English
Related papers

Related papers: Miniscope3D: optimized single-shot miniature 3D fl…

200 papers

Fluorescence microscopy is essential to study biological structures and dynamics. However, existing systems suffer from a tradeoff between field-of-view (FOV), resolution, and complexity, and thus cannot fulfill the emerging need of…

Optics · Physics 2022-09-09 Yujia Xue , Qianwan Yang , Guorong Hu , Kehan Guo , Lei Tian

A simple method of constructing the 3D surface of non-transparent micro-objects by extending the depth-of-field on the whole attainable surface is presented. The series of images of a sample are recorded by the sequential movement of the…

Instrumentation and Detectors · Physics 2009-04-15 V. V. Berejnov

Fluorescence microscopy is a quintessential tool for observing cells and understanding the underlying mechanisms of life-sustaining processes of all living organisms. The problem of extracting 3D shape of mitochondria from fluorescence…

Image and Video Processing · Electrical Eng. & Systems 2023-03-06 Abhinanda R. Punnakkal , Suyog S Jadhav , Alexander Horsch , Krishna Agarwal , Dilip K. Prasad

Three-dimensional (3D) fluorescence microscopy in general requires axial scanning to capture images of a sample at different planes. Here we demonstrate that a deep convolutional neural network can be trained to virtually refocus a 2D…

Computer Vision and Pattern Recognition · Computer Science 2019-11-05 Yichen Wu , Yair Rivenson , Hongda Wang , Yilin Luo , Eyal Ben-David , Laurent A. Bentolila , Christian Pritz , Aydogan Ozcan

Single-pixel imaging has emerged as a key technique in fluorescence microscopy, where fast acquisition and reconstruction are crucial. In this context, images are reconstructed from linearly compressed measurements. In practice, total…

Image and Video Processing · Electrical Eng. & Systems 2025-07-28 Serban C. Tudosie , Valerio Gandolfi , Shivaprasad Varakkoth , Andrea Farina , Cosimo D'Andrea , Simon Arridge

Fluorescent observation of cells generally suffers from the limited axial resolution due to the elongated point spread function of the microscope optics. Consequently, three-dimensional imaging results in axial resolution being several…

Light sheet fluorescence microscopy (LSFM) has transformed the way we visualize biological tissues in three dimensions, offering high-resolution imaging while minimizing photo-induced damage to the samples. Recent breakthroughs in…

Optics · Physics 2025-05-27 Alireza Tabatabaei Mashayekh , Jeremy Witzens

Optical frequency combs have revolutionised time and frequency metrology [1, 2]. The advent of microresonator-based frequency combs ('microcombs' [3-5]) is set to lead to the miniaturisation of devices that are ideally suited to a wide…

We present a minimally-invasive endoscope based on a multimode fiber that combines photoacoustic and fluorescence sensing. From the measurement of a transmission matrix during a prior calibration step, a focused spot is produced and…

Super-resolution microscopy has revolutionized optical fluorescence imaging by improving 3D resolution by 1-2 orders of magnitude. While different methods can successfully increase the resolution, all methods share significant differences…

Biological Physics · Physics 2015-01-26 Thomas Pengo , Nicolas Olivier , Suliana Manley

We present a novel single-shot interferometric ToF camera targeted for precise 3D measurements of dynamic objects. The camera concept is based on Synthetic Wavelength Interferometry, a technique that allows retrieval of depth maps of…

Computer Vision and Pattern Recognition · Computer Science 2022-12-05 Manuel Ballester , Heming Wang , Jiren Li , Oliver Cossairt , Florian Willomitzer

Depth imaging is vital for many emerging technologies with applications in augmented reality, robotics, gesture detection, and facial recognition. These applications, however, demand compact and low-power systems beyond the capabilities of…

Optics · Physics 2019-10-29 Shane Colburn , Arka Majumdar

The correction of the aberration of transcranial focused ultrasounds is a relevant issue for enhancing various non-invasive medical treatments. Emission through multi-element phased arrays has been the most widely accepted method to reduce…

Medical Physics · Physics 2018-05-28 Marcelino Ferri , JM Bravo , Javier Redondo , JV Sánchez-Pérez

Additive manufacturing using light is commonly constrained by serial voxel-by-voxel or layer-by-layer processing, which fundamentally limits fabrication speed and scalability. Here, we introduce a single-exposure holographic…

Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…

Optics · Physics 2022-01-26 Yi Xue , David Ren , Laura Waller

High frame rate 3-D ultrasound imaging technology combined with super-resolution processing method can visualize 3-D microvascular structures by overcoming the diffraction limited resolution in every spatial direction. However, 3-D…

The present study presents a simple but effective method for 3D visualization of the two-phase flow in the micro-tube. An isosceles right-angle prism combined with a mirror located 45^o bevel to the prism is employed to obtain synchronously…

Fluid Dynamics · Physics 2009-07-12 X. Fu , P. Zhang , H. Hu , C. J. Huang , Y. Huang , R. Z. Wang

Large-scale microfluidic microsystems with complex three-dimensional (3D) configurations are highly in demand by both fundamental research and industrial application, holding the potentials for fostering a wide range of innovative…

Applied Physics · Physics 2020-01-13 Zijie Lin , Jian Xu , Yunpeng Song , Xiaolong Li , Peng Wang , Wei Chu , Zhenhua Wang , Ya Cheng

A flexible multimode fiber is an exceptionally efficient tool for in vivo deep tissue imaging. Recent advances in compressive multimode fiber sensing allow for imaging with sub-diffraction spatial resolution and sub-Nyquist speed. At…

Optics · Physics 2024-07-17 Zhouping Lyu , Shih-Te Hung , Carlas S. Smith , Lyubov V. Amitonova

Two-Photon Laser-Scanning Microscopy is a powerful tool for exploring biological structure and function because of its ability to optically section through a sample with a tight focus. While it is possible to obtain 3D image stacks by…

Biological Physics · Physics 2020-01-08 Courtney Johnson , Jack Exell , Jonathon Kuo , Kevin Welsher