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Cryo-electron microscopy (cryo-EM) is an imaging modality that provides unique insights into the dynamics of proteins and other building blocks of life. The algorithmic challenge of jointly estimating the poses, 3D structure, and…

Computer Vision and Pattern Recognition · Computer Science 2022-10-17 Axel Levy , Gordon Wetzstein , Julien Martel , Frederic Poitevin , Ellen D. Zhong

We present a reconstruction method involving maximum-likelihood expectation maximization (MLEM) to model Poisson noise as applied to fluorescence molecular tomography (FMT). MLEM is initialized with the output from a sparse…

Medical Physics · Physics 2018-04-03 Yansong Zhu , Abhinav K. Jha , Dean F. Wong , Arman Rahmim

Image restoration aims to restore high-quality images from degraded counterparts and has seen significant advancements through deep learning techniques. The technique has been widely applied to mobile devices for tasks such as mobile…

Computer Vision and Pattern Recognition · Computer Science 2023-08-25 Xiangyu Chen , Ruiwen Zhen , Shuai Li , Xiaotian Li , Guanghui Wang

Magnetic materials phase reconstruction from Lorentz transmission electron microscopy (LTEM) measurements has traditionally been achieved using longstanding methods such as off-axis holography (OAH) and the transport-of-intensity equation…

Limited-angle electron tomography aims to reconstruct 3D shapes from 2D projections of Transmission Electron Microscopy (TEM) within a restricted range and number of tilting angles, but it suffers from the missing-wedge problem that causes…

Computer Vision and Pattern Recognition · Computer Science 2026-05-05 Zhantao Deng , Mériem Er-Rafik , Anna Sushko , Cécile Hébert , Pascal Fua

We study the increase of image noise which can be observed in images reconstructed with the OSEM algorithm. We argue that the excessive noise is equivalent to shortening scanning time by a factor of 2 or more at high number of subsets…

Medical Physics · Physics 2018-07-02 Krzysztof Kacperski , Dominika Świtlik , Jakub Pietrzak

The evolution of the scanning modules for scanning transmission electron microscopes (STEM) has realized the possibility to generate arbitrary scan pathways, an approach currently explored to improve acquisition speed and to reduce electron…

The Cryo-EM 3D particle reconstruction is essential for identifying protein and uncover the biological mechanism of the macro-molecules. In this paper, we use Kam method for reconstruction. Kam method is \textit{ab-initio}, and it assumes…

Quantitative Methods · Quantitative Biology 2019-06-21 Yin Xian

Efficient and fast reconstruction of anatomical structures plays a crucial role in clinical practice. Minimizing retrieval and processing times not only potentially enhances swift response and decision-making in critical scenarios but also…

A detailed analysis of ptychography for 3D phase reconstructions of thick specimens is performed. We introduce multi-focus ptychography, which incorporates a 4D-STEM defocus series to enhance the quality of 3D reconstructions along the beam…

Direct reconstruction of positron emission tomography (PET) data using deep neural networks is a growing field of research. Initial results are promising, but often the networks are complex, memory utilization inefficient, produce…

Image and Video Processing · Electrical Eng. & Systems 2020-06-16 William Whiteley , Vladimir Panin , Chuanyu Zhou , Jorge Cabello , Deepak Bharkhada , Jens Gregor

Multislice electron ptychography (MEP) is an inverse imaging technique that computationally reconstructs the highest-resolution images of atomic crystal structures from diffraction patterns. Available algorithms often solve this inverse…

Image and Video Processing · Electrical Eng. & Systems 2025-07-28 Christian K. Belardi , Chia-Hao Lee , Yingheng Wang , Justin Lovelace , Kilian Q. Weinberger , David A. Muller , Carla P. Gomes

The image reconstruction of partially coherent light is interpreted as the quantum state reconstruction. The efficient method based on maximum-likelihood estimation is proposed to acquire information from registered intensity measurements…

Optics · Physics 2011-07-22 M. Jezek , Z. Hradil

The most established method of reconstructing neural circuits from animals involves slicing tissue very thin, then taking mosaics of electron microscope (EM) images. To trace neurons across different images and through different sections,…

Quantitative Methods · Quantitative Biology 2013-04-23 Louis K. Scheffer , Bill Karsh , Shiv Vitaladevun

Transmission Electron Microscopy (TEM) is a powerful tool for imaging material structure and characterizing material chemistry. Recent advances in data collection technology for TEM have enabled high-volume and high-resolution data…

Computer Vision and Pattern Recognition · Computer Science 2021-08-24 Josh Kacher , Yao Xie , Sven P. Voigt , Shixiang Zhu , Henry Yuchi , Jordan Key , Surya R. Kalidindi

A major challenge in computed tomography (CT) is to reduce X-ray dose to a low or even ultra-low level while maintaining the high quality of reconstructed images. We propose a new method for CT reconstruction that combines penalized…

Machine Learning · Statistics 2017-07-11 Xuehang Zheng , Zening Lu , Saiprasad Ravishankar , Yong Long , Jeffrey A. Fessler

Accurate signal recovery or image reconstruction from indirect and possibly undersampled data is a topic of considerable interest; for example, the literature in the recent field of compressed sensing is already quite immense. Inspired by…

Optimization and Control · Mathematics 2011-04-15 Stephen Becker , Jerome Bobin , Emmanuel Candes

Significant progress has been made in spatial resolution using environmental transmission electron microscopes (ETEM), which now enables atomic resolution visualization of structural transformation under variable temperature and gas…

Materials Science · Physics 2015-06-18 Wenpei Gao , Jianbo Wu , Xiaofeng Zhang , Aram Yoon , J. Mabon , W. Swiech , W. L. Wilson , H. Yang , Jian-Min Zuo

Image simulation for scanning transmission electron microscopy at atomic resolution for samples with realistic dimensions can require very large computation times using existing simulation algorithms. We present a new algorithm named PRISM…

Materials Science · Physics 2017-04-24 Colin Ophus

Simulation of atomic resolution image formation in scanning transmission electron microscopy can require significant computation times using traditional methods. A recently developed method, termed plane-wave reciprocal-space interpolated…

Computational Physics · Physics 2017-07-07 Alan Pryor , Colin Ophus , Jianwei Miao