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Single Molecule Localization Microscopy (SMLM) enables the acquisition of high-resolution images by alternating between activation of a sparse subset of fluorescent molecules present in a sample and localization. In this work, the…

Image and Video Processing · Electrical Eng. & Systems 2018-12-17 Arne Bechensteen , Laure Blanc-Féraud , Gilles Aubert

Performing tasks in agriculture, such as fruit monitoring or harvesting, requires perceiving the objects' spatial position. RGB-D cameras are limited under open-field environments due to lightning interferences. So, in this study, we state…

Super-resolution imaging using sub-diffraction field localization by micron sized transparent beads (microspheres) was recently demonstrated [1]. Practical applications in microscopy require control over the positioning of the microspheres.…

Optics · Physics 2013-12-25 Leonid A. Krivitsky , Jia Jun Wang , Zengbo Wang , Boris Lukiyanchuk

Accurate source localization in Multi-Platform Radar Networks (MPRNs) benefits from exploiting both range and angle measurements under robust estimation. In this paper, we propose a robust Euclidean distance matrix (EDM) optimization model…

Signal Processing · Electrical Eng. & Systems 2026-04-20 Mingyu Zhao , Qingna Li , Hou-Duo Qi

Digital fringe projection (DFP) enables micrometer-level 3D reconstruction, yet extending it to large-scale mapping remains challenging because six-degree-of-freedom pose estimation often cannot match the reconstruction's precision.…

Computer Vision and Pattern Recognition · Computer Science 2026-03-13 Sehoon Tak , Keunhee Cho , Sangpil Kim , Jae-Sang Hyun

Highly-multiplexed, robotic, fiber-fed spectroscopic surveys are observing tens of millions of stars and galaxies. For many systems, accurate positioning relies on imaging the fibers in the focal plane and feeding that information back to…

Optical near fields are at the heart of various applications in sensing and imaging. We investigate dipole scattering as a parameter estimation problem and show that optical near-fields carry more information about the location and the…

Quantum Physics · Physics 2024-06-18 Lukas Kienesberger , Thomas Juffmann , Stefan Nimmrichter

We present a novel method for precise 3D object localization in single images from a single calibrated camera using only 2D labels. No expensive 3D labels are needed. Thus, instead of using 3D labels, our model is trained with…

Computer Vision and Pattern Recognition · Computer Science 2023-11-30 Daniel Kienzle , Julian Lorenz , Katja Ludwig , Rainer Lienhart

Transformer-based methods have swept the benchmarks on 2D and 3D detection on images. Because tokenization before the attention mechanism drops the spatial information, positional encoding becomes critical for those methods. Recent works…

Computer Vision and Pattern Recognition · Computer Science 2023-07-31 Changyong Shu , JIajun Deng , Fisher Yu , Yifan Liu

Self-calibrating and dual-slope measurements have been used in the field of diffuse optics for robust assessment of absolute values or temporal changes in the optical properties of highly scattering media and biological tissue. These…

Optics · Physics 2025-05-05 Giles Blaney , Angelo Sassaroli , Tapan Das , Sergio Fantini

Recent theoretical and experimental work has shown that the quantum Fisher information associated with estimating the separation between two optical point sources remains finite at small separations, effectively opening new routes to…

A theory is formulated for time dependent fluctuations of the spectrum of a single molecule in a dynamic environment. In particular, we investigate the photon counting statistics of a single molecule undergoing a spectral diffusion process.…

Statistical Mechanics · Physics 2007-05-23 YounJoon Jung , Eli Barkai , Robert J. Silbey

The resolution of photoacoustic imaging deep inside scattering media is limited by the acoustic diffraction limit. In this work, taking inspiration from super-resolution imaging techniques developed to beat the optical diffraction limit, we…

Optics · Physics 2017-11-15 Sergey Vilov , Bastien Arnal , Emmanuel Bossy

Conventional 2D fluorescence imaging in glioma surgery cannot separate intrinsic fluorophore strength from attenuation with depth, creating depth-intensity ambiguity that can compromise assessment of residual tumour and fluorescence based…

Medical Physics · Physics 2026-04-07 Yucheng Bian , Sebastien Ourselin , Yijing Xie

We show that structural information can be extracted from single molecule localization microscopy (SMLM) data. More precisely, we reinterpret SMLM data as the measures of a phaseless optical diffraction tomography system for which the…

Signal Processing · Electrical Eng. & Systems 2023-07-19 Thanh-An Pham , Emmanuel Soubies , Ferréol Soulez , Michael Unser

Single particle reconstruction has recently emerged in 3D fluorescence microscopy as a powerful technique to improve the axial resolution and the degree of fluorescent labeling. It is based on the reconstruction of an average volume of a…

Image and Video Processing · Electrical Eng. & Systems 2023-01-24 Thibaut Eloy , Etienne Baudrier , Marine Laporte , Virginie Hamel , Paul Guichard , Denis Fortun

As a method to extract information from optical system, imaging can be viewed as a parameter estimation problem. The fundamental precision in locating one emitter or estimating the separation between two incoherent emitters is bounded below…

Quantum Physics · Physics 2021-07-29 Ben Wang , Liang Xu , Lijian Zhang

The dielectric microsphere can be directly embedded into the traditional microscope, which can significantly improve the resolution of the microscope and provide a simple and feasible way to break through the diffraction limit of optical…

Optics · Physics 2019-09-23 Guorong Sui , Fan Liu , Yuehua Zhang , Xiliang Yang , Xiangmei Dong

Estimation of an optical beam's transverse displacement is a canonical imaging problem fundamental to numerous optical imaging and sensing tasks. Quantum enhancements to the measurement precision in this problem have been studied…

Quantum Physics · Physics 2025-09-16 Wenhua He , Christos N. Gagatsos , Dalziel J. Wilson , Saikat Guha

We report a photoluminescence imaging system for locating single quantum emitters with respect to alignment features. Samples are interrogated in a 4~K closed-cycle cryostat by a high numerical aperture (NA=0.9, 100$\times$ magnification)…

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