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Super-resolution microscopy overcomes the diffraction limit of conventional light microscopy in spatial resolution. By providing novel spatial or spatio-temporal information on biological processes at nanometer resolution with molecular…

Biological Physics · Physics 2021-11-10 Tianjie Yang , Yaoru Luo , Wei Ji , Ge Yang

Volumetric imaging by fluorescence microscopy is often limited by anisotropic spatial resolution from inferior axial resolution compared to the lateral resolution. To address this problem, here we present a deep-learning-enabled…

Computer Vision and Pattern Recognition · Computer Science 2022-07-06 Hyoungjun Park , Myeongsu Na , Bumju Kim , Soohyun Park , Ki Hean Kim , Sunghoe Chang , Jong Chul Ye

Surveillance scenarios are prone to several problems since they usually involve low-resolution footage, and there is no control of how far the subjects may be from the camera in the first place. This situation is suitable for the…

Computer Vision and Pattern Recognition · Computer Science 2021-01-27 Angelo G. Menezes

Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization…

Super-resolution fluorescence microscopy, with a resolution beyond the diffraction limit of light, has become an indispensable tool to directly visualize biological structures in living cells at a nanometer-scale resolution. Despite…

Computer Vision and Pattern Recognition · Computer Science 2018-09-05 Yu Li , Fan Xu , Fa Zhang , Pingyong Xu , Mingshu Zhang , Ming Fan , Lihua Li , Xin Gao , Renmin Han

Single-molecule localization fluorescence microscopy constructs super-resolution images by sequential imaging and computational localization of sparsely activated fluorophores. Accurate and efficient fluorophore localization algorithms are…

Image and Video Processing · Electrical Eng. & Systems 2020-07-21 Artur Speiser , Lucas-Raphael Müller , Ulf Matti , Christopher J. Obara , Wesley R. Legant , Jonas Ries , Jakob H. Macke , Srinivas C. Turaga

Far-field characterization of small objects is severely constrained by the diffraction limit. Existing tools achieving sub-diffraction resolution often utilize point-by-point image reconstruction via scanning or labelling. Here, we present…

Super-resolution reconstruction techniques entail the utilization of software algorithms to transform one or more sets of low-resolution images captured from the same scene into high-resolution images. In recent years, considerable…

Computer Vision and Pattern Recognition · Computer Science 2024-08-02 Hao Yan , Zixiang Wang , Zhengjia Xu , Zhuoyue Wang , Zhizhong Wu , Ranran Lyu

Intense short-wavelength pulses from free-electron lasers and high-harmonic-generation sources enable diffractive imaging of individual nano-sized objects with a single x-ray laser shot. The enormous data sets with up to several million…

Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution…

Biological Physics · Physics 2007-08-27 James H. Rice

Traditional fluorescence microscopy is constrained by inherent trade-offs among resolution, field-of-view, and system complexity. To navigate these challenges, we introduce a simple and low-cost computational multi-aperture miniature…

Optics · Physics 2024-05-31 Qianwan Yang , Ruipeng Guo , Guorong Hu , Yujia Xue , Yunzhe Li , Lei Tian

Deep learning-based super-resolution models have the potential to revolutionize biomedical imaging and diagnoses by effectively tackling various challenges associated with early detection, personalized medicine, and clinical automation.…

Medical Physics · Physics 2023-06-27 Yuanzheng Ma , Xinyue Wang , Benqi Zhao , Ying Xiao , Shijie Deng , Jian Song , Xun Guan

Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the universal…

Image super-resolution technology is the process of obtaining high-resolution images from one or more low-resolution images. With the development of deep learning, image super-resolution technology based on deep learning method is emerging.…

Computer Vision and Pattern Recognition · Computer Science 2022-01-26 Fangyuan Zhu

Depth image super-resolution is an extremely challenging task due to the information loss in sub-sampling. Deep convolutional neural network have been widely applied to color image super-resolution. Quite surprisingly, this success has not…

Computer Vision and Pattern Recognition · Computer Science 2016-07-08 Xibin Song , Yuchao Dai , Xueying Qin

In example-based super-resolution, the function relating low-resolution images to their high-resolution counterparts is learned from a given dataset. This data-driven approach to solving the inverse problem of increasing image resolution…

Image and Video Processing · Electrical Eng. & Systems 2018-12-05 Alexander Robey , Vidya Ganapati

Fluorescence lifetime imaging microscopy (FLIM) is a powerful quantitative technique that provides metabolic and molecular contrast, offering strong translational potential for label-free, real-time diagnostics. However, its clinical…

Computer Vision and Pattern Recognition · Computer Science 2025-12-19 Paloma Casteleiro Costa , Parnian Ghapandar Kashani , Xuhui Liu , Alexander Chen , Ary Portes , Julien Bec , Laura Marcu , Aydogan Ozcan

Fluorescence microscopy is essential to study biological structures and dynamics. However, existing systems suffer from a tradeoff between field-of-view (FOV), resolution, and complexity, and thus cannot fulfill the emerging need of…

Optics · Physics 2022-09-09 Yujia Xue , Qianwan Yang , Guorong Hu , Kehan Guo , Lei Tian

The use of fluorescent molecules to create long sequences of low-density, diffraction-limited images enables highly-precise molecule localization. However, this methodology requires lengthy imaging times, which limits the ability to view…

Image and Video Processing · Electrical Eng. & Systems 2024-03-26 Yair Ben Sahel , Yonina C. Eldar

Recently, deep learning approaches have become the main research frontier for biological image reconstruction and enhancement problems thanks to their high performance, along with their ultra-fast inference times. However, due to the…

Image and Video Processing · Electrical Eng. & Systems 2022-03-18 Mehmet Akçakaya , Burhaneddin Yaman , Hyungjin Chung , Jong Chul Ye
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