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Laser flash melting and revitrification experiments have recently improved the time resolution of cryo-electron microscopy (cryo-EM) to the microsecond timescale, making it fast enough to observe many of the protein motions that are…

Instrumentation and Detectors · Physics 2026-05-19 Wyatt A. Curtis , Constantin R. Krüger , Axel P. Tracol Gavard , Jakub Wenz , Marcel Drabbels , Ulrich J. Lorenz

Image Super-Resolution (SR) is an important class of image processing techniques to enhance the resolution of images and videos in computer vision. Recent years have witnessed remarkable progress of image super-resolution using deep…

Computer Vision and Pattern Recognition · Computer Science 2020-02-11 Zhihao Wang , Jian Chen , Steven C. H. Hoi

Cryo-electron tomography (cryoET) is a technique that captures images of biological samples at different tilts, preserving their native state as much as possible. Along with the partial tilt series and noise, one of the major challenges in…

Signal Processing · Electrical Eng. & Systems 2023-07-26 Vinith Kishore , Valentin Debarnot , Ivan Dokmanić

High-resolution structure determination by cryo-electron microscopy (cryo-EM) requires the accurate fitting of an atomic model into an experimental density map. Traditional refinement pipelines such as Phenix.real_space_refine and Rosetta…

Biomolecules · Quantitative Biology 2026-03-10 Fuyao Huang , Xiaozhu Yu , Kui Xu , Qiangfeng Cliff Zhang

Single image super-resolution (SR) aims to estimate a high-resolution (HR) image from a lowresolution (LR) input. Image priors are commonly learned to regularize the otherwise seriously ill-posed SR problem, either using external LR-HR…

Computer Vision and Pattern Recognition · Computer Science 2015-10-28 Zhangyang Wang , Yingzhen Yang , Zhaowen Wang , Shiyu Chang , Jianchao Yang , Thomas S. Huang

Background and Objective: The contrast of cryo-EM images varies from one to another, primarily due to the uneven thickness of the ice layer. This contrast variation can affect the quality of 2-D class averaging, 3-D ab-initio modeling, and…

Computer Vision and Pattern Recognition · Computer Science 2022-07-01 Yunpeng Shi , Amit Singer

Magnetic resonance imaging (MRI) revolutionized diagnostic medicine and biomedical research by allowing a noninvasive access to spin ensembles. To enhance MRI resolution to the nanometer scale, new approaches including scanning probe…

Mesoscale and Nanoscale Physics · Physics 2019-10-23 Philip Willke , Kai Yang , Yujeong Bae , Andreas J. Heinrich , Christopher P. Lutz

Deep learning techniques have been applied in the context of image super-resolution (SR), achieving remarkable advances in terms of reconstruction performance. Existing techniques typically employ highly complex model structures which…

Image and Video Processing · Electrical Eng. & Systems 2024-11-22 Yuxuan Jiang , Jakub Nawala , Fan Zhang , David Bull

Current deep image super-resolution (SR) approaches aim to restore high-resolution images from down-sampled images or by assuming degradation from simple Gaussian kernels and additive noises. However, these techniques only assume crude…

Computer Vision and Pattern Recognition · Computer Science 2024-09-06 Hu Wang , Congbo Ma , Jianpeng Zhang , Wei Emma Zhang , Gustavo Carneiro

A central problem in neuroscience is reconstructing neuronal circuits on the synapse level. Due to a wide range of scales in brain architecture such reconstruction requires imaging that is both high-resolution and high-throughput. Existing…

Computer Vision and Pattern Recognition · Computer Science 2012-10-03 Tao Hu , Juan Nunez-Iglesias , Shiv Vitaladevuni , Lou Scheffer , Shan Xu , Mehdi Bolorizadeh , Harald Hess , Richard Fetter , Dmitri Chklovskii

The scanning electron microscopy (SEM) is probably one the most fascinating examination approach that has been used since more than two decades to detailed inspection of micro scale objects. Most of the scanning electron microscopes could…

Computer Vision and Pattern Recognition · Computer Science 2016-02-18 Wichai Shanklin

Cryo Focused Ion-Beam Scanning Electron Microscopy (cryo FIB-SEM) enables three-dimensional and nanoscale imaging of biological specimens via a slice and view mechanism. The FIB-SEM experiments are, however, limited by a slow (typically,…

Most of the existing learning-based single image superresolution (SISR) methods are trained and evaluated on simulated datasets, where the low-resolution (LR) images are generated by applying a simple and uniform degradation (i.e., bicubic…

Computer Vision and Pattern Recognition · Computer Science 2019-04-02 Jianrui Cai , Hui Zeng , Hongwei Yong , Zisheng Cao , Lei Zhang

Microsecond time-resolved cryo-electron microscopy has emerged as a novel approach for directly observing proteins dynamics. By providing microsecond temporal and near-atomic spatial resolution, it has the potential to elucidate a wide…

Biological Physics · Physics 2024-02-27 Ulrich J. Lorenz

The single-particle cryo-EM field faces the persistent challenge of preferred orientation, lacking general computational solutions. We introduce cryoPROS, an AI-based approach designed to address the above issue. By generating the auxiliary…

Biomolecules · Quantitative Biology 2023-09-27 Hui Zhang , Dihan Zheng , Qiurong Wu , Nieng Yan , Zuoqiang Shi , Mingxu Hu , Chenglong Bao

Endoscopic ultrasound (EUS) imaging has a trade-off between resolution and penetration depth. By considering the in-vivo characteristics of human organs, it is necessary to provide clinicians with appropriate hardware specifications for…

Image and Video Processing · Electrical Eng. & Systems 2023-09-14 Hah Min Lew , Jae Seong Kim , Moon Hwan Lee , Jaegeun Park , Sangyeon Youn , Hee Man Kim , Jihun Kim , Jae Youn Hwang

Scanning Electron Microscopy (SEM) is critical in nanotechnology, materials science, and biological imaging due to its high spatial resolution and depth of focus. Signal-to-noise ratio (SNR) is an essential parameter in SEM because it…

Machine Learning · Computer Science 2025-10-10 K. S. Sim , I. Bukhori , D. C. Y. Ong , K. B. Gan

The state of the art in video super-resolution (SR) are techniques based on deep learning, but they perform poorly on real-world videos (see Figure 1). The reason is that training image-pairs are commonly created by downscaling a…

Computer Vision and Pattern Recognition · Computer Science 2021-05-07 Noam Elron , Alex Itskovich , Shahar S. Yuval , Noam Levy

This paper proposes a simple, accurate, and robust approach to single image nonparametric blind Super-Resolution (SR). This task is formulated as a functional to be minimized with respect to both an intermediate super-resolved image and a…

Computer Vision and Pattern Recognition · Computer Science 2015-03-17 Wen-Ze Shao , Michael Elad

Understanding the structure of a protein complex is crucial indetermining its function. However, retrieving accurate 3D structures from microscopy images is highly challenging, particularly as many imaging modalities are two-dimensional.…

Quantitative Methods · Quantitative Biology 2021-10-18 Benjamin J. Blundell , Christian Sieben , Suliana Manley , Ed Rosten , QueeLim Ch'ng , Susan Cox
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