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Ptychography is a computational imaging technique that has risen in popularity in the x-ray and electron microscopy communities in the past half decade. One of the reasons for this success is the development of new high performance electron…

Computational Physics · Physics 2023-09-26 Anton Gladyshev , Thomas C. Pekin , Marcel Schloz , Benedikt Haas , Johannes Müller , Christoph T. Koch

Molecular and atomic imaging required the development of electron and scanning probe microscopies to surpass the physical limits dictated by diffraction. Nano-infrared experiments and pico-cavity tip-enhanced Raman spectroscopy imaging…

Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution…

Biological Physics · Physics 2007-08-27 James H. Rice

The relative roles of multiple electron scattering and in-molecule free-space propagation in transmission electron microscopy of small molecules are discussed. It is argued that while multiple scattering tends to have only a moderate effect…

Image and Video Processing · Electrical Eng. & Systems 2019-12-02 T. E. Gureyev , H. M. Quiney , A. Kozlov , L. J. Allen

Using a high energy electron beam for the imaging of high density matter with both high spatial-temporal and areal density resolution under extreme states of temperature and pressure is one of the critical challenges in high energy density…

We derive upper bounds to free-space concentration of electromagnetic waves, mapping out the limits to maximum intensity for any spot size and optical beam-shaping device. For sub-diffraction-limited optical beams, our bounds suggest the…

Optics · Physics 2020-07-08 Hyungki Shim , Haejun Chung , Owen D. Miller

We report a new method for quantitative estimation of graphene layer thicknesses using high contrast imaging of graphene films on insulating substrates with a scanning electron microscope. By detecting the attenuation of secondary electrons…

Mesoscale and Nanoscale Physics · Physics 2012-03-28 Vidya Kochat , Atindra Nath Pal , Sneha E. S. , Arjun B. S. , Anshita Gairola , S. A. Shivashankar , Srinivasan Raghavan , Arindam Ghosh

Existing methods in nanoscale connectomics are at present too slow to map entire mammalian brains. As an emerging approach, expansion microscopy (ExM) has enormous promise, yet it still suffers from throughput limitations. Mapping the human…

Quantitative Methods · Quantitative Biology 2026-01-21 Logan Thrasher Collins

A notorious problem in high-resolution biological electron microscopy is radiation damage to the specimen caused by probe electrons. Hence, acquisition of data with minimal number of electrons is of critical importance. Quantum approaches…

Quantum Physics · Physics 2014-03-05 Hiroshi Okamoto , Yukinori Nagatani

We demonstrate that powder diffraction data can be collected from sub-micron crystals of a mbrane protein with nearly two orders of magnitude more atoms than the molecules commonly used for powder diffraction. The crystals of photosystem-1…

In cultural heritage conservation, it is increasingly common to rely on non-destructive imaging methods based on the absorption or scattering of photons ($X$ or $\gamma$ rays) or neutrons. However, physical and practical issues limit these…

Coherent X-ray diffraction microscopy is a method of imaging non-periodic isolated objects at resolutions only limited, in principle, by the largest scattering angles recorded. We demonstrate X-ray diffraction imaging with high resolution…

Hard x-ray imaging is indispensable across diverse fields owing to its high penetrability. However, the resolution of traditional x-ray imaging modalities, such as computed tomography (CT) systems, is constrained by factors including beam…

Medical Physics · Physics 2024-02-23 O. Sefi , A. Ben Yehuda , Y. Klein , S. Bloch , H. Schwartz , E. Cohen , S. Shwartz

The authors present a review of the systematic studies of the structure of macroscopically planar thin films at the air-liquid interface (water, alkali solution and silica hydrosol). A common feature of the considered works is the…

In some super-resolution techniques, adjacent points are illuminated at different times. Thereby, their locations and light intensities can be detected even if the images are very blurred due to diffraction. According to conventional…

Image and Video Processing · Electrical Eng. & Systems 2019-12-10 Edward Y. Sheffield

We analyse mathematically the imaging modality using electromagnetic nanoparticles as contrast agent. This method uses the electromagnetic fields, collected before and after injecting electromagnetic nanoparticles, to reconstruct the…

Analysis of PDEs · Mathematics 2018-04-12 Durga Prasad Challa , Anupam Pal Choudhury , Mourad Sini

Imaging dynamical processes at interfaces and on the nanoscale is of great importance throughout science and technology. While light-optical imaging techniques often cannot provide the necessary spatial resolution, electron-optical…

Imaging of microvasculature is primarily performed with X-ray contrast agents, owing to the wide availability of absorption-contrast laboratory source microCT compared to phase contrast capable devices. Standard commercial contrast agents…

Tissues and Organs · Quantitative Biology 2023-01-18 Willy Kuo , Ngoc An Le , Bernhard Spingler , Georg Schulz , Bert Müller , Vartan Kurtcuoglu

One of the astounding consequences of quantum mechanics is that it allows the detection of a target using an incident probe, with only a low probability of interaction of the probe and the target. This 'quantum weirdness' could be applied…

Single-pixel imaging, originally developed in light optics, facilitates fast three-dimensional sample reconstruction, as well as probing with light wavelengths undetectable by conventional multi-pixel detectors. However, the spatial…

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