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Super-resolution fluorescence microscopy overcomes blurring arising from light diffraction, allowing the reconstruction of fine scale details in biological structures. Standard methods come at the expense of long acquisition time and/or…

Optimization and Control · Mathematics 2021-10-12 Bastien Laville , Laure Blanc-Féraud , Gilles Aubert

To overcome the limit of diffraction while achieving the superresolution technique, solid immersion lenses are the key optical elements for data storage and nanophotonics applications. Recent demonstrations have shown how different…

Optics · Physics 2026-02-24 Subhankar Roy , Jianping Hu , M Ummal Momeen

We demonstrate a new computational illumination technique that achieves large space-bandwidth-time product, for quantitative phase imaging of unstained live samples in vitro. Microscope lenses can have either large field of view (FOV) or…

Optics · Physics 2015-10-16 Lei Tian , Ziji Liu , Li-Hao Yeh , Michael Chen , Jingshan Zhong , Laura Waller

Image Phase Alignment Super-sampling (ImPASS) is a computational method for combining displaced low-resolution images into a single high-resolution image. The general steps include measuring the relative displacements, up-sampling, aligning…

Optics · Physics 2026-01-09 James N. Caron

The Ocean Color Monitor-3 (OCM-3) sensor is instrumental in Earth observation, achieving a critical balance between high-resolution imaging and broad coverage. This paper explores innovative imaging methods employed in OCM-3 and the…

Image and Video Processing · Electrical Eng. & Systems 2024-10-25 Ankur Garg , Tushar Shukla , Purvee Joshi , Debojyoti Ganguly , Ashwin Gujarati , Meenakshi Sarkar , KN Babu , Mehul Pandya , S. Manthira Moorthi , Debajyoti Dhar

Developing a chip-based super-resolution imaging technique with large field-of-view (FOV), deep subwavelength resolution, and compatibility for both fluorescent and non-fluorescent samples is desired for material science, biomedicine, and…

We present experimental demonstration of tilt-mirror assisted transmission structured illumination microscopy (tSIM) that offers a large field of view super resolution imaging. An assembly of custom-designed tilt-mirrors are employed as the…

Super-resolution microscopy has revolutionized optical fluorescence imaging by improving 3D resolution by 1-2 orders of magnitude. While different methods can successfully increase the resolution, all methods share significant differences…

Biological Physics · Physics 2015-01-26 Thomas Pengo , Nicolas Olivier , Suliana Manley

Confocal fluorescence microscopy is one of the most accessible and widely used imaging techniques for the study of biological processes at the cellular and subcellular levels. Scanning confocal microscopy allows the capture of high-quality…

Image and Video Processing · Electrical Eng. & Systems 2024-11-01 Soufiane Belharbi , Mara KM Whitford , Phuong Hoang , Shakeeb Murtaza , Luke McCaffrey , Eric Granger

Structured illumination microscopy (SIM) is a very important super-resolution microscopy technique, which provides high speed super-resolution with about two-fold spatial resolution enhancement. Several attempts aimed at improving the…

Computer Vision and Pattern Recognition · Computer Science 2016-02-23 Amit Lal , Chunyan Shan , Peng Xi

Digital holographic microscopy (DHM) applied to quantitative phase imaging (QPI) has been successfully demonstrated as a powerful label-free method to analyse the optical properties of cells. Spatially multiplexed interferometric microscopy…

Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

Optics · Physics 2015-10-27 Jeffrey J. Field , David G. Winters , Randy A. Bartels

An integrated microscope that combines different optical techniques for simultaneous imaging is demonstrated. The microscope enables spectral-domain optical coherence microscopy based on optical backscatter, and multi-photon microscopy for…

Optics · Physics 2009-11-11 C. Vinegoni , T. Ralston , W. Tan , W. Luo , D. L. Marks , S. A. Boppart

We propose to enhance the performance of localized plasmon structured illumination microscopy (LP-SIM) via intensity correlations. LP-SIM uses sub-wavelength illumination patterns to encode high spatial frequency information. It can enhance…

Scanning electron microscopy (SEM) is indispensable in diverse applications ranging from microelectronics to food processing because it provides large depth-of-field images with a resolution beyond the optical diffraction limit. However,…

Selective plane illumination microscopy (SPIM) is an optical sectioning imaging approach based on orthogonal light pathways for excitation and detection. The excitation pathway has an inverse relation between the optical sectioning strength…

Optics · Physics 2024-07-29 Steven J. Sheppard , Peter T. Brown , Douglas P. Shepherd

This thesis centres on the development of multidimensional fluorescence imaging tools, with a particular emphasis on fluorescence lifetime imaging (FLIM) microscopy for application to biological research. The key aspects of this thesis are…

Instrumentation and Detectors · Physics 2017-08-14 Egidijus Auksorius

Monolayer semiconductors hold great potential for nanoscale electronics, optoelectronics, and photonics. Excitons dominate their optical properties. As their electric fields extend outside the monolayer, they are sensitive to their…

Fluorescence imaging is an essential diagnostic tool in many fields, but diffraction-limited optical imaging at depth is limited by scattering. Here, we present a method based on multiple random illuminations, combined with a computational…

Optics · Physics 2026-03-30 Lei Zhu , Tengfei Wu , Bernhard Rauer , Hilton B. de Aguiar , Sylvain Gigan

Optical scattering presents a major obstacle to high resolution imaging in biological tissue and other turbid media. Conventional photoacoustic imaging can partially overcome this obstacle, enabling imaging of optical absorption in the…

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