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Photothermal-Induced Resonance (PTIR) spectroscopy and imaging with infrared light has seen increasing application in molecular spectroscopy of biological samples. The appeal of the technique lies in its capability to provide information…

Quantitative Methods · Quantitative Biology 2019-11-19 Luca Quaroni

Infrared (IR) nanospectroscopy by photothermal induced resonance (PTIR) is a novel experimental technique that combines the nanoscale resolution granted by atomic force microscopy (AFM) and the chemical labelling made possible by IR…

We use photothermal induced resonance (PTIR) imaging and spectroscopy, in resonant and non-resonant mode, to study the cytoplasmic membrane and surface of intact cells. Non-resonant PTIR images apparently provide rich details of the cell…

Applied Physics · Physics 2021-01-27 Luca Quaroni

IR spectroscopy has been widely used for chemical identification and quantitative analysis of reactions occurring in a specific time and space domains by measuring an average signal of the entire system1. Achieving IR measurements with…

Atomic Force Microscopy - Infrared (AFM-IR) has emerged as a useful technique for measuring absorption spectra with spatial resolution better than the optical diffraction limit. The technique relies on the movement of a probe for atomic…

Applied Physics · Physics 2024-01-17 Luca Quaroni

We demonstrate instrumentation and methods to enable fluorescence-detected photothermal infrared (F-PTIR) microscopy, then demonstrate the utility of F-PTIR to characterize the composition within phase-separated domains of model amorphous…

Accurate direct measurements of far-field thermal infrared emission become increasingly important because conventional methods, relying on indirect assessments, such as reflectance/transmittance, are inaccurate or even unfeasible to…

Instrumentation and Detectors · Physics 2023-03-14 Xiu Liu , Hakan Salihoglu , Xiao Luo , Hyeong Seok Yun , Lin Jing , Bowen Yu , Sheng Shen

Fourier-transform infrared spectroscopy (FTIR) has matured into a versatile technique with relevance for environmental monitoring, pharmaceutical research, and food safety applications. However, compared to other spectroscopic methods, it…

Instrumentation and Detectors · Physics 2024-09-04 Jakub Mnich , Johannes Kunsch , Matthias Budden , Thomas Gebert , Marco Schossig , Jarosław Sotor , Łukasz A. Sterczewski

IR spectroscopy such as Fourier transform infrared spectroscopy (FTIR) are widely used for the investigation of structure and the quantitative determination of substances in the fields of chemistry, physics, biology, medicine, and…

Induced transparency is a common but remarkable effect in optics. It occurs when a strong driving field is used to render an otherwise opaque material transparent. The effect is known as electromagnetically induced transparency in atomic…

In this tutorial, we combine the different scientific fields of information theory, thermodynamics, regularization theory and non-destructive imaging, especially for photoacoustic and photothermal imaging. The goal is to get a better…

Applied Physics · Physics 2020-12-02 Peter Burgholzer , Guenther Mayr , Gregor Thummerer , Markus Haltmeier

We discuss the Surface-Plasmon-Resonance (SPR) technique based on Fourier -Transform - InfraRed (FTIR) spectrometry. We explore the potential of the infrared surface plasmon resonance technique for biological studies in aqueous solutions…

Biological Physics · Physics 2010-02-03 M. Golosovsky , V. Lirtsman , V. Yashunsky , D. Davidov , B. Aroeti

We proposed a new approach, which is inspired by the method of super-resolution (SR) structured illumination microscopy (SIM) for overcoming the resolution limit in microscopy due to diffraction of light, for increasing the resolution of…

Medical Physics · Physics 2026-02-04 Youdong Lang , Qingguo Xie , Chien-Min Kao

In this work we present results of the analysis of thermal properties of high-power laser bars obtained by spatially resolved thermoreflectance (TR) spectroscopy. Thermoreflectance is a modulation technique relying on periodic facet…

Materials Science · Physics 2015-05-13 D. Pierscinska , K. Pierscinski , M. Bugajski , J. W. Tomm

Controlling thermal emission is essential for various infrared spectroscopy applications. Metasurfaces can be utilized to control multiple degrees of freedom of thermal emission, enabling the compact thermal emission materials and devices.…

Optics · Physics 2023-11-14 Qiongqiong Chu , Fengyuan Zhang , Ye Zhang , Shining Zhu , Hui Liu

Acoustic-Resolution Photoacoustic Microscopy (AR-PAM) is promising for subcutaneous vascular imaging, but its spatial resolution is constrained by the Point Spread Function (PSF). Traditional deconvolution methods like Richardson-Lucy and…

Computer Vision and Pattern Recognition · Computer Science 2024-10-29 Youshen Xiao , Sheng Liao , Xuanyang Tian , Fan Zhang , Xinlong Dong , Yunhui Jiang , Xiyu Chen , Ruixi Sun , Yuyao Zhang , Fei Gao

Infrared (IR) spectroscopy, a well established tool for chemical analysis of diverse materials, has significant potential in biomedical applications. While the spatial resolution of traditional IR spectroscopy is limited by the wavelength…

Infrared thermography (IRT) and photothermal coherence tomography (PCT) exhibit potential in non-destructive testing and biomedical fields. However, the inevitable heat diffusion significantly affects the sensitivity and resolution of IRT…

Minfrared spectroscopic imaging (MIRSI) is an emerging class of label-free, biochemically quantitative technologies targeting digital histopathology. Conventional histopathology relies on chemical stains that alter tissue color. This…

Image and Video Processing · Electrical Eng. & Systems 2022-07-26 Mahsa Lotfollahi , Nguyen Tran , Sebastian Berisha , Chalapathi Gajjela , Zhu Han , David Mayerich , Rohith Reddy

Fourier transform infrared (FTIR) spectrometers have been the dominant technology in the field of mid-infrared (MIR)spectroscopy for decades. Supercontinuum laser sources operating in the MIR spectral region now offer the potential to…

Instrumentation and Detectors · Physics 2020-04-17 Ivan Zorin , Jakob Kilgus , Kristina Duswald , Bernhard Lendl , Bettina Heise , Markus Brandstetter
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