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Super-resolution structured illumination microscopy (SR-SIM) is a widely used technique for enhancing the resolution of fluorescence imaging beyond the diffraction limit. Most existing SR-SIM methods rely on Moir\'e effect-based physical…

Optics · Physics 2025-02-27 Junzheng Peng , Jiahao Xian , Xi Lin , Manhong Yao , Shiping Li , Jingang Zhong

Super-resolution microscopy has revolutionized optical fluorescence imaging by improving 3D resolution by 1-2 orders of magnitude. While different methods can successfully increase the resolution, all methods share significant differences…

Biological Physics · Physics 2015-01-26 Thomas Pengo , Nicolas Olivier , Suliana Manley

The spatial resolution of images of living samples obtained by fluorescence microscopes is physically limited due to the diffraction of visible light, which makes the study of entities of size less than the diffraction barrier (around 200…

Image and Video Processing · Electrical Eng. & Systems 2023-03-21 Vasiliki Stergiopoulou , Subhadip Mukherjee , Luca Calatroni , Laure Blanc-Féraud

Functional Spectral Imaging (FSI) models image formation as the recovery of tissue surrogates such as density and stiffness from spectral perturbations of a self-adjoint elliptic operator. Rather than relying on reflectivity or relaxation…

Medical Physics · Physics 2025-10-21 Cesar Mello Fernando Medina da Cunha

Among super-resolution microscopy techniques, structured illumination microscopy (SIM) shows great advances of low phototoxicity, high speed, and excellent performance in long-term dynamic observation, making it especially suitable for live…

Optics · Physics 2025-02-07 Han Wang , Wenshu Wang , Xinzhu Xu , Meiqi Li , Peng Xi

Magnetic resonance imaging (MRI) plays a vital role in clinical diagnostics, yet it remains hindered by long acquisition times and motion artifacts. Multi-contrast MRI reconstruction has emerged as a promising direction by leveraging…

Computer Vision and Pattern Recognition · Computer Science 2026-01-15 Xinming Fang , Chaoyan Huang , Juncheng Li , Jun Wang , Jun Shi , Guixu Zhang

Extending super-resolution imaging techniques to objects hidden in strongly scattering media potentially revolutionize the technical analysis for much broader categories of samples, such as biological tissues. The main challenge is the…

We introduce a superresolution technique that combines spatial mode demultiplexing (SPADE) with emitter blinking. We show that temporal fluctuations not only enhance the precision of SPADE imaging, but also drastically simplify the…

Optics · Physics 2025-11-27 Stanislaw Kurdzialek

Fluorescence lifetime imaging microscopy (FLIM) is an important technique to understand the chemical micro-environment in cells and tissues since it provides additional contrast compared to conventional fluorescence imaging. When two…

Image and Video Processing · Electrical Eng. & Systems 2022-01-19 Varun Mannam , Xiaotong Yuan , Scott Howard

Perhaps surprisingly, the total electron microscopy (EM) data collected to date is less than a cubic millimeter. Consequently, there is an enormous demand in the materials and biological sciences to image at greater speed and lower dosage,…

Computer Vision and Pattern Recognition · Computer Science 2016-12-06 Suhas Sreehari , S. V. Venkatakrishnan , Katherine L. Bouman , Jeffrey P. Simmons , Lawrence F. Drummy , Charles A. Bouman

Far-field optical microscopy using focused light is an important tool in a number of scientific disciplines including chemical, (bio)physical and biomedical research, particularly with respect to the study of living cells and organisms.…

We report, for the first time, a multi-confocal Fluorescence Correlation Spectroscopy (mFCS) technique which allows parallel measurements at different locations, by combining a Spatial Light Modulator (SLM), with an Electron Multiplying-CCD…

Fourier Transform Interferometry (FTI) is an appealing Hyperspectral (HS) imaging modality for many applications demanding high spectral resolution, e.g., in fluorescence microscopy. However, the effective resolution of FTI is limited by…

Information Theory · Computer Science 2019-01-31 A. Moshtaghpour , L. Jacques , V. Cambareri , P. Antoine , M. Roblin

In the process of performing image super-resolution processing, the processing of complex localized information can have a significant impact on the quality of the image generated. Fractal features can capture the rich details of both micro…

Computer Vision and Pattern Recognition · Computer Science 2025-02-28 Lianping Yang , Peng Jiao , Jinshan Pan , Hegui Zhu , Su Guo

Fourier Ptychographic Microscopy (FPM) is a computational imaging method that is able to super-resolve features beyond the diffraction-limit set by the objective lens of a traditional microscope. This is accomplished by using synthetic…

Signal Processing · Electrical Eng. & Systems 2019-04-09 Michael Kellman , Emrah Bostan , Michael Chen , Laura Waller

We report an experimental demonstration of a nonclassical imaging mechanism with super-resolving power beyond the Rayleigh limit. When the classical image is completely blurred out due to the use of a small imaging lens, by taking advantage…

Optics · Physics 2016-11-03 Jane Sprigg , Tao Peng , Yanhua Shih

We numerically investigate the role of quantum fluctuations in superresolution of optical objects. First, we confirm that when quantum fluctuations are not taken into account, one can easily improve the resolution by one order of magnitude…

Quantum Physics · Physics 2007-05-23 Vladislav N. Beskrovnyy , Mikhail I. Kolobov

Diffusion-based image super-resolution (SR) has recently attracted significant attention by leveraging the expressive power of large pre-trained text-to-image diffusion models (DMs). A central practical challenge is resolving the trade-off…

Image and Video Processing · Electrical Eng. & Systems 2026-01-26 Maxence Noble , Gonzalo Iñaki Quintana , Benjamin Aubin , Clément Chadebec

Fluorescence microscopy is rapidly turning into nanoscopy. Among the various nanoscopy methods, the STED/RESOLFT super-resolution family has recently been expanded to image even large fields of view within a few seconds. This advance relies…

Correlative microscopy is a powerful technique that combines the advantages of multiple imaging modalities to achieve a comprehensive understanding of investigated samples. For example, fluorescence microscopy provides unique functional…