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We demonstrate the use of Stimulated Emission Depletion (STED) spectroscopy to map the electron-optical-phonon sideband of the ground state of the radiative transition of color centers in hexagonal boron nitride emitting at 2.0-2.2 eV, with…

Mesoscale and Nanoscale Physics · Physics 2021-05-27 Ralph N. E. Malein , Prince Khatri , Andrew J. Ramsay , Isaac J. Luxmoore

With recent developments in microscopy, such as stimulated emission depletion (STED) microscopy, far-field imaging at resolutions better than the diffraction limit is now a commercially available technique. Here, we show that, in the…

Optics · Physics 2012-05-29 Chris J. Lee , Klaus J. Boller

The spatial resolution and fluorescence signal amplitude in stimulated emission depletion (STED) microscopy is limited by the photostability of available fluorophores. Here, we show that negatively-charged silicon vacancy (SiV) centers in…

Optics · Physics 2019-10-25 Yaser Silani , Forrest Hubert , Victor M. Acosta

We demonstrate stimulated emission depletion (STED) microscopy with 20 nm gold nanospheres coated by fluorescent silica. Compared with previous demonstrations of STED with a hybrid plasmonic fluorescent label, the current implementation…

Stimulated emission depletion (STED) can achieve optical super-resolution, with the optical diffraction limit broken by the suppression on the periphery of the fluorescent focal spot. Previously, it is generally experimentally accepted that…

Optics · Physics 2015-01-07 Hao Xie , Yujia Liu , Dayong Jin , Peng Xi

Stimulated Emission Depletion (STED) microscopy has emerged as a powerful technique providing visualization of biological structures at the molecular level in living samples. In this technique, the diffraction limit is broken by selectively…

Plasmonic nanoparticles influence the absorption and emission processes of nearby emitters due to local enhancements of the illuminating radiation and the photonic density of states. Here, we use the plasmon resonance of metal nanoparticles…

Stimulated emission depletion (STED) microscopy has become a powerful imaging and localized excitation method beating the diffraction barrier for improved lateral spatial resolution in cellular imaging, lithography, etc. Due to…

Recent developments in stimulated emission depletion (STED) microscopy achieved nanometer scale resolution and showed great potential in live cell imaging. Yet, STED nanoscopy techniques are based on single point-scanning. This constitutes…

Biological Physics · Physics 2015-06-16 Bin Yang , Frédéric Przybilla , Michael Mestre , Jean-Baptiste Trebbia , Brahim Lounis

We propose a novel stimulated emission depletion (STED) microscopy based on array detection and photon reassignment. By replacing the single-point detector in traditional STED with a detector array and utilizing the photon reassignment…

Photobleaching is a major limitation of superresolution Stimulated Depletion Emission (STED) microscopy. Fast scanning has long been considered an effective means to reduce photobleaching in fluorescence microscopy, but a careful…

Biological Physics · Physics 2015-10-07 Yong Wu , Xundong Wu , Rong Lu , Jin Zhang , Ligia Toro , Enrico Stefani

Magnetic particle imaging (MPI) is an in-vivo imaging method to detect magnetic nanoparticles for blood vessel imaging and molecular target imaging. Compared with conventional molecular imaging devices (such as nuclear medicine imaging PET…

Medical Physics · Physics 2024-04-26 Guang Jia , Zhongwei Bian , Tianshu Li , Shi Bai , Chenxing Hu , Lixuan Zhao , Peng Gao , Tanping Li , Hui Hui , Jie Tian

Stimulated Emission Depletion Microscopy (STED) can achieve a spatial resolution as high as several nanometers. As a point scanning imaging method, it requires 3D scanning to complete the imaging of 3D samples. The time-consuming 3D…

Optics · Physics 2024-03-06 Yihong Ji , Danni Chen , Hanzhe Wu , Gan Xiang , Heng Li , Bin Yu , Junle Qu

Accurate three-dimensional (3D) imaging requires an isotropic point spread function (PSF). However, the inherent missing aperture of a single objective lens results in an elongated, cigar-like PSF, which has rendered isotropic resolution in…

We report on multicolor excitation experiments with color centers in hexagonal boron nitride at cryogenic temperatures. We demonstrate controllable optical switching between bright and dark states of color centers emitting around 2eV.…

Mesoscale and Nanoscale Physics · Physics 2020-05-13 Prince Khatri , Andrew J. Ramsay , Ralph N. E. Malein , Harold M. H. Chong , Isaac J. Luxmoore

Two-dimensional hexagonal boron nitride offers intriguing opportunities for advanced studies of light-matter interaction at the nanoscale, specifically for realizations in quantum nanophotonics. Here, we demonstrate the engineering of…

Applied Physics · Physics 2020-04-20 Mehran Kianinia , Simon White , Johannes E. Fröch , Carlo Bradac , Igor Aharonovich

Defect centers in hexagonal boron nitride have been extensively studied as room temperature single photon sources. The electronic structure of these defects exhibits strong coupling to phonons, as evidenced by the observation of phonon…

We introduce the concept of saturated absorption competition (SAC) microscopy as a means of providing sub-diffraction spatial resolution in fluorescence imaging. Unlike the post-competition process between stimulated and spontaneous…

We demonstrate super-resolution imaging of single rare-earth emitting centers, namely, trivalent cerium, in yttrium aluminum garnet (YAG) crystals by means of stimulated emission depletion (STED) microscopy. The achieved all-optical…

Optics · Physics 2018-01-24 R. Kolesov , S. Lasse , C. Rothfuchs , A. D. Wieck , K. Xia , T. Kornher , J. Wrachtrup

Stimulated emission is the process fundamental to laser operation, thereby producing coherent photon output. Despite negatively-charged nitrogen-vacancy (NV$^-$) centres being discussed as a potential laser medium since the 1980's, there…

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