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We introduce the basic elements of a spatio-angular theory of fluorescence microscopy, providing a unified framework for analyzing systems that image single fluorescent dipoles and ensembles of overlapping dipoles that label biological…

Fluorescent molecules are versatile nanoscale emitters that enable detailed observations of biophysical processes with nanoscale resolution. Because they are well-approximated as electric dipoles, imaging systems can be designed to…

Optics · Physics 2024-03-26 Tingting Wu , Matthew D. Lew

We study resonant response of an underdamped nanomechanical resonator with fluctuating frequency. The fluctuations are due to diffusion of molecules or microparticles along the resonator. They lead to broadening and change of shape of the…

Mesoscale and Nanoscale Physics · Physics 2013-05-29 J. Atalaya , A. Isacsson , M. I. Dykman

Optical fluorescence imaging is capable of measuring both the spatial and rotational dynamics of single molecules. However, unavoidable measurement noise will result in inaccurate estimates of rotational dynamics, causing a molecule to…

Optics · Physics 2019-05-22 Oumeng Zhang , Matthew D. Lew

We prove that it is impossible to distinguish two spatially coinciding fluorescent molecules from a single rotating molecule using polarization-sensitive imaging, even if one modulates the polarization of the illumination or the detection…

Optics · Physics 2025-03-11 Yiyang Chen , Yuanxin Qiu , Matthew D. Lew

We present an experimental realization of a biased optical periodic potential in the low friction limit. The noise-induced bistability between locked (torsional) and running (spinning) states in the rotational motion of a nanodumbbell is…

An optical field will undergo coherent diffusion when it is mapped into thermal-motioned atoms, e.g., in a slow or storage light process. As was demonstrated before, such diffusion effect is equivalent to a spatial low-pass filter…

Quantum Physics · Physics 2022-07-15 Yun Chen , Chengyuan Wang , Zibin Jiang , Wei Zhang , Zehao Shen , Hong Gao , Fuli Li

We investigate the impact of rotational diffusion on the electrodynamic coupling of fluorescent dye molecules (oscillating electric dipoles) to a tunable planar metallic nanocavity. Fast rotational diffusion of the molecules leads to a…

Brownian diffusion of rod-like polymers in the presence of randomly distributed spherical obstacles is studied using molecular dynamics (MD) simulations. It is observed that dependence of the reduced diffusion coefficient of these…

Soft Condensed Matter · Physics 2015-05-20 Farzaneh Sakha , Hossein Fazli

The expansion of viewing angle is a crucial factor in holographic displays implemented with a spatial light modulator having a finite space-bandwidth. The enhanced-NA Fresnel hologram reconstructs a holographic image at an angle larger than…

Optics · Physics 2023-08-24 Byung Gyu Chae

The enhanced-NA Fresnel hologram reconstructs a holographic image at a viewing angle larger than the diffraction angle of a hologram pixel. The image space is limited by the bandwidth of a digital hologram. In this study, we investigate the…

Optics · Physics 2022-06-28 Byung Gyu Chae

We consider anomalous diffusion for molecular communication with a passive receiver. We first consider the probability density function of molecules' location at a given time in a space of arbitrary dimension. The expected number of…

Information Theory · Computer Science 2021-07-09 Dung Phuong Trinh , Youngmin Jeong , Sang-Hyo Kim

We demonstrate that the emission of light by fluorescent molecules in the proximity of periodic arrays of nanoantennas or plasmonic crystals can be strongly modified when the arrays are covered by a dielectric film. The coupling between…

Optics · Physics 2009-01-14 G. Vecchi , V. Giannini , J. Gómez Rivas

We investigate the properties of a single-view fluorescence microscope in a 4$f$ geometry when imaging fluorescent dipoles without using the monopole or scalar approximations. We show that this imaging system has a spatio-angular band…

Fluorescent molecules emit light in a dipole radiation pattern that can be used to infer their orientation through defocused fluorescence microscopy. Proper measurement of the orientation requires mathematical modeling of the radiation…

Optics · Physics 2025-08-07 E. Dey , M. Elorza , F. W. Foss , J. J. Gomez Cadenas , B. J. P. Jones

We measure all nonzero elements of the three-dimensional (3D) diffusion tensor D for clusters of colloidal spheres to a precision of 1% or better using digital holographic microscopy. We study both dimers and triangular trimers of spheres,…

Soft Condensed Matter · Physics 2013-10-17 Jerome Fung , Vinothan N. Manoharan

Focusing control of ultrashort pulsed beams is an important research topic, due to its impact to subsequent interaction with matter. In this work, we study the propagation near the focus of ultrashort laser pulses of ~25 fs duration under…

Spectral properties of fluorescent radiation from a two-level quantum system with broken inversion spatial symmetry, which can be described by a model of an one-electron two-level atom whose electric dipole moment operator has permanent…

Quantum Physics · Physics 2024-12-17 Nikolai N. Bogolyubov, , Andrey V. Soldatov

Fractional, anomalous diffusion in space-periodic potentials is investigated. The analytical solution for the effective, fractional diffusion coefficient in an arbitrary periodic potential is obtained in closed form in terms of two…

Statistical Mechanics · Physics 2021-02-02 E. Heinsalu , M. Patriarca , I. Goychuk , P. Hanggi

Diffusion of tracer dye molecules in water confined to nanoscale is an important subject with a direct bearing on many technological applications. It is not yet clear however, if the dynamics of water in hydrophilic as well as hydrophobic…

Soft Condensed Matter · Physics 2022-01-19 Shivprasad Patil , V. J. Ajith
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