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Flow cytometry (FCM) is the standard multi-parameter assay for measuring single cell phenotype and functionality. It is commonly used for quantifying the relative frequencies of cell subsets in blood and disaggregated tissues. A typical…

Applications · Statistics 2020-09-01 Shai Gorsky , Cliburn Chan , Li Ma

Fuzzy clustering has become a widely used data mining technique and plays an important role in grouping, traversing and selectively using data for user specified applications. The deterministic Fuzzy C-Means (FCM) algorithm may result in…

Neural and Evolutionary Computing · Computer Science 2018-10-23 Saptarshi Sengupta , Sanchita Basak , Richard Alan Peters

Flow cytometry is a technique that measures multiple fluorescence and light scatter-associated parameters from individual cells as they flow a single file through an excitation light source. These cells are labeled with antibodies to detect…

Flow cytometry mainly used for detecting the characteristics of a number of biochemical substances based on the expression of specific markers in cells. It is particularly useful for detecting membrane surface receptors, antigens, ions, or…

Machine Learning · Computer Science 2023-03-17 Yanhua Xu

Flow cytometry is a powerful quantitative assay supporting high-throughput collection of single-cell data with a high dynamic range. For flow cytometry to yield reproducible data with a quantitative relationship to the underlying biology,…

Quantitative Methods · Quantitative Biology 2023-05-16 Jacob Beal

Flow cytometry (FC) is a single-cell profiling platform for measuring the phenotypes of individual cells from millions of cells in biological samples. FC employs high-throughput technologies and generates high-dimensional data, and hence…

Quantitative Methods · Quantitative Biology 2015-01-16 Ariful Azad

In high energy physics experiments, calorimetric data reconstruction requires a suitable clustering technique in order to obtain accurate information about the shower characteristics such as position of the shower and energy deposition.…

Nuclear Experiment · Physics 2012-04-17 Radha Pyari Sandhir , Sanjib Muhuri , Tapan Nayak

Fuzzy C-Means (FCM) is a widely used clustering method. However, FCM and its many accelerated variants have low efficiency in the mid-to-late stage of the clustering process. In this stage, all samples are involved in the update of their…

Machine Learning · Computer Science 2023-02-15 Dong Li , Shuisheng Zhou , Witold Pedrycz

In this article we present a new diagnostic approach utilizing flow-cytometry to study compounds of nanoparticle samples in solution by analysis of their scattering patterns retrieved from the cytometric measurements. As a specific…

Fluorescent Timer proteins, which spontaneously change their emission spectra over time, are valuable tools for analyzing temporal changes in cellular activities at the single-cell level. Traditional analysis of Fluorescent Timer data has…

Quantitative Methods · Quantitative Biology 2024-11-15 Masahiro Ono

Flow cytometry is a valuable technique that measures the optical properties of particles at a single-cell resolution. When deployed in the ocean, flow cytometry allows oceanographers to study different types of photosynthetic microbes…

Methodology · Statistics 2026-04-02 Ethan Pawl , François Ribalet , Paul A. Parker , Sangwon Hyun

Clustering data is a popular feature in the field of unsupervised machine learning. Most algorithms aim to find the best method to extract consistent clusters of data, but very few of them intend to cluster data that share the same…

Machine Learning · Computer Science 2022-06-22 Jean-Sébastien Dessureault , Daniel Massicotte

In this paper, several two-dimensional clustering scenarios are given. In those scenarios, soft partitioning clustering algorithms (Fuzzy C-means (FCM) and Possibilistic c-means (PCM)) are applied. Afterward, VAT is used to investigate the…

Machine Learning · Computer Science 2019-05-14 Md. Abu Bakr Siddique , Rezoana Bente Arif , Mohammad Mahmudur Rahman Khan , Zahidun Ashrafi

We introduce an image cytometer (I-CYT) for the analysis of phytoplankton in fresh and marine water environments. A linear quantification of cell numbers was observed covering several orders of magnitude using cultures of Tetraselmis and…

Quantitative Methods · Quantitative Biology 2016-11-23 J. M. Pérez , M. Jofre , P. Martínez , M. A. Yáñez , V. Catalan , A. Parker , M. Veldhuis , V. Pruneri

Clustering is a central tool in biomedical research for discovering heterogeneous patient subpopulations, where group boundaries are often diffuse rather than sharply separated. Traditional methods produce hard partitions, whereas soft…

Methodology · Statistics 2026-01-07 Qiuyi Wu , Zihan Zhu , Anru R. Zhang

Explainability for Deep Learning Models is especially important for clinical applications, where decisions of automated systems have far-reaching consequences. While various post-hoc explainable methods, such as attention visualization and…

In this paper we present clustering method is very sensitive to the initial center values, requirements on the data set too high, and cannot handle noisy data the proposal method is using information entropy to initialize the cluster…

Information Retrieval · Computer Science 2011-04-12 K. Suresh

Flow cytometry measurements are widely used in diagnostics and medical decision making. Incomplete understanding of sources of measurement uncertainty can make it difficult to distinguish autofluorescence and background sources from signals…

Quantitative Methods · Quantitative Biology 2024-12-02 Prajakta Bedekar , Megan A. Catterton , Matthew DiSalvo , Gregory A. Cooksey , Anthony J. Kearsley , Paul N. Patrone

Among various soft computing approaches for time series forecasting, Fuzzy Cognitive Maps (FCM) have shown remarkable results as a tool to model and analyze the dynamics of complex systems. FCM have similarities to recurrent neural networks…

Artificial Intelligence · Computer Science 2022-01-11 Omid Orang , Petrônio Cândido de Lima e Silva , Frederico Gadelha Guimarães

Ocean microbes are critical to both ocean ecosystems and the global climate. Flow cytometry, which measures cell optical properties in fluid samples, is routinely used in oceanographic research. Despite decades of accumulated data,…

Methodology · Statistics 2025-04-17 Sangwon Hyun , Tim Coleman , Francois Ribalet , Jacob Bien
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