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Imaging mass spectrometry (IMS) is a powerful tool for untargeted, highly multiplexed molecular mapping of tissue in biomedical research. IMS offers a means of mapping the spatial distributions of molecular species in biological tissue with…

Computer Vision and Pattern Recognition · Computer Science 2025-08-05 Yijie Zhang , Luzhe Huang , Nir Pillar , Yuzhu Li , Lukasz G. Migas , Raf Van de Plas , Jeffrey M. Spraggins , Aydogan Ozcan

An improved method of remote optical absorption spectroscopy and hyperspectral optical absorption imaging is described which takes advantage of the photoacoustic remote sensing detection architecture. A wide range of photoacoustic…

Biological Physics · Physics 2021-08-04 Kevan Bell , Lyazzat Mukhangaliyeva , Layla Khalili , Parsin Haji Reza

Assessment of heart sounds is the cornerstone of cardiac examination, but it requires a stethoscope, skills and experience, and a direct contact with the patient. We developed a contactless, machine-learning assisted method for heart-sound…

Digital holographic microscopy is a thriving imaging modality that attracted considerable research interest in quantitative biological cell imaging due to its ability to not only create excellent label-free contrast, but also supply…

Optics · Physics 2019-04-03 Gili Dardikman , Natan T. Shaked

Resistive Pulse Sensing (RPS) is a key label-free technology to measure particles and single-cell size distribution. As a growing corpus of evidence supports that cancer cells exhibit distinct mechanical phenotypes from healthy cells,…

Biological Physics · Physics 2020-04-01 Antoine Riaud , Anh L. P. Thai , Wei Wang , Valerie Taly

Vibrational microscopy provides label-free, bond-selective chemical contrast by detecting molecular vibrations, making it invaluable for biomedical research. While conventional methods rely on the direct detection of Raman scattering or…

Optics · Physics 2025-02-10 Keiichiro Toda , Takuro Ideguchi

Histological staining is a vital step used to diagnose various diseases and has been used for more than a century to provide contrast to tissue sections, rendering the tissue constituents visible for microscopic analysis by medical experts.…

Image and Video Processing · Electrical Eng. & Systems 2020-05-08 Yijie Zhang , Kevin de Haan , Yair Rivenson , Jingxi Li , Apostolos Delis , Aydogan Ozcan

Reflectance Confocal Microscopy (RCM) is a non-invasive imaging technique used in biomedical research and clinical dermatology. It provides virtual high-resolution images of the skin and superficial tissues, reducing the need for physical…

Image and Video Processing · Electrical Eng. & Systems 2024-04-26 Hong-Jun Yoon , Chris Keum , Alexander Witkowski , Joanna Ludzik , Tracy Petrie , Heidi A. Hanson , Sancy A. Leachman

Ultrathin lensless fibre endoscopes offer minimally invasive investigation, but they mostly operate as a rigid type due to the need for prior calibration of a fibre probe. Furthermore, most implementations work in fluorescence mode rather…

Optics · Physics 2020-10-23 Wonjun Choi , Munkyu Kang , Jin Hee Hong , Ori Katz , Youngwoon Choi , Wonshik Choi

The mesoscale characterization of biological specimens has traditionally required compromises between resolution, field-of-view, depth-of-field, and molecular specificity, with most approaches relying on external labels. Here we present the…

Objective: Non-contact physiological measurement is a growing research area that allows capturing vital signs such as heart rate (HR) and breathing rate (BR) comfortably and unobtrusively with remote devices. However, most of the approaches…

Computer Vision and Pattern Recognition · Computer Science 2018-11-21 Weixuan Chen , Javier Hernandez , Rosalind W. Picard

Coherent anti-Stokes Raman scattering (CARS) microscopy offers label-free chemical imaging capabilities, but its performance is constrained by small Raman scattering cross-section, strong non-resonant background (NRB), and limited…

This work presents a high-resolution X-ray microtomography system that uses commercial off-the-shelf (COTS) CMOS image sensors as direct detectors, relying on the sensor s intrinsic resolution to achieve tomographic reconstructions without…

Image and Video Processing · Electrical Eng. & Systems 2026-05-29 Damian L. Corzi , Jose Lipovetzky , Fabricio Alcalde Bessia , German Mato , Andres Cicuttin , Maria L. Crespo , Martin Perez , Mariano Gomez Berisso

Non-invasive morphological assessment is the cornerstone of oocyte and embryo selection in assisted reproductive technology, yet clinical practice remains limited by two-dimensional, qualitative microscopy. While three-dimensional (3D)…

Histological analysis of tissue samples is one of the most widely used methods for disease diagnosis. After taking a sample from a patient, it goes through a lengthy and laborious preparation, which stains the tissue to visualize different…

Computer Vision and Pattern Recognition · Computer Science 2019-03-05 Yair Rivenson , Hongda Wang , Zhensong Wei , Yibo Zhang , Harun Gunaydin , Aydogan Ozcan

The ability to reliably estimate physiological signals from video is a powerful tool in low-cost, pre-clinical health monitoring. In this work we propose a new approach to remote photoplethysmography (rPPG) - the measurement of blood volume…

Computer Vision and Pattern Recognition · Computer Science 2021-11-19 John Gideon , Simon Stent

This work presents a novel self-supervised pre-training method to learn efficient representations without labels on histopathology medical images utilizing magnification factors. Other state-of-theart works mainly focus on fully supervised…

Image and Video Processing · Electrical Eng. & Systems 2022-09-09 Prakash Chandra Chhipa , Richa Upadhyay , Gustav Grund Pihlgren , Rajkumar Saini , Seiichi Uchida , Marcus Liwicki

We have developed a multimodal photoacoustic remote sensing (PARS) microscope combined with swept source optical coherence tomography for in vivo, non-contact retinal imaging. Building on the proven strength of multiwavelength PARS imaging,…

Polarimetric imaging provides valuable insights into the polarization state of light interacting with a sample. It can infer crucial birefringence properties of bio-specimens without using any labels, thereby facilitating the diagnosis of…

Many essential cellular functions depend on the viscoelastic properties of the cytoplasm. While techniques such as optical tweezers and atomic force microscopy can measure these properties, their reliance on localized probes prevents…