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Wide-field fluorescence microscopy, while much faster than confocal microscopy, suffers from a lack of optical sectioning and poor axial resolution. 3D structured illumination microscopy (SIM) has been demonstrated to provide optical…

By exploiting the nonlinear responses of the fluorescent probes, the spatial resolution of structured illumination microscopy(SIM) can be further increased. However, due to the complex reconstruction process, the traditional reconstruction…

Structured illumination microscopy (SIM) achieves superresolution in fluorescence imaging through patterned illumination and computational image reconstruction, yet current methods require bulky, costly modulation optics and high-precision…

Optics · Physics 2024-04-15 Tamal Roy , Peter T. Brown , Douglas P. Shepherd , Lisa V. Poulikakos

Recently, chiral structured illumination microscopy has been proposed to image fluorescent chiral domains at sub-wavelength resolution. Chiral structured illumination microscopy is based on the combination of structured illumination…

Optics · Physics 2021-07-28 Shiang-Yu Huang , Ankit Kumar Singh , Jer-Shing Huang

In this communication, a fast reconstruction algorithm is proposed for fluorescence \textit{blind} structured illumination microscopy (SIM) under the sample positivity constraint. This new algorithm is by far simpler and faster than…

Data Analysis, Statistics and Probability · Physics 2016-11-18 S. Labouesse , M. Allain , J. Idier , S. Bourguignon , A. Negash , P. Liu , A. Sentenac

Structured illumination microscopy (SIM) is a pivotal technique for dynamic subcellular imaging in live cells. Conventional SIM reconstruction algorithms depend on accurately estimating the illumination pattern and can introduce artefacts…

Structured illumination microscopy (SIM) is a wide-field super-resolution technique normally limited to roughly twice the diffraction-limited resolution ($\approx 100$--$200$~nm). Surpassing this bound is a classic ill-posed inverse…

The blind structured illumination microscopy (SIM) strategy proposed in (Mudry et al., 1992) is fully re-founded in this paper, unveiling the central role of the sparsity of the illumination patterns in the mechanism that drives…

Data Analysis, Statistics and Probability · Physics 2017-04-26 Simon Labouesse , Awoke Negash , Jérôme Idier , Sébastien Bourguignon , Thomas Mangeat , Penghuan Liu , Anne Sentenac , Marc Allain

We propose to enhance the performance of localized plasmon structured illumination microscopy (LP-SIM) via intensity correlations. LP-SIM uses sub-wavelength illumination patterns to encode high spatial frequency information. It can enhance…

Structured illumination enables the tailoring of an imaging device's optical transfer function to enhance resolution. We propose the incorporation of a temporal periodic modulation, specifically a rotating mask, to encode multiple transfer…

Optics · Physics 2023-06-27 Guillaume Noetinger , Fabrice Lemoult , Sébastien M. Popoff

The rapid development of facial manipulation techniques has aroused public concerns in recent years. Following the success of deep learning, existing methods always formulate DeepFake video detection as a binary classification problem and…

Computer Vision and Pattern Recognition · Computer Science 2021-10-12 Zhihao Gu , Yang Chen , Taiping Yao , Shouhong Ding , Jilin Li , Feiyue Huang , Lizhuang Ma

Structured illumination microscopy (SIM) achieves doubled spatial resolution by exciting the specimen with a high-contrast, high-frequency sinusoidal pattern. Such an excitation pattern can be generated by interference between multiple…

Optics · Physics 2020-07-15 Meiqi Li , Yaning Li , Wenhui Liu , Karl Zhanghao , Peng Xi

High-content biological microscopy targets high-resolution imaging across large fields-of-view (FOVs). Recent works have demonstrated that computational imaging can provide efficient solutions for high-content microscopy. Here, we use…

Image and Video Processing · Electrical Eng. & Systems 2019-03-28 Li-Hao Yeh , Shwetadwip Chowdhury , Laura Waller

A novel approach to improving the performances of confocal scanning imaging is proposed. We experimentally demonstrate its feasibility using acoustic waves. It relies on a new way to encode spatial information using the temporal dimension.…

The Structural Similarity (SSIM) Index is a very widely used image/video quality model that continues to play an important role in the perceptual evaluation of compression algorithms, encoding recipes and numerous other image/video…

Image and Video Processing · Electrical Eng. & Systems 2021-02-12 Abhinau K. Venkataramanan , Chengyang Wu , Alan C. Bovik , Ioannis Katsavounidis , Zafar Shahid

The partially separable functions (PSF) model is commonly adopted in dynamic MRI reconstruction, as is the underlying signal model in many reconstruction methods including the ones relying on low-rank assumptions. Even though the PSF model…

Image and Video Processing · Electrical Eng. & Systems 2026-01-21 Rodrigo A. Lobos , Xiaokai Wang , Rex T. L. Fung , Yongli He , David Frey , Dinank Gupta , Zhongming Liu , Jeffrey A. Fessler , Douglas C. Noll

Sub-diffraction resolution, gentle sample illumination, and the possibility to image in multiple colors make Structured Illumination Microscopy (SIM) an imaging technique which is particularly well suited for live cell observations. Here,…

Structured illumination microscopy (SIM) has emerged as a widely adopted super-resolution fluorescence imaging modality, offering high speed, low phototoxicity, large field-of-view, and compatibility with conventional probes. However, when…

Image and Video Processing · Electrical Eng. & Systems 2026-03-19 Jiaming Qian , Jing Feng , Hongjun Wu , Maoxian Zhang , Dongqin Lu , Tianchi Kang , Xinyu Han , Qian Chen , Chao Zuo

We present experimental demonstration of tilt-mirror assisted transmission structured illumination microscopy (tSIM) that offers a large field of view super resolution imaging. An assembly of custom-designed tilt-mirrors are employed as the…

Modern optical microscopy methods have been advanced to provide super resolution at high imaging speed, but not chirality discriminative. We recently proposed chiral structured-illumination microscopy (SIM) method to image chiral…

Optics · Physics 2021-03-11 Jiwei Zhang , Shiang-Yu Huang , Ankit Kumar Singh , Jer-Shing Huang