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In fluorescence microscopy, Single Molecule Localization Microscopy (SMLM) techniques aim at localizing with high precision high density fluorescent molecules by stochastically activating and imaging small subsets of blinking emitters.…

Scanning X-ray nanodiffraction microscopy is a powerful technique for spatially resolving nanoscale structural morphologies by diffraction contrast. One of the critical challenges in experimental nanodiffraction data analysis is posed by…

Applied Physics · Physics 2024-06-26 Aileen Luo , Tao Zhou , Martin V. Holt , Andrej Singer , Mathew J. Cherukara

Localization of single fluorescent molecules is key for physicochemical and biophysical measurements such as single-molecule tracking and super-resolution imaging by single-molecule localization microscopy (SMLM). Recently a series of…

Optics · Physics 2021-09-06 Luciano A. Masullo , Lucía F. Lopez , Fernando D. Stefani

Single Molecule Localization Microscopy (SMLM) enables the acquisition of high-resolution images by alternating between activation of a sparse subset of fluorescent molecules present in a sample and localization. In this work, the…

Image and Video Processing · Electrical Eng. & Systems 2018-12-17 Arne Bechensteen , Laure Blanc-Féraud , Gilles Aubert

Three-dimensional particle tracking is an essential tool in studying dynamics under the microscope, namely, fluid dynamics in microfluidic devices, bacteria taxis, cellular trafficking. The 3d position can be determined using 2d imaging…

Computer Vision and Pattern Recognition · Computer Science 2015-09-08 Eldad Afik

Tracking cells in 3D at high speed continues to attract extensive attention for many biomedical applications, such as monitoring immune cell migration and observing tumor metastasis in flowing blood vessels. Here, we propose a deep…

Optics · Physics 2018-05-16 Kan Liu , Hui Qiao , Jiamin Wu , Haoqian Wang , Lu Fang , Qionghai Dai

Beam alignment enables efficient, stable transmission and control of optical energy and information, which critically depend on precise monitoring and regulation of the three-dimensional (3D) relative positioning between fibers. This study…

Optics · Physics 2025-11-27 Lin Xu , Li Pei , Jianshuai Wang , Zhouyi Hu , Tigang Ning

Single molecule localization microscopy (SMLM) permits the visualization of cellular structures an order of magnitude smaller than the diffraction limit of visible light, and an accurate, objective evaluation of the resolution of an SMLM…

Monocular multi-object detection and localization in 3D space has been proven to be a challenging task. The MoNet3D algorithm is a novel and effective framework that can predict the 3D position of each object in a monocular image and draw a…

Computer Vision and Pattern Recognition · Computer Science 2020-06-30 Xichuan Zhou , Yicong Peng , Chunqiao Long , Fengbo Ren , Cong Shi

Single-molecule localization microscopy (SMLM) allows reconstructing biology-relevant structures beyond the diffraction limit by detecting and localizing individual fluorophores -- fluorescent molecules stained onto the observed specimen --…

Computer Vision and Pattern Recognition · Computer Science 2026-03-03 Romain Seailles , Jean-Baptiste Masson , Jean Ponce , Julien Mairal

Neural networks have shown significant potential in solving partial differential equations (PDEs). While deep networks are capable of approximating complex functions, direct one-shot training often faces limitations in both accuracy and…

Numerical Analysis · Mathematics 2025-03-10 Mingxing Weng , Zhiping Mao , Jie Shen

This paper studies 3-D distributed network localization using mixed types of local relative measurements. Each node holds a local coordinate frame without a common orientation and can only measure one type of information (relative position,…

Signal Processing · Electrical Eng. & Systems 2023-12-19 Xu Fang , Xiaolei Li , Lihua Xie

The reduced cost and computational and calibration requirements of monocular cameras make them ideal positioning sensors for mobile robots, albeit at the expense of any meaningful depth measurement. Solutions proposed by some scholars to…

Robotics · Computer Science 2024-04-30 Yi Shen , Hao Liu , Xinxin Liu , Wenjing Zhou , Chang Zhou , Yizhou Chen

Single-molecule localization microscopy (SMLM) surpasses the diffraction limit, achieving subcellular resolution. Traditional SMLM analysis methods often rely on point spread function (PSF) model fitting, limiting the application of complex…

Quantitative Methods · Quantitative Biology 2024-10-04 Tingdan Luo

Super-resolution imaging based on single molecule localization allows accessing nanometric-scale information in biological samples with high precision. However, complete measurements including molecule orientation are still challenging.…

The location of a robot is a key aspect in the field of mobile robotics. This problem is particularly complex when the initial pose of the robot is unknown. In order to find a solution, it is necessary to perform a global localization. In…

Robotics · Computer Science 2025-05-27 Míriam Máximo , Antonio Santo , Arturo Gil , Mónica Ballesta , David Valiente

In this paper, we address the problem of estimating the migration direction of cells based on a single image. A solution to this problem lays the foundation for a variety of applications that were previously not possible. To our knowledge,…

Computer Vision and Pattern Recognition · Computer Science 2025-10-30 Lennart Bruns , Lucas Lamparter , Milos Galic , Xiaoyi Jiang

A long-standing challenge in multiple-particle-tracking is the accurate and precise 3D localization of individual particles at close proximity. One established approach for snapshot 3D imaging is point-spread-function (PSF) engineering, in…

Image and Video Processing · Electrical Eng. & Systems 2020-10-01 Elias Nehme , Boris Ferdman , Lucien E. Weiss , Tal Naor , Daniel Freedman , Tomer Michaeli , Yoav Shechtman

Background fluorescence, especially when it exhibits undesired spatial features, is a primary factor for reduced image quality in optical microscopy. Structured background is particularly detrimental when analyzing single-molecule images…

Optics · Physics 2020-03-25 Leonhard Möckl , Anish R. Roy , Petar N. Petrov , W. E. Moerner

The use of fluorescent molecules to create long sequences of low-density, diffraction-limited images enables highly-precise molecule localization. However, this methodology requires lengthy imaging times, which limits the ability to view…

Image and Video Processing · Electrical Eng. & Systems 2024-03-26 Yair Ben Sahel , Yonina C. Eldar