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Reconstituted nicotinic acetylcholine receptors (nAChRs) exhibit significant gain-of-function upon addition of cholesterol to reconstitution mixtures, and cholesterol affects organization of nAChRs within domain-forming membranes, but…

Subcellular Processes · Quantitative Biology 2018-12-04 Liam Sharp , Reza Salari , Grace Brannigan

At the neuromuscular junction (NMJ), the nicotinic acetylcholine receptor (nAChR) self-associates to give rise to rapid muscle movement. While lipid domains have maintained nAChR aggregates in-vitro, their specific roles in nAChR clustering…

Biological Physics · Physics 2019-07-03 Kristen Woods , Liam Sharp , Grace Brannigan

The nicotinic acetylcholine receptor (nAChR) is the prototypic member of the `Cys-loop' superfamily of ligand-gated ion channels which mediate synaptic neurotransmission, and whose other members include receptors for glycine,…

Biomolecules · Quantitative Biology 2019-07-02 Oliver Beckstein , Mark S. P. Sansom

Translational motion of neurotransmitter receptors is key for determining receptor number at the synapse and hence, synaptic efficacy. We combine live-cell STORM superresolution microscopy of nicotinic acetylcholine receptor (nAChR) with…

Biological Physics · Physics 2018-09-07 Alejo Mosqueira , Pablo A. Camino , Francisco J. Barrantes

The supramolecular assembly of lipids into bilayer membranes is essential for cellular structure and function. However, the impact of lipid structural variations such as acyl chain length, degree of unsaturation, and headgroup type on…

Soft Condensed Matter · Physics 2024-12-13 Sonam Phuntsho

To what extent must a hydrophobic gate expand for the channel to count as open? We address this question using the nicotinic acetylcholine receptor (nAChR) as the exemplar. The nAChR is an integral membrane protein which forms a cation…

Biomolecules · Quantitative Biology 2009-02-12 Sarah E. Rogers , Kaihsu Tai , Oliver Beckstein , Mark S. P. Sansom

Phospholipids are essential building blocks of biological membranes. Despite of vast amount of accurate experimental data the atomistic resolution structures sampled by the glycerol backbone and choline headgroup in phoshatidylcholine…

The cell membrane is inherently asymmetric and heterogeneous in its composition, a feature that is crucial for its function. Using atomistic molecular dynamics simulations, the physical properties of a 3-component asymmetric mixed lipid…

Soft Condensed Matter · Physics 2012-04-04 Anirban Polley , Satyavani Vemparala , Madan Rao

Monolayer spontaneous curvatures for cholesterol, DOPE, POPE, DOPC, DPPC, DSPC, POPC, SOPC, and egg sphingomyelin were obtained using small-angle X-ray scattering (SAXS) on inverted hexagonal phases (HII). Spontaneous curvatures of bilayer…

Soft Condensed Matter · Physics 2013-10-08 Benjamin Kollmitzer , Peter Heftberger , Michael Rappolt , Georg Pabst

Mechanotransduction, the biological response to mechanical stress, is often initiated by the activation of mechanosensitive (MS) proteins upon mechanically induced deformations of the cell membrane. A current challenge to fully understand…

Chemical Physics · Physics 2021-06-30 Wenjuan Jiang , Yichun Lin , Yun Lyna Luo

Although 1-alkanols are widely used as anesthetics and membrane-active agents, the molecular basis of their chain-length-dependent cutoff behavior remains unclear. Here, we perform extensive atomistic molecular dynamics simulations to…

Soft Condensed Matter · Physics 2026-01-27 Anirban Polley

We report a combined dynamic light scattering and neutron spin-echo (NSE) study on vesicles composed of the phospholipid 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine under the influence of varying amounts of perfluorooctanoic acid. We…

Soft Condensed Matter · Physics 2014-05-06 Beate-Annette Bruening , Bela Farago

The pathogenesis and progression of many tumors, including hematologic malignancies is highly dependent on enhanced lipogenesis. De novo fatty-acid synthesis permits accelerated proliferation of tumor cells by providing structural…

Tissues and Organs · Quantitative Biology 2016-12-04 Christian Thiede , Gerhard Ehninger , Kai Simons , Michal Grzybek , Adam Stefanko

We present a new approach to nicotinic receptor kinetics and a new model explaining random variabilities in the duration of open events. The model gives new interpretation on brief and long receptor openings and predicts (for two identical…

Neurons and Cognition · Quantitative Biology 2009-01-07 Ewa Nurowska , Mykola Bratiichuk , Beata Dworakowska , Roman J. Nowak

Protein fibril accumulation at interfaces is an important step in many physiological processes and neurodegenerative diseases as well as in designing materials. Here we show, using $\beta$-lactoglobulin fibrils as a model, that semiflexible…

Biomolecules · Quantitative Biology 2015-05-20 Sophia Jordens , Emily E. Riley , Ivan Usov , Lucio Isa , Peter D. Olmsted , Raffaele Mezzenga

The connection between membrane inhomogeneity and the structural basis of lipid rafts has sparked interest in the lateral organization of model lipid bilayers of two and three components. In an effort to investigate anisotropic lipid…

Mesoscale and Nanoscale Physics · Physics 2010-08-31 Paul Tumaneng , Sagar A. Pandit , Guijun Zhao , H. L. Scott

Optical nanoantennas can efficiently confine light into nanoscopic hotspots, enabling single-molecule detection sensitivity at biological relevant conditions. This innovative approach to breach the diffraction limit offers a versatile…

We consider a model of lipids in which a head group, characterized by its volume, is attached to two flexible tails of equal length. The phase diagram of the anhydrous lipid is obtained within self-consistent field theory, and displays, as…

Soft Condensed Matter · Physics 2009-10-31 Xiao-jun Li , M. Schick

Much of our understanding of the physical properties of raft domains in biological membranes, and some insight into the mechanisms underlying their formation stem from atomistic simulations of simple model systems, especially ternary…

Soft Condensed Matter · Physics 2023-03-07 Tanmoy Sarkar , Oded Farago

The anchoring of the nematic liquid crystal N-(p-methoxybenzylidene)-p-butylaniline (MBBA) on Langmuir-Blodgett monolayers of fatty acids (COOHC$_{n}$H$_{2n+1}$) was studied as a function of the length of the fatty acid alkyl chains, $n$…

Soft Condensed Matter · Physics 2009-10-31 Valentina S. U. Fazio , Francesca Nannelli , Lachezar Komitov
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