Related papers: Label-free Raman spectroscopy and machine learning…
Can a single label-free image reveal whether cancer cells were exposed to chemotherapy? We present an innovative methodology on the label-free and high-resolution imaging properties of phase holotomographic microscopy coupled with neural…
Immunotherapy constitutes a paradigm shift in cancer treatment. Its FDA approval for several indications has yielded improved prognosis for cases where traditional therapy has shown limited efficiencey. However, many patients still fail to…
Imaging Raman spectroscopy can be used to identify cancerous tissue. Traditionally, a step-by-step scanning of the sample is applied to generate a Raman image, which, however, is too slow for routine examination of patients. By transferring…
In situ measurement of cellular metabolites is still a challenge in biology. Conventional methods, such as mass spectrometry or fluorescence microscopy, would either destruct the sample or introduce strong perturbations to the functions of…
Immunotherapies have been proven to have significant therapeutic efficacy in the treatment of cancer. The last decade has seen adoptive cell therapies, such as chimeric antigen receptor T-cell (CART-cell) therapy, gain FDA approval against…
Label-free imaging has gained broad interest because of its potential to omit elaborate staining procedures which is especially relevant for in vivo use. Label-free multiphoton microscopy (MPM), for instance, exploits two-photon excitation…
We demonstrate novel instrumentation for spontaneous Raman spectroscopy in biofluids, enabling development of a portable, automated, reliable diagnostics technique requiring minimal operator expertise to quantify disease markers. Label-free…
Raman spectroscopy provides spectral information related to the specific molecular structures of substances and has been well established as a powerful tool for studying biological tissues and diagnosing diseases. This article reviews…
The differentiation between pathological subtypes of non-small cell lung cancer (NSCLC) is an essential step in guiding treatment options and prognosis. However, current clinical practice relies on multi-step staining and labelling…
Immunotherapy emerges as promising approach for treating cancer. Encouraging findings have validated the efficacy of immunotherapy medications in addressing tumors, resulting in prolonged survival rates and notable reductions in toxicity…
FT-Raman spectroscopy was employed to study (in-vitro) non-altered human colorectal tissues aiming evaluate their spectral differences. The samples were collected from 39 patients, adding 144 spectra. The results enable one estabilish 3…
Time-resolved spectral techniques play an important analysis tool in many contexts, from physical chemistry to biomedicine. Customarily, the label-free detection of analytes is manually performed by experts through the aid of classic…
The emergence of label-free microscopy techniques has significantly improved our ability to precisely characterize biochemical targets, enabling non-invasive visualization of cellular organelles and tissue organization. Each label-free…
Imaging demonstrates that preclinical and human tumors are heterogeneous, i.e. a single tumor can exhibit multiple regions that behave differently during both normal development and also in response to treatment. The large variations…
FT-Raman Spectroscopy was applied to identify biochemical alterations existing between inflammatory fibrous hyperplasia (IFH) and normal tissues of buccal mucosa. One important implication of this study is related to the cancer lesion…
Confocal Raman microscopy, a highly specific and label-free technique for the microscale study of thick samples, often presents difficulties due to weak Raman signals. Inhomogeneous samples introduce wavefront aberrations that further…
Single-walled carbon nanotubes (SWNTs) with five different C13/C12 isotope compositions and well-separated Raman peaks have been synthesized and conjugated to five targeting ligands in order to impart molecular specificity. Multiplexed…
Histopathology plays a central role in diagnosis of many diseases including solid cancers. Efforts are underway to transform this subjective art form to an objective and quantitative science. Coherent Raman imaging (CRI), a label-free…
Cytological diagnosis of follicular thyroid carcinoma (FTC) is one of major challenges in the field of endocrine oncology due to absence of evident morphological indicators. Morphological abnormalities in the nucleus are typically key…
We propose a novel approach using Multiplex-Coherent Anti-Stokes Raman Scattering (M-CARS) for la-bel-free discriminations in biomedical tissues. The strategy is based on the evaluation of the contrast be-tween resonant and nonresonant…