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Super-resolution structured illumination microscopy (SR-SIM) is a widely used technique for enhancing the resolution of fluorescence imaging beyond the diffraction limit. Most existing SR-SIM methods rely on Moir\'e effect-based physical…

Optics · Physics 2025-02-27 Junzheng Peng , Jiahao Xian , Xi Lin , Manhong Yao , Shiping Li , Jingang Zhong

We proposed a method to achieve superresolved optical imaging without beating the diffraction limit of light. This is achieved by magnifying the ideal optical image of the object through higher-order spatial frequency generation while…

Optics · Physics 2016-08-08 Zhixiang Li , Jianji Liu , Guoquan Zhang

Fluorescence lifetime imaging microscopy (FLIM) is a powerful quantitative technique that provides metabolic and molecular contrast, offering strong translational potential for label-free, real-time diagnostics. However, its clinical…

Computer Vision and Pattern Recognition · Computer Science 2025-12-19 Paloma Casteleiro Costa , Parnian Ghapandar Kashani , Xuhui Liu , Alexander Chen , Ary Portes , Julien Bec , Laura Marcu , Aydogan Ozcan

High-content biological microscopy targets high-resolution imaging across large fields-of-view (FOVs). Recent works have demonstrated that computational imaging can provide efficient solutions for high-content microscopy. Here, we use…

Image and Video Processing · Electrical Eng. & Systems 2019-03-28 Li-Hao Yeh , Shwetadwip Chowdhury , Laura Waller

Image Phase Alignment Super-Sampling (ImPASS) is a computational imaging algorithm for converting a sequence of displaced low-resolution images into a single high-resolution image. The method consists of a unique combination of Phase…

Optics · Physics 2025-09-01 James N. Caron

Extending super-resolution imaging techniques to objects hidden in strongly scattering media potentially revolutionize the technical analysis for much broader categories of samples, such as biological tissues. The main challenge is the…

Fourier ptychography microscopy (FPM), sharing its roots with synthetic aperture technique and phase retrieval method, is a recently developed computational microscopic super-resolution technique. By turning on the light-emitting diode…

Optics · Physics 2021-05-07 G. Zhou , S. Zhang , C. Zheng , T. Li , Y. Hu , Q. Hao

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going "beyond the diffraction barrier" comes at a price since most far-field super-resolution imaging techniques…

Optical diffraction tomography is an indispensable tool for studying objects in three-dimensions due to its ability to accurately reconstruct scattering objects. Until now this technique has been limited to coherent light because spatial…

One of the fundamental limits of classical optical microscopy is the diffraction limit of optical resolution. It results from the finite bandwidth of the optical transfer function (or OTF) of an optical microscope, which restricts the…

Optics · Physics 2022-03-11 Sjoerd Stallinga , Niels Radmacher , Antoine Delon , Jörg Enderlein

We numerically investigate the role of quantum fluctuations in superresolution of optical objects. First, we confirm that when quantum fluctuations are not taken into account, one can easily improve the resolution by one order of magnitude…

Quantum Physics · Physics 2007-05-23 Vladislav N. Beskrovnyy , Mikhail I. Kolobov

We propose to use intensity correlation microscopy in combination with structured illumination to image quantum emitters that exhibit antibunching with a spatial resolution reaching far beyond the Rayleigh limit. Combining intensity…

Diffraction unlimited super-resolution imaging critically depends on the switching of fluorophores between at least two states, often induced using intense laser light and special buffers. The high illumination power or UV light required…

We introduce a superresolution technique that combines spatial mode demultiplexing (SPADE) with emitter blinking. We show that temporal fluctuations not only enhance the precision of SPADE imaging, but also drastically simplify the…

Optics · Physics 2025-11-27 Stanislaw Kurdzialek

Imaging in thick biological tissues is often degraded by sample-induced aberrations, which reduce image quality and resolution, particularly in super-resolution techniques. While hardware-based adaptive optics, which correct aberrations…

We propose a 3D super-resolution approach to improve both lateral and axial spatial resolution in Total Internal Reflectance Fluorescence (TIRF) imaging applications. Our approach, called 3D-COL0RME (3D - Covariance-based $\ell_0$…

Medical Physics · Physics 2022-07-12 Vasiliki Stergiopoulou , Luca Calatroni , Sébastien Schaub , Laure Blanc-Féraud

In recent years, image-scanning microscopy (ISM, also termed pixel-reassignment microscopy) has emerged as a technique that improves the resolution and signal-to-noise compared to confocal and widefield microscopy by employing a detector…

Optics · Physics 2023-04-19 Tal I. Sommer , Gil Weinberg , Ori Katz

With the rapid development of spaceborne imaging techniques, object detection in optical remote sensing imagery has drawn much attention in recent decades. While many advanced works have been developed with powerful learning algorithms, the…

Computer Vision and Pattern Recognition · Computer Science 2019-12-19 Xin Wu , Danfeng Hong , Jiaojiao Tian , Jocelyn Chanussot , Wei Li , Ran Tao

Wide-field fluorescence microscopy, while much faster than confocal microscopy, suffers from a lack of optical sectioning and poor axial resolution. 3D structured illumination microscopy (SIM) has been demonstrated to provide optical…

Far-field optical microscopy using focused light is an important tool in a number of scientific disciplines including chemical, (bio)physical and biomedical research, particularly with respect to the study of living cells and organisms.…