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We present experimental demonstration of tilt-mirror assisted transmission structured illumination microscopy (tSIM) that offers a large field of view super resolution imaging. An assembly of custom-designed tilt-mirrors are employed as the…

Structured illumination microscopy (SIM) can achieve a $2\times$ resolution enhancement beyond the classical diffraction limit by employing illumination translations with respect to the object. This method has also been successfully…

The blind structured illumination microscopy (SIM) strategy proposed in (Mudry et al., 1992) is fully re-founded in this paper, unveiling the central role of the sparsity of the illumination patterns in the mechanism that drives…

Data Analysis, Statistics and Probability · Physics 2017-04-26 Simon Labouesse , Awoke Negash , Jérôme Idier , Sébastien Bourguignon , Thomas Mangeat , Penghuan Liu , Anne Sentenac , Marc Allain

Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single…

Optics · Physics 2025-06-17 Ruiming Cao , Guanghan Meng , Laura Waller

Digital holographic microscopy (DHM) applied to quantitative phase imaging (QPI) has been successfully demonstrated as a powerful label-free method to analyse the optical properties of cells. Spatially multiplexed interferometric microscopy…

Super-resolution structured illumination microscopy (SR-SIM) is a widely used technique for enhancing the resolution of fluorescence imaging beyond the diffraction limit. Most existing SR-SIM methods rely on Moir\'e effect-based physical…

Optics · Physics 2025-02-27 Junzheng Peng , Jiahao Xian , Xi Lin , Manhong Yao , Shiping Li , Jingang Zhong

Structured illumination microscopy (SIM) has attained high spatiotemporal delineation of subcellular architecture, yet offers limited insight into chemical composition. We develop Chem-SIM, a structured-illumination fluorescence detected…

Structured Illumination Microscopy (SIM) is an imaging technique for achieving both super-resolution (SR) and optical sectioning (OS) in wide-field microscopy. It consists in illuminating the sample with periodic patterns at different…

High-content biological microscopy targets high-resolution imaging across large fields-of-view (FOVs). Recent works have demonstrated that computational imaging can provide efficient solutions for high-content microscopy. Here, we use…

Image and Video Processing · Electrical Eng. & Systems 2019-03-28 Li-Hao Yeh , Shwetadwip Chowdhury , Laura Waller

Structured illumination microscopy (SIM) reconstructs a super-resolved image from multiple raw images captured with different illumination patterns; hence, acquisition speed is limited, making it unsuitable for dynamic scenes. We propose a…

Optics · Physics 2025-06-17 Ruiming Cao , Fanglin Linda Liu , Li-Hao Yeh , Laura Waller

Nonlinear structured illumination microscopy (nSIM) is an effective approach for super-resolution wide-field fluorescence microscopy with a theoretically unlimited resolution. In nSIM, carefully designed, highly-contrasted illumination…

In this communication, a fast reconstruction algorithm is proposed for fluorescence \textit{blind} structured illumination microscopy (SIM) under the sample positivity constraint. This new algorithm is by far simpler and faster than…

Data Analysis, Statistics and Probability · Physics 2016-11-18 S. Labouesse , M. Allain , J. Idier , S. Bourguignon , A. Negash , P. Liu , A. Sentenac

Super-resolution fluorescence microscopy is of great interest in life science studies for visualizing subcellular structures at the nanometer scale. Among various kinds of super-resolution approaches, image scanning microscopy (ISM) offers…

Confocal microscopy, a critical advancement in optical imaging, is widely applied because of its excellent anti-noise ability. However, it has low imaging efficiency and can cause phototoxicity. Optical-sectioning structured illumination…

Optics · Physics 2024-05-27 Weishuai Zhou , Manhong Yao , Xi Lin , Quan Yu , Junzheng Peng , Jingang Zhong

In this paper, a compact and low-cost structured illumination microscope (SIM) based on a 2X2 fiber coupler is presented. Fringe illumination is achieved by placing two output fiber tips at a conjugate Fourier plane of the sample plane as…

Instrumentation and Detectors · Physics 2018-12-19 Shiming Hu , Lei Liu , Yizheng Huang , Wenwen Liu , Qingquan Wei , Manqing Tan , Yude Yu

We propose to enhance the performance of localized plasmon structured illumination microscopy (LP-SIM) via intensity correlations. LP-SIM uses sub-wavelength illumination patterns to encode high spatial frequency information. It can enhance…

We describe a two-beam interference structured illumination fluorescence microscope. The novelty of the presented system lies in its simplicity. A programmable electro-optical spatial light modulator in an intermediate image plane enables…

Structured illumination microscopy (SIM) is an optical super-resolution technique that enables live-cell imaging beyond the diffraction limit. Reconstruction of SIM data is prone to artefacts, which becomes problematic when imaging highly…

Image and Video Processing · Electrical Eng. & Systems 2022-03-02 Charles N. Christensen , Meng Lu , Edward N. Ward , Pietro Lio , Clemens F. Kaminski

Modern optical microscopy methods have been advanced to provide super resolution at high imaging speed, but not chirality discriminative. We recently proposed chiral structured-illumination microscopy (SIM) method to image chiral…

Optics · Physics 2021-03-11 Jiwei Zhang , Shiang-Yu Huang , Ankit Kumar Singh , Jer-Shing Huang

We present a multimodal approach for measuring the three-dimensional (3D) refractive index (RI) and fluorescence distributions of live cells by combining optical diffraction tomography (ODT) and 3D structured illumination microscopy (SIM).…

Optics · Physics 2018-01-04 Seungwoo Shin , Doyeon Kim , Kyoohyun Kim , Yongkeun Park