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We propose a cell segmentation method for analyzing images of densely clustered cells. The method combines the strengths of marker-controlled watershed transformation and a convolutional neural network (CNN). We demonstrate the method…

Image and Video Processing · Electrical Eng. & Systems 2020-04-06 Filip Lux , Petr Matula

Quantitative analysis of cell nuclei in microscopic images is an essential yet challenging source of biological and pathological information. The major challenge is accurate detection and segmentation of densely packed nuclei in images…

Quantitative Methods · Quantitative Biology 2019-11-14 Linqing Feng , Jun Ho Song , Jiwon Kim , Soomin Jeong , Jin Sung Park , Jinhyun Kim

The presented algorithms for segmentation and tracking follow a 3-step approach where we detect, track and finally segment nuclei. In the preprocessing phase, we detect centroids of the cell nuclei using a convolutional neural network (CNN)…

Computer Vision and Pattern Recognition · Computer Science 2019-04-16 Dennis Eschweiler , Johannes Stegmaier

We consider the problem of accurately identifying cell boundaries and labeling individual cells in confocal microscopy images, specifically, 3D image stacks of cells with tagged cell membranes. Precise identification of cell boundaries,…

Computer Vision and Pattern Recognition · Computer Science 2019-09-06 Jiaxiang Jiang , Po-Yu Kao , Samuel A. Belteton , Daniel B. Szymanski , B. S. Manjunath

Tracking of plant cells in images obtained by microscope is a challenging problem due to biological phenomena such as large number of cells, non-uniform growth of different layers of the tightly packed plant cells and cell division.…

Computer Vision and Pattern Recognition · Computer Science 2023-09-22 Md Shazid Islam , Arindam Dutta , Calvin-Khang Ta , Kevin Rodriguez , Christian Michael , Mark Alber , G. Venugopala Reddy , Amit K. Roy-Chowdhury

The prospect of neural reconstruction from Electron Microscopy (EM) images has been elucidated by the automatic segmentation algorithms. Although segmentation algorithms eliminate the necessity of tracing the neurons by hand, significant…

Computer Vision and Pattern Recognition · Computer Science 2015-09-29 Toufiq Parag

The objective of this study was to develop a PET tumor-segmentation framework that addresses the challenges of limited spatial resolution, high image noise, and lack of clinical training data with ground-truth tumor boundaries in PET…

Automated semantic segmentation of cell nuclei in microscopic images is crucial for disease diagnosis and tissue microenvironment analysis. Nonetheless, this task presents challenges due to the complexity and heterogeneity of cells. While…

Image and Video Processing · Electrical Eng. & Systems 2023-08-10 Zhuchen Shao , Sourya Sengupta , Hua Li , Mark A. Anastasio

Recent years have seen increasing use of supervised learning methods for segmentation tasks. However, the predictive performance of these algorithms depends on the quality of labels. This problem is particularly pertinent in the medical…

Computer Vision and Pattern Recognition · Computer Science 2020-10-26 Le Zhang , Ryutaro Tanno , Mou-Cheng Xu , Chen Jin , Joseph Jacob , Olga Ciccarelli , Frederik Barkhof , Daniel C. Alexander

Nuclei instance segmentation on histopathology images is of great clinical value for disease analysis. Generally, fully-supervised algorithms for this task require pixel-wise manual annotations, which is especially time-consuming and…

Computer Vision and Pattern Recognition · Computer Science 2023-06-06 Yang Zhou , Yongjian Wu , Zihua Wang , Bingzheng Wei , Maode Lai , Jianzhong Shou , Yubo Fan , Yan Xu

Deep learning-based nuclei segmentation and classification in pathology images typically rely on large-scale pixel-level manual annotations, which are costly and difficult to obtain across diverse tissues and staining conditions. To address…

Computer Vision and Pattern Recognition · Computer Science 2026-04-28 Kazuya Nishimura , Ryoma Bise , Haruka Hirose , Yasuhiro Kojima

Automatic segmentation of microscopy images is an important task in medical image processing and analysis. Nucleus detection is an important example of this task. Mask-RCNN is a recently proposed state-of-the-art algorithm for object…

Computer Vision and Pattern Recognition · Computer Science 2021-04-01 Jeremiah W. Johnson

Nuclei appear small in size, yet, in real clinical practice, the global spatial information and correlation of the color or brightness contrast between nuclei and background, have been considered a crucial component for accurate nuclei…

Image and Video Processing · Electrical Eng. & Systems 2023-07-18 Zhenqi He , Mathias Unberath , Jing Ke , Yiqing Shen

Despite their superior performance, deep-learning methods often suffer from the disadvantage of needing large-scale well-annotated training data. In response, recent literature has seen a proliferation of efforts aimed at reducing the…

Computer Vision and Pattern Recognition · Computer Science 2023-07-12 Ji Yu

We present our novel deep multi-task learning method for medical image segmentation. Existing multi-task methods demand ground truth annotations for both the primary and auxiliary tasks. Contrary to it, we propose to generate the…

Image and Video Processing · Electrical Eng. & Systems 2022-04-06 Binod Bhattarai , Ronast Subedi , Rebati Raman Gaire , Eduard Vazquez , Danail Stoyanov

Semantic segmentation of microscopic cell images using deep learning is an important technique, however, it requires a large number of images and ground truth labels for training. To address the above problem, we consider an efficient…

Computer Vision and Pattern Recognition · Computer Science 2023-04-18 Sota Kato , Kazuhiro Hotta

Microscopy structure segmentation, such as detecting cells or nuclei, generally requires a human to draw a ground truth contour around each instance. Weakly supervised approaches (e.g. consisting of only single point labels) have the…

Computer Vision and Pattern Recognition · Computer Science 2024-11-25 James Willoughby , Irina Voiculescu

We share our recent findings in an attempt to train a universal segmentation network for various cell types and imaging modalities. Our method was built on the generalized U-Net architecture, which allows the evaluation of each component…

Computer Vision and Pattern Recognition · Computer Science 2022-08-01 Tianqi Guo , Yin Wang , Luis Solorio , Jan P. Allebach

Due to the wide existence and large morphological variances of nuclei, accurate nuclei instance segmentation is still one of the most challenging tasks in computational pathology. The annotating of nuclei instances, requiring experienced…

Computer Vision and Pattern Recognition · Computer Science 2020-07-23 Xinpeng Xie , Jiawei Chen , Yuexiang Li , Linlin Shen , Kai Ma , Yefeng Zheng

Over the last decade, electron microscopy has improved up to a point that generating high quality gigavoxel sized datasets only requires a few hours. Automated image analysis, particularly image segmentation, however, has not evolved at the…

Computer Vision and Pattern Recognition · Computer Science 2019-11-14 Joris Roels , Yvan Saeys