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Accurate segmentation of 3-D cell nuclei in microscopy images is essential for the study of nuclear organization, gene expression, and cell morphodynamics. Current image segmentation methods are challenged by the complexity and variability…

Image and Video Processing · Electrical Eng. & Systems 2022-03-18 Sundaresh Ram , Jeffrey J. Rodriguez

We propose a 3D convolutional neural network to simultaneously segment and detect cell nuclei in confocal microscopy images. Mirroring the co-dependency of these tasks, our proposed model consists of two serial components: the first part…

Image and Video Processing · Electrical Eng. & Systems 2018-09-07 Sundaresh Ram , Vicky T. Nguyen , Kirsten H. Limesand , Mert R. Sabuncu

Cell nuclei segmentation is one of the most important tasks in the analysis of biomedical images. With ever-growing sizes and amounts of three-dimensional images to be processed, there is a need for better and faster segmentation methods.…

Computer Vision and Pattern Recognition · Computer Science 2017-02-20 Julian Arz , Peter Sanders , Johannes Stegmaier , Ralf Mikut

Background Analyzing images to accurately estimate the number of different cell types in the brain using automatic methods is a major objective in neuroscience. The automatic and selective detection and segmentation of neurons would be an…

Image and Video Processing · Electrical Eng. & Systems 2024-10-07 Antonio LaTorre , Lidia Alonso-Nanclares , José María Peña , Javier De Felipe

Advances in fluorescence microscopy enable acquisition of 3D image volumes with better image quality and deeper penetration into tissue. Segmentation is a required step to characterize and analyze biological structures in the images and…

Computer Vision and Pattern Recognition · Computer Science 2019-04-16 Chichen Fu , Soonam Lee , David Joon Ho , Shuo Han , Paul Salama , Kenneth W. Dunn , Edward J. Delp

Every year millions of people die due to disease of Cancer. Due to its invasive nature it is very complex to cure even in primary stages. Hence, only method to survive this disease completely is via forecasting by analyzing the early…

Computer Vision and Pattern Recognition · Computer Science 2018-08-24 Shivam Singh , Stuti Pathak

We propose a novel weakly supervised method to improve the boundary of the 3D segmented nuclei utilizing an over-segmented image. This is motivated by the observation that current state-of-the-art deep learning methods do not result in…

Computer Vision and Pattern Recognition · Computer Science 2021-08-31 S. Shailja , Jiaxiang Jiang , B. S. Manjunath

The need for labour intensive pixel-wise annotation is a major limitation of many fully supervised learning methods for segmenting bioimages that can contain numerous object instances with thin separations. In this paper, we introduce a…

Computer Vision and Pattern Recognition · Computer Science 2020-09-11 Rihuan Ke , Aurélie Bugeau , Nicolas Papadakis , Peter Schuetz , Carola-Bibiane Schönlieb

Nuclei segmentation is a crucial task for whole slide image analysis in digital pathology. Generally, the segmentation performance of fully-supervised learning heavily depends on the amount and quality of the annotated data. However, it is…

Image and Video Processing · Electrical Eng. & Systems 2023-08-21 Yi Lin , Zhiyong Qu , Hao Chen , Zhongke Gao , Yuexiang Li , Lili Xia , Kai Ma , Yefeng Zheng , Kwang-Ting Cheng

Fully supervised deep neural networks for segmentation usually require a massive amount of pixel-level labels which are manually expensive to create. In this work, we develop a multi-task learning method to relax this constraint. We regard…

Computer Vision and Pattern Recognition · Computer Science 2021-04-07 Rihuan Ke , Aurélie Bugeau , Nicolas Papadakis , Mark Kirkland , Peter Schuetz , Carola-Bibiane Schönlieb

Nuclei segmentation is a fundamental task in histopathology image analysis. Typically, such segmentation tasks require significant effort to manually generate accurate pixel-wise annotations for fully supervised training. To alleviate such…

Computer Vision and Pattern Recognition · Computer Science 2020-07-13 Hui Qu , Pengxiang Wu , Qiaoying Huang , Jingru Yi , Zhennan Yan , Kang Li , Gregory M. Riedlinger , Subhajyoti De , Shaoting Zhang , Dimitris N. Metaxas

Nuclei segmentation is a fundamental prerequisite in the digital pathology workflow. The development of automated methods for nuclei segmentation enables quantitative analysis of the wide existence and large variances in nuclei morphometry…

Computer Vision and Pattern Recognition · Computer Science 2024-01-17 Yi Lin , Zeyu Wang , Dong Zhang , Kwang-Ting Cheng , Hao Chen

Fluorescence microscopy is an essential tool for the analysis of 3D subcellular structures in tissue. An important step in the characterization of tissue involves nuclei segmentation. In this paper, a two-stage method for segmentation of…

Image and Video Processing · Electrical Eng. & Systems 2020-01-10 David Joon Ho , Shuo Han , Chichen Fu , Paul Salama , Kenneth W. Dunn , Edward J. Delp

We propose a software platform that integrates methods and tools for multi-objective parameter auto- tuning in tissue image segmentation workflows. The goal of our work is to provide an approach for improving the accuracy of nucleus/cell…

Distributed, Parallel, and Cluster Computing · Computer Science 2018-10-09 Luis F. R. Taveira , Tahsin Kurc , Alba C. M. A. Melo , Jun Kong , Erich Bremer , Joel H. Saltz , George Teodoro

AI-assisted nuclei segmentation in histopathological images is a crucial task in the diagnosis and treatment of cancer diseases. It decreases the time required to manually screen microscopic tissue images and can resolve the conflict…

Image and Video Processing · Electrical Eng. & Systems 2023-11-21 Hesham Ali , Idriss Tondji , Mennatullah Siam

Detecting and segmenting individual cells from microscopy images is critical to various life science applications. Traditional cell segmentation tools are often ill-suited for applications in brightfield microscopy due to poor contrast and…

Image and Video Processing · Electrical Eng. & Systems 2020-05-20 Rituparna Sarkar , Suvadip Mukherjee , Elisabeth Labruyère , Jean-Christophe Olivo-Marin

Though performed almost effortlessly by humans, segmenting 2D gray-scale or color images into respective regions of interest (e.g.~background, objects, or portions of objects) constitutes one of the greatest challenges in science and…

Computer Vision and Pattern Recognition · Computer Science 2023-08-23 Alexandre Benatti , Luciano da F. Costa

Nuclei segmentation is a fundamental but challenging task in the quantitative analysis of histopathology images. Although fully-supervised deep learning-based methods have made significant progress, a large number of labeled images are…

Image and Video Processing · Electrical Eng. & Systems 2024-01-22 Xinyi Yu , Guanbin Li , Wei Lou , Siqi Liu , Xiang Wan , Yan Chen , Haofeng Li

Nuclei segmentation is one of the important tasks for whole slide image analysis in digital pathology. With the drastic advance of deep learning, recent deep networks have demonstrated successful performance of the nuclei segmentation task.…

Computer Vision and Pattern Recognition · Computer Science 2019-07-23 Inwan Yoo , Donggeun Yoo , Kyunghyun Paeng

Nuclei segmentation is a fundamental task in digital pathology analysis and can be automated by deep learning-based methods. However, the development of such an automated method requires a large amount of data with precisely annotated masks…

Computer Vision and Pattern Recognition · Computer Science 2021-11-25 Jiangbo Shi , Chang Jia , Zeyu Gao , Tieliang Gong , Chunbao Wang , Chen Li
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