to provide a digital method of…

Image and Video Processing · Electrical Eng. & Systems 2022-04-15 Christopher D. Walsh , Joanne Edwards , Robert H. Insall