Related papers: Low Stress Ion Conductance Microscopy of Sub-Cellu…
Cell membranes separate the cell interior from the external environment. They are constituted by a variety of lipids; their composition determines the dynamics of membrane proteins and affects the ability of the cells to adapt. Even though…
Patch-clamp technique is the gold standard for cellular electrophysiological measurements, which is capable of measuring single ion transport events across the cell membrane. However, the measurement possesses significant complexity, and it…
Animal cells in tissues are supported by biopolymer matrices, which typically exhibit highly nonlinear mechanical properties. While the linear elasticity of the matrix can significantly impact cell mechanics and functionality, it remains…
The mechanical properties of PC12 living cells have been studied at the nanoscale with a Force Feedback Microscope using two experimental approaches. Firstly, the local mechanical impedance of the cell membrane has been mapped…
The electrical properties of extracellular space around neurons are important to understand the genesis of extracellular potentials, as well as for localizing neuronal activity from extracellular recordings. However, the exact nature of…
Precise control of tensile stress and intrinsic damping is crucial for the optimal design of nanomechanical systems for sensor applications and quantum optomechanics in particular. In this letter we study the in uence of oxygen plasma on…
Plasma impedance probes (PIPs) are a type of RF probe that primarily measure electron density. This work introduces two advancements: a streamlined analytical model for interpreting PIP-monopole measurements and techniques for achieving…
The effect of AC electric fields on the elasticity of supported lipid bilayers has been investigated at the microscopic level using grazing incidence synchrotron x-ray scattering. A strong decrease in the membrane tension up to 1mN/m and a…
Response of lipid bilayers to external mechanical stimuli is an active area of research with implications for fundamental and synthetic cell biology. However, there is a lack of tools for systematically imposing mechanical strains and…
We have developed a novel scanning probe-based methodology to study cell biomechanics. The time dependence of the force exerted by the cell surface on a scanning probe at constant local deformation has been used to extract local…
We develop and thoroughly test a stress-controlled, parallel plates shear cell that can be coupled to an optical microscope or a small angle light scattering setup, for simultaneous investigation of the rheological properties and the…
A plasma impedance probe (PIP) is a type of in-situ, radio-frequency (RF) probe that is traditionally used to measure plasma properties (e.g. density) in low-density environments such as the Earth's ionosphere. We believe that PIPs are…
Recent advances in optical technology have significantly enhanced the resolution of imaging of living cells, achieving nanometer-scale precision. However, the crowded three-dimensional environment within cells presents a challenge for…
The study of cells' dynamical properties is essential to a better understanding of several physiological processes. These properties are directly associated with cells' mechanical parameters experimentally achieved through physical stress.…
A clamp pressure cell for neutron scattering experiments at low temperatures and in external magnetic fields under pressure up to 2~GPa has been fabricated and tested. The cell provides optical access to the sample space that allows…
Cells within biological tissue are constantly subjected to dynamic mechanical forces. Measuring the internal stress of tissues has proven crucial for our understanding of the role of mechanical forces in fundamental biological processes…
Numerical investigation of the interaction of electromagnetic fields with eukaryotic cells requires specifically adapted computer models. Virtual microdosimetry, used to investigate exposure, requires volumetric cell models, which are…
Accurately measuring cell stiffness is challenging due to the invasiveness of traditional methods like atomic force microscopy (AFM) and optical stretching. We introduce a non-invasive off-axis system using holographic imaging and acoustic…
Voltage distribution in sub-cellular micro-domains such as neuronal synapses, small protrusions or dendritic spines regulates the opening and closing of ionic channels, energy production and thus cellular homeostasis and excitability. Yet…
Palpation of human tissue during Minimally Invasive Surgery is hampered due to restricted access. In this extended abstract, we present a variable stiffness and dynamic force range sensor that has the potential to address this challenge.…