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We present a semi-blind, spatially-variant deconvolution technique aimed at optical microscopy that combines a local estimation step of the point spread function (PSF) and deconvolution using a spatially variant, regularized Richardson-Lucy…

Computer Vision and Pattern Recognition · Computer Science 2019-05-21 Adrian Shajkofci , Michael Liebling

Optical microscopy is an essential tool in biology and medicine. Imaging thin, yet non-flat objects in a single shot (without relying on more sophisticated sectioning setups) remains challenging as the shallow depth of field that comes with…

Image and Video Processing · Electrical Eng. & Systems 2020-10-14 Adrian Shajkofci , Michael Liebling

Acoustic-Resolution Photoacoustic Microscopy (AR-PAM) is promising for subcutaneous vascular imaging, but its spatial resolution is constrained by the Point Spread Function (PSF). Traditional deconvolution methods like Richardson-Lucy and…

Computer Vision and Pattern Recognition · Computer Science 2024-10-29 Youshen Xiao , Sheng Liao , Xuanyang Tian , Fan Zhang , Xinlong Dong , Yunhui Jiang , Xiyu Chen , Ruixi Sun , Yuyao Zhang , Fei Gao

Ultrasound is widely used in medical diagnostics allowing for accessible and powerful imaging but suffers from resolution limitations due to diffraction and the finite aperture of the imaging system, which restricts diagnostic use. The…

Computer Vision and Pattern Recognition · Computer Science 2024-08-08 Felix Duelmer , Walter Simson , Mohammad Farid Azampour , Magdalena Wysocki , Angelos Karlas , Nassir Navab

Deconvolution is the most commonly used image processing method to remove the blur caused by the point-spread-function (PSF) in optical imaging systems. While this method has been successful in deblurring, it suffers from several…

Image and Video Processing · Electrical Eng. & Systems 2019-10-10 Huangxuan Zhao , Ziwen Ke , Ningbo Chen , Ke Li , Lidai Wang , Xiaojing Gong , Wei Zheng , Liang Song , Zhicheng Liu , Dong Liang , Chengbo Liu

Microscopy is one of the most essential imaging techniques in life sciences. High-quality images are required in order to solve (potentially life-saving) biomedical research problems. Many microscopy techniques do not achieve sufficient…

Computer Vision and Pattern Recognition · Computer Science 2018-10-24 Joris Roels , Jan Aelterman , Jonas De Vylder , Hiep Luong , Yvan Saeys , Wilfried Philips

Acoustic-resolution photoacoustic microscopy (AR-PAM) is a promising tool for microvascular imaging. In the focal region, resolution of AR-PAM is determined by the ultrasound transducer and ultimately limited by acoustic diffraction. In the…

Image and Video Processing · Electrical Eng. & Systems 2021-11-22 Fei Feng , Siqi Liang , Sung-Liang Chen

Vibro-acoustography (VA) is a medical imaging method based on the difference-frequency generation produced by the mixture of two focused ultrasound beams. VA has been applied to different problems in medical imaging such as imaging bones,…

Computer Vision and Pattern Recognition · Computer Science 2012-07-17 Talita Perciano , Matthew Urban , Nelson D. A. Mascarenhas , Mostafa Fatemi , Alejandro C. Frery , Glauber T. Silva

In multi-photon microscopy (MPM), a recent in-vivo fluorescence microscopy system, the task of image restoration can be decomposed into two interlinked inverse problems: firstly, the characterization of the Point Spread Function (PSF) and…

Image and Video Processing · Electrical Eng. & Systems 2024-11-08 Julien Ajdenbaum , Emilie Chouzenoux , Claire Lefort , Ségolène Martin , Jean-Christophe Pesquet

We study the problem of deconvolution for light-sheet microscopy, where the data is corrupted by spatially varying blur and a combination of Poisson and Gaussian noise. The spatial variation of the point spread function (PSF) of a…

Photoacoustic imaging is a new imaging technology in recent years, which combines the advantages of high resolution and rich contrast of optical imaging with the advantages of high penetration depth of acoustic imaging. Photoacoustic…

Image and Video Processing · Electrical Eng. & Systems 2020-09-30 Xianlin Song , Kanggao Tang , Jianshuang Wei , Lingfang Song

Photoacoustic imaging combines the high contrast of optical imaging with the deep penetration depth of ultrasonic imaging, showing great potential in cerebrovascular disease detection. However, the ultrasonic wave suffers strong attenuation…

Medical Physics · Physics 2025-09-08 Pengcheng Wan , Fan Zhang , Yuting Shen , Hulin Zhao , Xiran Cai , Xiaohua Feng , Fei Gao

This paper addresses the problem of high-resolution Doppler blood flow estimation from an ultrafast sequence of ultrasound images. Formulating the separation of clutter and blood components as an inverse problem has been shown in the…

Image and Video Processing · Electrical Eng. & Systems 2020-07-13 Duong-Hung Pham , Adrian Basarab , Ilyess Zemmoura , Jean-Pierre Remenieras , Denis Kouame

Spreading depolarization (SD) is a self-propagating wave of near-complete neuronal depolarization that is abundant in a wide range of neurological conditions, including stroke. SD was only recently documented in humans and is now considered…

Fluorescence microscopy plays an important role in biomedical research. The depth-variant point spread function (PSF) of a fluorescence microscope produces low-quality images especially in the out-of-focus regions of thick specimens.…

Image and Video Processing · Electrical Eng. & Systems 2019-07-09 Da He , De Cai , Jiasheng Zhou , Jiajia Luo , Sung-Liang Chen

Our long-range goal is to improve current whole-heart CT calcium score by extracting quantitative features from individual calcifications. We performed deconvolution to improve small calcifications assessment which challenge conventional CT…

We present a variational Bayesian method of joint image reconstruction and point spread function (PSF) estimation when the PSF of the imaging device is only partially known. To solve this semi-blind deconvolution problem, prior…

Data Analysis, Statistics and Probability · Physics 2013-03-18 Se Un Park , Nicolas Dobigeon , Alfred O. Hero

Transient brain-wide neuroimaging on a microscopic scale is pivotal for brain research, yet current modalities face challenges in meeting such spatiotemporal requirements. Functional ultrasound (fUS) enables transient neurovascular imaging…

Medical Physics · Physics 2025-12-09 Yang Cai , Shaoyuan Yan , Long Xu , Yanfeng Zhu , Bo Li , Kailiang Xu

Optical resolution photoacoustic microscopy (OR-PAM) leverages optical focusing and acoustic detection for microscopic optical absorption imaging. Intrinsically it owns high optical lateral resolution and poor acoustic axial resolution.…

Signal Processing · Electrical Eng. & Systems 2023-09-19 Yu-Hsiang Yu , Meng-Lin Li

Super-resolution ultrasound imaging through microbubble (MB) localisation and tracking, also known as ultrasound localisation microscopy, allows non-invasive sub-diffraction resolution imaging of microvasculature in animals and humans. The…

Image and Video Processing · Electrical Eng. & Systems 2025-05-23 Su Yan , Clotilde Vié , Marcelo Lerendegui , Herman Verinaz-Jadan , Jipeng Yan , Martina Tashkova , James Burn , Bingxue Wang , Gary Frost , Kevin G. Murphy , Meng-Xing Tang
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