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Coherence properties and wavelength of light sources are indispensable for optical coherence microscopy/tomography as they greatly influence the signal to noise ratio, axial resolution, and penetration depth of the system. In the present…

There is widespread interest in estimating the fluorescence properties of natural materials in an image. However, the separation between reflected and fluoresced components is difficult, because it is impossible to distinguish reflected and…

Computer Vision and Pattern Recognition · Computer Science 2016-05-16 Henryk Blasinski , Joyce Farrell , Brian Wandell

We present a novel extended-focus optical coherence microscope (OCM) attaining 0.7 {\mu}m axial and 0.4 {\mu}m lateral resolution maintained over a depth of 40 {\mu}m, while preserving the advantages of Fourier domain OCM. Our method uses…

We propose a solution for increasing the axial resolution of confocal microscopes. In the experimental set-up described in this paper an interference phenomenon between two counterpropagating beams is used to determine the axial position of…

We introduce a novel laser-scanning optical microscopy technique that employs optical-frequency-comb (OFC) lasers. This method facilitates multimodal spectroscopic imaging by analyzing interferograms produced via a dual-comb spectroscopic…

We study three-dimensional microlensing where two lenses are located at different distances along the line of sight. We formulate the lens equation in complex notations and recover several previous results. There are in total either 4 or 6…

Astrophysics of Galaxies · Physics 2015-06-17 Shude Mao , Hans J. Witt , Jin H. An

A recently introduced two-channel confocal microscope with correlated detection promises up to 50% improvement in transverse spatial resolution [Simon, Sergienko, Optics Express {\bf 18}, 9765 (2010)] via the use of photon correlations.…

Quantum Physics · Physics 2015-05-18 D. S. Simon , A. V. Sergienko

In light-sheet microscopy, overall image content and resolution are improved by acquiring and fusing multiple views of the sample from different directions. State-of-the-art multi-view (MV) deconvolution employs the point spread functions…

Quantitative Methods · Quantitative Biology 2015-03-30 Benjamin Schmid , Jan Huisken

Real-time seeing and outer scale estimation at the location of the focus of a telescope is fundamental for the adaptive optics systems dimensioning and performance prediction, as well as for the operational aspects of instruments. This…

Instrumentation and Methods for Astrophysics · Physics 2015-06-22 Patrice Martinez

Fast, volumetric imaging over large scales has been a long-standing goal in biological microscopy. Scanning techniques such as fluorescence confocal microscopy can acquire 2D images at high resolution and high speed, but extending the…

Volumetric imaging by fluorescence microscopy is often limited by anisotropic spatial resolution from inferior axial resolution compared to the lateral resolution. To address this problem, here we present a deep-learning-enabled…

Computer Vision and Pattern Recognition · Computer Science 2022-07-06 Hyoungjun Park , Myeongsu Na , Bumju Kim , Soohyun Park , Ki Hean Kim , Sunghoe Chang , Jong Chul Ye

The existing metasurfaces with ultrathin volume for asymmetric transmission were often constructed by metal with low efficiency in optical frequency, and could not realize the optical asymmetric transmission and focusing simultaneously.…

Optics · Physics 2021-02-26 Jianlan Xie , Shengnan Liang , Jianjun Liu , Pinghua Tang , Shuangchun Wen

Quasar microlensing offers a unique opportunity to resolve tiny sources in distant active galactic nuclei and study compact object populations in lensing galaxies. We therefore searched for microlensing-induced variability of the…

Fourier ptychographic microscopy enables gigapixel-scale imaging, with both large field-of-view and high resolution. Using a set of low-resolution images that are recorded under varying illumination angles, the goal is to computationally…

Signal Processing · Electrical Eng. & Systems 2018-03-13 Emrah Bostan , Mahdi Soltanolkotabi , David Ren , Laura Waller

Light-sheet microscopy (LSM) is a powerful imaging technique that uses a planar illumination oriented orthogonally to the detection axis. Two-photon (2P) LSM is a variant of LSM that exploits the 2P absorption effect for sample excitation.…

Purpose: To evaluate nerve fiber layer (NFL) reflectance for glaucoma diagnosis. Methods: Participants were imaged with 4.5X4.5-mm volumetric disc scans using spectral-domain optical coherence tomography (OCT). The normalized NFL…

Image and Video Processing · Electrical Eng. & Systems 2020-06-25 Ou Tan , Liang Liu , Qisheng You , Jie Wang , Aiyin Chen , Eliesa Ing , John C. Morrison , Yali Jia , David Huang

Microscopy is an essential tool in scientific research, enabling the visualization of structures at micro- and nanoscale resolutions. However, the field of microscopy often encounters limitations in field-of-view (FOV), restricting the…

Image and Video Processing · Electrical Eng. & Systems 2025-03-17 Huanhuan Zhao , Ruben Millan-Solsona , Marti Checa , Spenser R. Brown , Jennifer L. Morrell-Falvey , Liam Collins , Arpan Biswas

We propose a method for designing multifocal diffractive lenses generating prescribed sets of foci with fixed positions at several different wavelengths. The method is based on minimizing the difference between the complex amplitudes of the…

We demonstrate sub-wavelength sectioning on biological samples with a conventional confocal microscope. This optical sectioning is achieved by the phenomenon of supercritical angle fuorescence, wherein only a fluorophore next to the…

Improving the resolution of fluorescence microscopy beyond the diffraction limit can be achievedby acquiring and processing multiple images of the sample under different illumination conditions.One of the simplest techniques, Random…