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Flow cytometry is a cornerstone technique in medical and biological research, providing crucial information about cell size and granularity through forward scatter (FSC) and side scatter (SSC) signals. Despite its widespread use, the…

Optics · Physics 2024-08-29 Jaepil Jo , Herve Hugonnet , Mahn Jae Lee , YongKeun Park

Clustering plays an important role in mining big data both as a modeling technique and a preprocessing step in many data mining process implementations. Fuzzy clustering provides more flexibility than non-fuzzy methods by allowing each data…

Distributed, Parallel, and Cluster Computing · Computer Science 2018-11-26 Nasser Ghadiri , Meysam Ghaffari , Mohammad Amin Nikbakht

Flow cytometry is a technology that rapidly measures antigen-based markers associated to cells in a cell population. Although analysis of flow cytometry data has traditionally considered one or two markers at a time, there has been…

Applications · Statistics 2010-03-30 Gyemin Lee , William Finn , Clayton Scott

Fuzzy Cognitive Maps (FCMs) are considered a soft computing technique combining elements of fuzzy logic and recurrent neural networks. They found multiple application in such domains as modeling of system behavior, prediction of time…

Machine Learning · Computer Science 2021-03-16 Piotr Szwed

FcsIT is a platform-independent, open-source tool for calculating the correlation and fitting fluorescence correlation spectroscopy data. The software is written in Python and uses a powerful Dear PyGUI engine for its interface. It provides…

Quantitative Methods · Quantitative Biology 2026-04-01 Tomasz Kalwarczyk

Phytoplankton are microscopic algae responsible for roughly half of the world's photosynthesis that play a critical role in global carbon cycles and oxygen production, and measuring the abundance of their subtypes across a wide range of…

Methodology · Statistics 2025-10-08 Farhad de Sousa , François Ribalet , Jacob Bien

The random motion of molecules in living cells has consistently been reported to deviate from standard Brownian motion, a behavior coined as ``anomalous diffusion''. Fluorescence Correlation Spectroscopy (FCS) is a powerful method to…

Quantitative Methods · Quantitative Biology 2024-07-18 Nathan Quiblier , Jan-Michael Rye , Pierre Leclerc , Henri Truong , Abdelkrim Hannou , Laurent Héliot , Hugues Berry

Conducting data analysis typically involves authoring code to transform, visualize, analyze, and interpret data. Large language models (LLMs) are now capable of generating such code for simple, routine analyses. LLMs promise to democratize…

Human-Computer Interaction · Computer Science 2025-04-22 Stephen N. Freund , Brooke Simon , Emery D. Berger , Eunice Jun

Cryo-electron microscopy (cryo-EM) is a powerful technique in structural biology and drug discovery, enabling the study of biomolecules at high resolution. Significant advancements by structural biologists using cryo-EM have led to the…

Biomolecules · Quantitative Biology 2024-12-05 Yi Zhou , Yilai Li , Jing Yuan , Quanquan Gu

Amperometry is a commonly used electrochemical method for studying the process of exocytosis in real-time. Given the high precision of recording that amperometry procedures offer, the volume of data generated can span over several hundreds…

Subcellular Processes · Quantitative Biology 2023-02-07 Jeyashree Krishnan , Zeyu Lian , Pieter E. Oomen , Xiulan He , Soodabeh Majdi , Andreas Schuppert , Andrew Ewing

In this paper, a fast and practical GPU-based implementation of Fuzzy C-Means(FCM) clustering algorithm for image segmentation is proposed. First, an extensive analysis is conducted to study the dependency among the image pixels in the…

Distributed, Parallel, and Cluster Computing · Computer Science 2016-03-29 Mishal Almazrooie , Mogana Vadiveloo , Rosni Abdullah

Scanning fluorescence correlation spectroscopy (SFCS) with a scan path perpendicular to the membrane plane was introduced to measure diffusion and interactions of fluorescent components in free standing biomembranes. Using a confocal laser…

Biomolecules · Quantitative Biology 2018-06-04 Paul Müller , Petra Schwille , Thomas Weidemann

Both FCM and PCM clustering methods have been widely applied to pattern recognition and data clustering. Nevertheless, FCM is sensitive to noise and PCM occasionally generates coincident clusters. PFCM is an extension of the PCM model by…

Genomics · Quantitative Biology 2021-11-25 Shahabeddin Sotudian , Mohammad Hossein Fazel Zarandi

With the rapid advances of microarray technologies, large amounts of high-dimensional gene expression data are being generated, which poses significant computational challenges. A first step towards addressing this challenge is the use of…

Computer Vision and Pattern Recognition · Computer Science 2013-02-14 P. K. Nizar Banu , H. Hannah Inbarani

Due to its specificity, fluorescence microscopy (FM) has become a quintessential imaging tool in cell biology. However, photobleaching, phototoxicity, and related artifacts continue to limit FM's utility. Recently, it has been shown that…

Background: Fluorescent Timer proteins, which display time-dependent changes in their emission spectra, are invaluable for analyzing the temporal dynamics of cellular events at the single-cell level. We previously developed the…

Quantitative Methods · Quantitative Biology 2025-07-01 Masahiro Ono

Medical image segmentation demands an efficient and robust segmentation algorithm against noise. The conventional fuzzy c-means algorithm is an efficient clustering algorithm that is used in medical image segmentation. But FCM is highly…

Computer Vision and Pattern Recognition · Computer Science 2010-04-13 S. Zulaikha Beevi , M. Mohammed Sathik , K. Senthamaraikannan

Computed tomography perfusion (CTP) and magnetic resonance perfusion (MRP) are widely used in acute ischemic stroke assessment and other cerebrovascular conditions to generate quantitative maps of cerebral hemodynamics. While commercial…

Image and Video Processing · Electrical Eng. & Systems 2026-05-19 Marijn Borghouts , Ruisheng Su

Clustering algorithms play a pivotal role in unsupervised learning by identifying and grouping similar objects based on shared characteristics. Although traditional clustering techniques, such as hard and fuzzy center-based clustering, have…

Machine Learning · Computer Science 2025-08-13 Swagato Das , Arghya Pratihar , Swagatam Das

Quantum computing presents a promising alternative for the direct simulation of quantum systems with the potential to explore chemical problems beyond the capabilities of classical methods. However, current quantum algorithms are…