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Extending super-resolution imaging techniques to objects hidden in strongly scattering media potentially revolutionize the technical analysis for much broader categories of samples, such as biological tissues. The main challenge is the…

Unlike normal fluorescent methods that use the intensity as a direct measurement of the localized enhanced field, we use blinking statistics of quantum dots (QDs). We have already shown that blinking gives a more accurate characterization…

Chemical Physics · Physics 2017-11-02 Robert C. Boutelle , Xiyu Yi , Daniel Neuhauser , Shimon Weiss

We provide a quantitative analysis of super-resolution imaging techniques which exploit temporal fluctuations of luminosity of the sources in order to beat the Rayleigh limit. We define an operationally justified resolution gain figure of…

Quantum Physics · Physics 2021-06-08 Stanislaw Kurdzialek , Rafal Demkowicz-Dobrzanski

Fourier single-pixel imaging (FSI) has proven capable of reconstructing high-quality two-dimensional and three-dimensional images. The utilization of the sparsity of natural images in Fourier domain allows high-resolution images to be…

Computer Vision and Pattern Recognition · Computer Science 2016-12-12 Zibang Zhang , Xueying Wang , Jingang Zhong

Improving the resolution of fluorescence microscopy beyond the diffraction limit can be achievedby acquiring and processing multiple images of the sample under different illumination conditions.One of the simplest techniques, Random…

Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM…

We proposed a method to achieve superresolved optical imaging without beating the diffraction limit of light. This is achieved by magnifying the ideal optical image of the object through higher-order spatial frequency generation while…

Optics · Physics 2016-08-08 Zhixiang Li , Jianji Liu , Guoquan Zhang

Intensity correlation microscopy (ICM), which is prominently known through antibunching microscopy or super-resolution optical fluctuation imaging (SOFI), provides superresolution through a correlation analysis of antibunching of…

Optics · Physics 2018-10-08 Anton Classen , Joachim von Zanthier , Girish S. Agarwal

This paper introduces Spectral Incoherent Diffractive Imaging (SIDI) as a novel method for achieving dark-field imaging of nanostructures with heterogeneous oxidation states. With SIDI, shifts in photoemission profiles can be spatially…

Optics · Physics 2023-12-25 Tamme Wollweber , Kartik Ayyer

Super-resolution light microscopy overcomes the physical barriers due to light diffraction, allowing for the observation of otherwise indistinguishable subcellular entities. However, the specific acquisition conditions required by…

Optical imaging plays a critical role in advancing our understanding of three dimensional dynamics of biological systems. Coherent imaging (CI) methods exploit spatial phase information, encoded through propagation of coherent signal light…

Optics · Physics 2015-07-24 Jeffrey J. Field , David G. Winters , Randy A. Bartels

Abrupt fluorescence intermittency or blinking is long recognized to be characteristic of single nano-emitters. Extended quantum-confined nanostructures also undergo spatially heterogeneous blinking, however, there is no such precedence in…

Mesoscale and Nanoscale Physics · Physics 2018-08-27 Nithin Pathoor , Ansuman Halder , Amitrajit Mukherjee , Jaladhar Mahato , Shaibal K. Sarkar , Arindam Chowdhury

Confocal microscopy has long been a cornerstone technique for visualizing complex interactions and processes within cellular structures. However, achieving super-resolution imaging of multiple organelles and their interactions…

Optics · Physics 2025-08-19 Qinglin Chen , Luwei Wang , Jia Li , Dan Shao , Xiaoyu Weng , Liwei Liu , Dayong Jin , Junle Qu

Immunofluorescent (IF) imaging is crucial for visualizing biomarker expressions, cell morphology and assessing the effects of drug treatments on sub-cellular components. IF imaging needs extra staining process and often requiring cell…

Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…

Optics · Physics 2022-01-26 Yi Xue , David Ren , Laura Waller

We introduce a superresolution technique that combines spatial mode demultiplexing (SPADE) with emitter blinking. We show that temporal fluctuations not only enhance the precision of SPADE imaging, but also drastically simplify the…

Optics · Physics 2025-11-27 Stanislaw Kurdzialek

Super-resolved far-field microscopy has emerged as a powerful tool for investigating the structure of objects with resolution well below the diffraction limit of light. Nearly all super-resolution imaging techniques reported to date rely on…

Super-resolution localization microscopy is based on determining the positions of individual fluorescent markers in a sample. The major challenge in reaching an ever higher localization precision lies in the limited number of collected…

Optics · Physics 2020-08-05 Daniel Böning , Franz-Ferdinand Wieser , Vahid Sandoghdar

The diffraction of light imposes a fundamental limit on the resolution of light microscopes. This limit can be circumvented by creating and exploiting independent behaviors of the sample at length scales below the diffraction limit. In…

Biological Physics · Physics 2024-06-19 Mohamadreza Fazel , Michael J. Wester

Super-resolution imaging based on single molecule localization allows accessing nanometric-scale information in biological samples with high precision. However, complete measurements including molecule orientation are still challenging.…