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Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique in biomedical research that uses the fluorophore decay rate to provide additional contrast in fluorescence microscopy. However, at present, the calculation, analysis,…

Image and Video Processing · Electrical Eng. & Systems 2021-06-09 Varun Mannam , Yide Zhang , Xiaotong Yuan , Cara Ravasio , Scott S. Howard

Fluorescence microscopy is indispensable in nanoscience and biological sciences. The versatility of labeling target structures with fluorescent dyes permits to visualize structure and function at a subcellular resolution with a wide field…

Structured illumination microscopy (SIM) is an important super-resolution based microscopy technique that breaks the diffraction limit and enhances optical microscopy systems. With the development of biology and medical engineering, there…

Image and Video Processing · Electrical Eng. & Systems 2021-11-18 Xi Cheng , Jun Li , Qiang Dai , Zhenyong Fu , Jian Yang

State space models (SSMs) have recently achieved strong performance on long sequence modeling tasks while offering improved memory and computational efficiency compared to transformer based architectures. However, their evaluation has been…

Machine Learning · Computer Science 2026-03-13 Fatemeh Valeh , Monika Farsang , Radu Grosu , Gerhard Schütz

Super-resolution effects in optical and fluorescence biological systems are analyzed and their relations with uncertainty relations are discussed. Super-resolutions obtained in the optical systems, including especially NSOM, SIM and…

Optics · Physics 2015-06-12 Yacob Ben-Aryeh

We exploit memory effect speckle correlations for the imaging of incoherent linear (single-photon) fluorescent sources behind scattering tissue. While memory effect-based imaging techniques have been heavily studied in the past, for thick…

In single-molecule microscopy it is necessary to locate with high precision point sources from noisy observations of the spectrum of the signal at frequencies capped by $f_c$, which is just about the frequency of natural light. This paper…

Information Theory · Computer Science 2015-04-06 Veniamin I. Morgenshtern , Emmanuel J. Candes

Image Phase Alignment Super-Sampling (ImPASS) is a computational imaging algorithm for converting a sequence of displaced low-resolution images into a single high-resolution image. The method consists of a unique combination of Phase…

Optics · Physics 2025-09-01 James N. Caron

Far-field characterization of small objects is severely constrained by the diffraction limit. Existing tools achieving sub-diffraction resolution often utilize point-by-point image reconstruction via scanning or labelling. Here, we present…

Motivated by the importance of optical microscopes to science and engineering, scientists have pondered for centuries how to improve their resolution and the existence of fundamental resolution limits. In recent years, a new class of…

Quantum Physics · Physics 2015-07-28 Mankei Tsang

The short and intense pulses of the new X-ray free electron lasers, now operational or under construction, may make possible diffraction experiments on single molecule-sized objects with high resolution, before radiation damage destroys the…

Data Analysis, Statistics and Probability · Physics 2012-04-11 Miklós Tegze , Gábor Bortel

MINFLUX is an emerging super-resolution technology that measures the position of single fluorophores with nanometer precision using fewer photons than any other fluorescence imaging or tracking technique. Here, we derive simple and…

Optics · Physics 2025-01-09 Zach Marin , Jonas Ries

Following recent advancements in multimode fiber (MMF), miniaturization of imaging endoscopes has proven crucial for minimally invasive surgery in vivo. Recent progress enabled by super-resolution imaging methods with a data-driven deep…

Optics · Physics 2023-07-12 Chuncheng Zhang , Tingting Liu , Zhihua Xie , Yu Wang , Tong Liu , Qian Chen , Xiubao Sui

Super-resolution fluorescence microscopy overcomes blurring arising from light diffraction, allowing the reconstruction of fine scale details in biological structures. Standard methods come at the expense of long acquisition time and/or…

Optimization and Control · Mathematics 2021-10-12 Bastien Laville , Laure Blanc-Féraud , Gilles Aubert

Single molecule imaging is one of the main target areas of X-ray free electron lasers. It relies on the possibility of orienting the large number of low counting statistics 2D diffraction patterns taken at random orientations of identical…

Atomic and Molecular Clusters · Physics 2012-11-30 Zoltan Jurek , Gyula Faigel

In recent years several far-field microscopy techniques have been developed which manage to overcome the diffraction limit of resolution. A unifying classification scheme for them is clearly desirable. We argue that existing schemes based…

Optics · Physics 2016-06-17 Oliver Passon , Johannes Grebe-Ellis

In the ELTs era, where the need for versatile and innovative solutions to produce very high spatial resolution images has become a major issue, the search of synergies with other science fields seems a logic step. One of the considered…

Instrumentation and Methods for Astrophysics · Physics 2018-11-15 E. Quevedo , S. Velasco , C. Colodro-Conde , G. Rodríguez-Coira , A. Oscoz , R. L. López , J. Font , J. H. Brito , O. Llinas , S. Robaina , G. M. Callico , R. Sarmiento , R. Rebolo

The photo-kinetics of fluorescent molecules have enabled the circumvention of far-field optical diffraction-limit. Despite its enormous potential, the necessity to label the sample may adversely influence the delicate biology under…

Fluorescence lifetime imaging microscopy (FLIM) is a powerful quantitative technique that provides metabolic and molecular contrast, offering strong translational potential for label-free, real-time diagnostics. However, its clinical…

Computer Vision and Pattern Recognition · Computer Science 2025-12-19 Paloma Casteleiro Costa , Parnian Ghapandar Kashani , Xuhui Liu , Alexander Chen , Ary Portes , Julien Bec , Laura Marcu , Aydogan Ozcan

Single particle imaging (SPI) at X-ray free electron lasers (XFELs) is a technique to determine the 3D structure of nanoscale objects like biomolecules from a large number of diffraction patterns of copies of these objects in random…

Image and Video Processing · Electrical Eng. & Systems 2022-10-20 Abhishek Mall , Kartik Ayyer
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