Related papers: Spinning Disk -- Remote Focusing Microscopy
Fast, volumetric imaging over large scales has been a long-standing goal in biological microscopy. Scanning techniques such as fluorescence confocal microscopy can acquire 2D images at high resolution and high speed, but extending the…
Capturing biological specimens at large scales with sub-micron resolution is crucial for biomedical research, but conventional cameras often can't handle the pixel requirements. While most microscopes use motorized stages to move samples…
Magnonics, i.e. the artificial manipulation of spin waves, is a flourishing field of research with many potential uses in data processing within reach. Apart from the technological applications the possibility to directly influence and…
Imaging specimens over large scales and with a sub-micron resolution is instrumental to biomedical research. Yet, the number of pixels to form such an image usually exceeds the number of pixels provided by conventional cameras. While most…
Achieving fast, large-scale volumetric imaging with micrometer resolution has been a persistent challenge in the field of biological microscopy. To address this challenge, we report an augmented version of light field microscopy,…
Recent advances in optical technology have significantly enhanced the resolution of imaging of living cells, achieving nanometer-scale precision. However, the crowded three-dimensional environment within cells presents a challenge for…
Multifocus microscopy enables recording of entire volumes in a single camera exposure. In dense samples, multifocus microscopy is severely hampered by background haze. Here, we introduce a scalable multifocus method that incorporates…
Circular and linear zone plates have been fabricated on the surface of silicon crystals for the energy of 8 keV by electron beam lithography and deep ion plasma etching methods. Various variants of compound zone plates with first, second,…
Image segmentation in the medical domain is a challenging field owing to poor resolution and limited contrast. The predominantly used conventional segmentation techniques and the thresholding methods suffer from limitations because of heavy…
We demonstrate a simple and cost-efficient scanning confocal microscope setup for use in advanced instructional physics laboratories. The setup is constructed from readily available commercial products, and the implementation of a…
Today the gold standard for in vivo imaging through scattering tissue is the point-scanning two-photon microscope (PSTPM). Especially in neuroscience, PSTPM is widely used for deep-tissue imaging in the brain. However, due to sequential…
Purpose: In multi-spectral imaging (MSI), several fast spin echo volumes with discrete Larmor frequency offsets are acquired in an interleaved fashion with multiple concatenations. Here, a variable resolution (VR) method to nearly halve…
Intervertebral discs (IVDs), as small joints lying between adjacent vertebrae, have played an important role in pressure buffering and tissue protection. The fully-automatic localization and segmentation of IVDs have been discussed in the…
Accurate segmentation of the optic disc from a retinal image is vital to extracting retinal features that may be highly correlated with retinal conditions such as glaucoma. In this paper, we propose a deep-learning based approach capable of…
Corneal evaluation in ophthalmology necessitates cellular-resolution and fast imaging techniques allowing accurate diagnoses. Currently, the fastest volumetric imaging technique is Fourier-domain full-field optical coherence tomography…
We have developed and trained a convolutional neural network to automatically and simultaneously segment optic disc, fovea and blood vessels. Fundus images were normalised before segmentation was performed to enforce consistency in…
Dynamic axial focusing functionality has recently experienced widespread incorporation in microscopy, augmented/virtual reality (AR/VR), adaptive optics, and material processing. However, the limitations of existing varifocal tools continue…
Three-dimensional (3D) fluorescence microscopy in general requires axial scanning to capture images of a sample at different planes. Here we demonstrate that a deep convolutional neural network can be trained to virtually refocus a 2D…
Research and medicine rely on non-invasive optical techniques to image living tissue with high resolution in space and time. But so far a single data acquisition could not provide entirely diffraction-limited tomographic volumes of rapidly…
We developed a Bessel light sheet fluorescence microscopy (LSFM) system to enable high-speed, wide-field intra-vital imaging of zebrafish and other thick biological samples. This system uses air objectives for the convenient mounting of…