Related papers: The point spread function in interferometric scatt…
Interferometric scattering microscopy (iSCAT) can image the dynamics of nanometer-scale systems. The typical approach to analyzing interferometric images involves intensive processing, which discards data and limits the precision of…
Interferometric scattering (iSCAT) microscopy is currently among the most powerful techniques available for achieving high-sensitivity single-particle localization. This capability is realized through homodyne detection, where interference…
Interferometric scattering (iSCAT) microscopy is a powerful tool for label-free sensitive detection and imaging of nanoparticles to high spatio-temporal resolution. As it was born out of detection principles central to conventional…
Interferometric scattering microscopy (iSCAT), as an ultrasensitive fluorescence-free imaging modality, has recently gain enormous attention and been rapidly developing from demonstration of principle to quantitative sensing. Here we report…
Tracking nanoparticle movement is highly desirable in many scientific areas, and various imaging methods have been employed to achieve this goal. Interferometric scattering (iSCAT) microscopy has been particularly successful in combining…
Interferometric scattering (iSCAT) microscopy has undergone significant development in recent years. It is a promising technique for imaging and tracking nanoscopic label-free objects with nanometer localization precision. The current…
The point spread function (PSF) is fundamental to any type of microscopy, most importantly so for single-molecule localization techniques, where the exact PSF shape is crucial for precise molecule localization at the nanoscale. However,…
We provide detailed experimental guidelines for implementing digital holography in the context of high-sensitivity interferometric scattering (iSCAT) based nanosizing applications. Our approach relies on interferometry via the highly…
Direct optical detection and imaging of single nanoparticles on substrate in wide field underpin vast applications across different research fields. However, the speckles originating from the unavoidable random surface undulations of the…
Coherent interferometric scattering microscopy (iscat) enables nanoparticle tracking on microsecond timescales and with nanometer precision, and has become a key tool in structural and cellular biophysics. The achievable localization…
The ability to observe rotational dynamics and measure underlying torques is a crucial component in understanding the function and mechanics of nanoscale systems. Yet, direct observation of rotational dynamics at the single-molecule level…
Nanoscale localization of point emitters is critical to several methods in optical fluorescence microscopy, including single-molecule super-resolution imaging and tracking. While the precision of the localization procedure has been the…
Nanoparticles (NPs) have proven their applicability in biosensing, drug delivery, and photo-thermal therapy, but their performance depends critically on the distribution and number of functional groups on their surface. When studying…
We experimentally demonstrate a non-imaging approach to displacement measurement for complex scattering materials. By spatially controlling the wave front of the light that incidents on the material we concentrate the scattered light in a…
This Point spread function (PSF) plays a crucial role in many computational imaging applications, such as shape from focus/defocus, depth estimation, and fluorescence microscopy. However, the mathematical model of the defocus process is…
The ability to spatially and temporally map nanoscale environments in situ over extended timescales would be transformative for biology, biomedicine, and bioengineering. All nanometer objects, from nanoparticles down to single proteins,…
Interferometric scattering microscopy is a powerful technique that enables various applications, such as mass photometry and particle tracking. Here we present a numerical toolbox to simulate images obtained in interferometric scattering,…
Localization microscopy is an imaging technique in which the positions of individual nanoscale point emitters (e.g. fluorescent molecules) are determined at high precision from their images. This is the key ingredient in…
The key features of the MATPHOT algorithm for precise and accurate stellar photometry and astrometry using discrete Point Spread Functions are described. A discrete Point Spread Function (PSF) is a sampled version of a continuous PSF which…
Interferometric imaging is an emerging technique for particle tracking and mass photometry. Mass or position are estimated from weak signals, coherently scattered from nanoparticles or single molecules, and interfered with a co-propagating…