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Photoacoustic imaging has shown great promise for medical imaging, where optical energy absorption by blood haemoglobin is used as the contrast mechanism. A numerical method was developed for the in-silico assessment of the photoacoustic…

Medical Physics · Physics 2015-12-22 Kamyar Firouzi , Nader Saffari

Many microscopy applications are limited by the total amount of usable light and are consequently challenged by the resulting levels of noise in the acquired images. This problem is often addressed via (supervised) deep learning based…

Image and Video Processing · Electrical Eng. & Systems 2020-08-20 Anna S. Goncharova , Alf Honigmann , Florian Jug , Alexander Krull

Fourier light-field microscopy (FLFM) uses a micro-lens array (MLA) to segment the Fourier Plane of the microscopic objective lens to generate multiple two-dimensional perspective views, thereby reconstructing the three-dimensional(3D)…

Optics · Physics 2022-08-29 Haixin Huang , Haoyuan Qiu , Hanzhe Wu , Yihong Ji , Heng Li , Bin Yu , Danni Chen , Junle Qu

In deep tissue photoacoustic imaging, the spatial resolution is inherently limited by acoustic diffraction. Moreover, as the ultrasound attenuation increases with frequency, resolution is often traded-off for penetration depth. Here we…

In deep tissue photoacoustic imaging the spatial resolution is inherently limited by the acoustic wavelength. We present an approach for surpassing the acoustic diffraction limit by exploiting temporal fluctuations in the sample absorption…

Optics · Physics 2017-05-31 Thomas Chaigne , Bastien Arnal , Sergey Vilov , Emmanuel Bossy , Ori Katz

Diffusion-weighted magnetic resonance imaging (DW-MRI) is a critical imaging method for capturing and modeling tissue microarchitecture at a millimeter scale. A common practice to model the measured DW-MRI signal is via fiber orientation…

Optical-resolution photoacoustic microscopy has been validated as a high-resolution and high-sensitivity imaging modality for angiographic studies in the past decades. Quantitative vascular analysis reveals critical information of…

Medical Physics · Physics 2022-06-02 Dong Zhang , Ran Li , Xin Lou , Jianwen Luo

Deconvolution microscopy has been extensively used to improve the resolution of the widefield fluorescent microscopy. Conventional approaches, which usually require the point spread function (PSF) measurement or blind estimation, are…

Machine Learning · Computer Science 2019-04-08 Sungjun Lim , Sang-Eun Lee , Sunghoe Chang , Jong Chul Ye

Photoacoustic microscopy (PAM) has been a promising biomedical imaging technology in recent years. However, the point-by-point scanning mechanism results in low-speed imaging, which limits the application of PAM. Reducing sampling density…

Image and Video Processing · Electrical Eng. & Systems 2020-06-09 Jiasheng Zhou , Da He , Xiaoyu Shang , Zhendong Guo , Sung-liang Chen , Jiajia Luo

We present a two-channel deconvolution method that decomposes images into a parametric point-source channel and a pixelized extended-source channel. Based on the central idea of the deconvolution algorithm proposed by Magain, Courbin & Sohy…

Instrumentation and Methods for Astrophysics · Physics 2016-04-27 N. Cantale , F. Courbin , M. Tewes , P. Jablonka. , G. Meylan

The purpose of this study is to enable in-vivo three-dimensional (3-D) ultrasound localization microscopy (ULM) of posterior ocular microvasculature using a 256-channel system and a 1024-element matrix array, and to overcome limitations of…

One of the fundamental limits of classical optical microscopy is the diffraction limit of optical resolution. It results from the finite bandwidth of the optical transfer function (or OTF) of an optical microscope, which restricts the…

Optics · Physics 2022-03-11 Sjoerd Stallinga , Niels Radmacher , Antoine Delon , Jörg Enderlein

Malignant brain tumors are among the deadliest neoplasms with the lowest survival rates of any cancer type. In considering surgical tumor resection, suboptimal extent of resection is linked to poor clinical outcomes and lower overall…

Image and Video Processing · Electrical Eng. & Systems 2020-09-23 Benjamin R. Ecclestone , Kevan Bell , Saad Abbasi , Deepak Dinakaran , Frank K. H. van Landeghem , John R. Mackey , Paul Fieguth , Parsin Haji Reza

We present a physics-informed deep learning framework to address common limitations in Confocal Laser Scanning Microscopy (CLSM), such as diffraction limited resolution, noise, and undersampling due to low laser power conditions. The…

Materials Science · Physics 2025-01-27 Zaheer Ahmad , Junaid Shabeer , Usman Saleem , Tahir Qadeer , Abdul Sami , Zahira El Khalidi , Saad Mehmood

The process of acquiring microscopic images in life sciences often results in image degradation and corruption, characterised by the presence of noise and blur, which poses significant challenges in accurately analysing and interpreting the…

Computer Vision and Pattern Recognition · Computer Science 2023-07-18 Tomáš Chobola , Gesine Müller , Veit Dausmann , Anton Theileis , Jan Taucher , Jan Huisken , Tingying Peng

In the imaging process of an astronomical telescope, the deconvolution of its beam or Point Spread Function (PSF) is a crucial task. However, deconvolution presents a classical and challenging inverse computation problem. In scenarios where…

Instrumentation and Methods for Astrophysics · Physics 2024-03-05 Shulei Ni , Yisheng Qiu , Yunchun Chen , Zihao Song , Hao Chen , Xuejian Jiang , Huaxi Chen

We explore the impact of different telescope apertures on the image simulation and deconvolution processes within the context of a synthetic star field. Using HCIPy and Python programming, we modelled six telescope apertures namely…

Instrumentation and Methods for Astrophysics · Physics 2024-02-09 Jyotika Roychowdhury , Kevin Derby , Daewook Kim

Optic deconvolution in light microscopy (LM) refers to recovering the object details from images, revealing the ground truth of samples. Traditional explicit methods in LM rely on the point spread function (PSF) during image acquisition.…

Quantitative Methods · Quantitative Biology 2026-03-24 Rui Li , Mikhail Kudryashev , Artur Yakimovich

In the case of ground-based telescopes equipped with adaptive optics systems, the point spread function (PSF) is only poorly known or completely unknown. Moreover, an accurate modeling of the PSF is in general not available. Therefore in…

Numerical Analysis · Mathematics 2015-06-09 M. Prato , A. La Camera , S. Bonettini , S. Rebegoldi , M. Bertero , P. Boccacci

Photoacoustic Microscopy (PAM) images integrating the advantages of optical contrast and acoustic resolution have been widely used in brain studies. However, there exists a trade-off between scanning speed and image resolution. Compared…

Image and Video Processing · Electrical Eng. & Systems 2025-03-06 Kai Pan , Linyang Li , Li Lin , Pujin Cheng , Junyan Lyu , Lei Xi , Xiaoyin Tang