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Over the last two decades, spatial light modulators (SLMs) have revolutionised our ability to shape optical fields. They grant independent dynamic control over thousands of degrees-of-freedom within a single light beam. In this work we test…

Sub-micrometer scale light patterns play a pivotal role in various fields, including biology, biophysics, and AMO physics. High-resolution, in situ observation of light profiles is essential for their design and application. However,…

Multifocal microscopy (MFM) offers high-speed three-dimensional imaging through the simultaneous image capture from multiple focal planes. Conventional MFM systems use a fabricated grating in the emission path for a single emission…

Biological Physics · Physics 2019-07-31 M. Junaid Amin , Sabine Petry , Haw Yang , Joshua W. Shaevitz

Recent advancements in diffusion models (DMs) have greatly advanced remote sensing image super-resolution (RSISR). However, their iterative sampling processes often result in slow inference speeds, limiting their application in real-time…

Image and Video Processing · Electrical Eng. & Systems 2025-03-26 Xiaohui Sun , Jiangwei Mo , Hanlin Wu , Jie Ma

Molecule-surface interaction is key to many physical and chemical processes at interfaces. Here, we show that the dynamics of single molecules on a surface under ultrahigh vacuum can be resolved using fluorescence imaging. By adapting…

Chemical Physics · Physics 2021-10-19 Tianyu Fang , Florian Elsen , Nick Vogeley , Daqing Wang

We present a novel experimental setup in which magnetic and optical tweezers are combined for torque and force transduction onto single filamentous molecules in a transverse configuration to allow simultaneous mechanical measurement and…

Biomolecules · Quantitative Biology 2015-06-24 Zhaokun Zhou , Helen Miller , Adam J. M. Wollman , Mark C. Leake

Digital micromirror device (DMD) serves in a significant part of computational optical setups as a means of encoding an image by the desired pattern. The most prominent is its usage in the so-called single-pixel camera experiment. This…

Instrumentation and Detectors · Physics 2020-06-24 Lukas Klein , Karel Zidek

Precisely measuring the three-dimensional position and orientation of individual fluorophores is challenging due to the substantial photon shot noise in single-molecule experiments. Facing this limited photon budget, numerous techniques…

Optics · Physics 2021-02-02 Oumeng Zhang , Matthew D. Lew

We report a new coherent imaging technique, termed ptychographic structured modulation (PSM), for quantitative super-resolution microscopy. In this technique, we place a thin diffuser (i.e., a scattering lens) in between the sample and the…

Image and Video Processing · Electrical Eng. & Systems 2019-07-26 Pengming Song , Shaowei Jiang , He Zhang , Zichao Bian , Chengfei Guo , Kazunori Hoshino , Guoan Zheng

Single-pixel imaging has emerged as a key technique in fluorescence microscopy, where fast acquisition and reconstruction are crucial. In this context, images are reconstructed from linearly compressed measurements. In practice, total…

Image and Video Processing · Electrical Eng. & Systems 2025-07-28 Serban C. Tudosie , Valerio Gandolfi , Shivaprasad Varakkoth , Andrea Farina , Cosimo D'Andrea , Simon Arridge

Super-resolution fluorescence microscopy is of great interest in life science studies for visualizing subcellular structures at the nanometer scale. Among various kinds of super-resolution approaches, image scanning microscopy (ISM) offers…

Light field (LF) image super-resolution (SR) is a challenging problem due to its inherent ill-posed nature, where a single low-resolution (LR) input LF image can correspond to multiple potential super-resolved outcomes. Despite this…

Image and Video Processing · Electrical Eng. & Systems 2023-11-29 Wentao Chao , Fuqing Duan , Xuechun Wang , Yingqian Wang , Guanghui Wang

Imaging of scenes using light or other wave phenomena is subject to the diffraction limit. The spatial profile of a wave propagating between a scene and the imaging system is distorted by diffraction resulting in a loss of resolution that…

Image and Video Processing · Electrical Eng. & Systems 2020-07-20 Ji Hyun Nam , Andreas Velten

Fluorescence imaging is an essential diagnostic tool in many fields, but diffraction-limited optical imaging at depth is limited by scattering. Here, we present a method based on multiple random illuminations, combined with a computational…

Optics · Physics 2026-03-30 Lei Zhu , Tengfei Wu , Bernhard Rauer , Hilton B. de Aguiar , Sylvain Gigan

We have developed a light scattering technique based on differential measurement and polarization (differential light scattering, DLS) capable in principle of retrieving timing information with picosecond resolution without the need for…

chao-dyn · Physics 2009-10-31 G. Vacca , R. D. Morgan , R. B. Laughlin

To overcome the limit of diffraction while achieving the superresolution technique, solid immersion lenses are the key optical elements for data storage and nanophotonics applications. Recent demonstrations have shown how different…

Optics · Physics 2026-02-24 Subhankar Roy , Jianping Hu , M Ummal Momeen

With the advent of microsphere assisted microscopy in 2011, this technique emerged as a simple and easy way to obtain optical super-resolution. Although the possible mechanisms of imaging by microspheres are debated in the literature, most…

Optics · Physics 2015-08-27 Navid Farahi

Image deblurring aims to restore the detailed texture information or structures from blurry images, which has become an indispensable step in many computer vision tasks. Although various methods have been proposed to deal with the image…

Computer Vision and Pattern Recognition · Computer Science 2024-10-28 Yanni Zhang , Qiang Li , Miao Qi , Di Liu , Jun Kong , Jianzhong Wang

Recent technological advances in cutting-edge ultrasensitive fluorescence microscopy have allowed single-molecule imaging experiments in living cells across all three domains of life to become commonplace. Single-molecule live-cell data is…

Biomolecules · Quantitative Biology 2015-04-15 Mark Leake

Fluorescence lifetime imaging microscopy (FLIM) is an important technique to understand the chemical micro-environment in cells and tissues since it provides additional contrast compared to conventional fluorescence imaging. When two…

Image and Video Processing · Electrical Eng. & Systems 2022-01-19 Varun Mannam , Xiaotong Yuan , Scott Howard