Related papers: Fully refractive adaptive optics fluorescence micr…
Adaptive optics in combination with multi-photon techniques is a powerful approach to image deep into a specimen. Remarkably, virtually all adaptive optics schemes today rely on wavefront modulators which are reflective, diffractive, or…
The resolution and contrast of microscope imaging is often affected by aberrations introduced by imperfect optical systems and inhomogeneous refractive structures in specimens. Adaptive optics (AO) compensates these aberrations and restores…
Three-photon (3-P) fluorescence microscopy enables deep in vivo imaging with subcellular resolution, but its performance is fundamentally constrained by the maximum permissible laser power required to avoid tissue heating and photodamage.…
Images obtained by microscopes are generally degraded by aberrations. Adaptive optics (AO) has been widely used in to compensate for aberrations and improve reduced image quality. Requiring no separate wavefront sensor, sensorless AO…
Future large space telescopes will be equipped with adaptive optics (AO) to overcome wavefront aberrations and achieve high contrast for imaging faint astronomical objects, such as earth-like exoplanets and debris disks. In contrast to AO…
Adaptive optics (AO) offers an opportunity to stabilize an image and maximize the spatial resolution achievable by ground based telescopes by removing the distortions due to the atmosphere. Typically, the deformable mirror in an AO system…
Astronomical telescopes suffer from a tradeoff between field of view (FoV) and image resolution: increasing the FoV leads to an optical field that is under-sampled by the science camera. This work presents a novel computational imaging…
Adaptive optics (AO) is critical in astronomy, optical communications and remote sensing to deal with the rapid blurring caused by the Earth's turbulent atmosphere. But current AO systems are limited by their wavefront sensors, which need…
Diffraction limited resolution adaptive optics (AO) correction in visible wavelengths requires a high performance control. In this paper we investigate infinite impulse response filters that optimize the wavefront correction: we tested…
The imaging performance of an optical microscope can be degraded by sample-induced aberrations. A general strategy to undo the effect of these aberrations is to apply wavefront correction with a deformable mirror (DM). In most cases, the DM…
Multifocal plane microscopy (MUM) allows three dimensional objects to be imaged in a single camera frame. Our approach uses dual orthogonal diffraction phase gratings with a quadratic distortion of the lines to apply defocus to the first…
The aberrations induced by the sample and/or by the sample holder limit the resolution of optical microscopes. Wavefront correction can be achieved using a deformable mirror with wavefront sensorless optimization algorithms but, despite the…
Adaptive optics (AO) has revolutionized imaging in {fields} from astronomy to microscopy by correcting optical aberrations. In label-free microscopes, however, conventional AO faces limitations due to the absence of guidestar and the need…
Context. Adaptive optics (AO) is now a tool commonly deployed in astronomy. The real time correction of the atmospheric turbulence that AO enables allows telescopes to perform close to the diffraction limit at the core of their point spread…
The pyramid wavefront sensor (P-WFS) has replaced the Shack-Hartmann (SH-) WFS as sensor of choice for high performance adaptive optics (AO) systems in astronomy because of its flexibility in pupil sampling, its dynamic range, and its…
Light microscopy enables multifunctional imaging of biological specimens at unprecedented depths and resolutions. However, the performance of all optical methods degrades with the imaging depth due to sample-induced aberrations. Methods of…
Due to turbulence in the atmosphere images taken from ground-based telescopes become distorted. With adaptive optics (AO) images can be given greater clarity allowing for better observations with existing telescopes and are essential for…
The main objective of the present project is to explore the viability of an adaptive optics control system based exclusively on Field Programmable Gate Arrays (FPGAs), making strong use of their parallel processing capability. In an…
We describe the effect of optical aberrations on fluorescence fluctuations microscopy (FFM), when focusing through a single living cell. FFM measurements are performed in an aqueous fluorescent solution and prove to be a highly sensitive…
This work introduces the first closed-loop adaptive optics (AO) system capable of optically correcting aberrations in real-time without a guidestar or a wavefront sensor. Nearly 40 years ago, Cederquist et al. demonstrated that asymmetric…