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Liquid liquid phase separation (LLPS) mediated by pi-cation bonds between tyrosine and arginine residues are of biological importance. To understand the interactions between proteins in the condensed phase in close analogy to complex…

Biological Physics · Physics 2020-08-19 Aditya N. Singh , Arun Yethiraj

Liquid-liquid phase separation (LLPS) of protein solutions is governed by highly complex protein-protein interactions. Nevertheless, it has been suggested that based on the extended law of corresponding states (ELCS), as proposed for…

Soft Condensed Matter · Physics 2023-02-08 Jan Hansen , Stefan U. Egelhaaf , Florian Platten

The ion-activated patchy particle model is an important theoretical framework to investigate the phase behaviour of globular proteins in the presence of multivalent ions. In this work, we study and highlight the influence of patch…

Soft Condensed Matter · Physics 2025-11-13 Furio Surfaro , Peixuan Liang , Hadra Banks , Fajun Zhang , Frank Schreiber , Martin Oettel

Globular proteins as well as recently synthesized colloids engineered with differently charged surface regions have in common a reduced bonding valence and a complex interaction pattern dominated by like-charge attraction and…

Soft Condensed Matter · Physics 2024-02-02 Daniele Notarmuzi , Emanuela Bianchi

Liquid-liquid phase separation (LLPS) is currently of great interest in cell biology. LLPS is an example of what is called an emergent phenomenon -- an idea that comes from condensed-matter physics. Emergent phenomena have the…

Cell Behavior · Quantitative Biology 2021-03-16 Richard P Sear

Microscopic dynamics of complex fluids in the early stage of spinodal decomposition (SD) is strongly intertwined with the kinetics of structural evolution, which makes a quantitative characterization challenging. In this work, we use x-ray…

Proteins and nucleic acids can spontaneously self-assemble into membraneless droplet-like compartments, both in vitro and in vivo. A key component of these droplets are multi-valent proteins that possess several adhesive domains with…

Biomolecules · Quantitative Biology 2020-01-10 Srivastav Ranganathan , Eugene Shakhnovich

The phase behavior of membrane proteins stems from a complex synergy with the amphiphilic molecules required for their solubilization. We show that ionization of a pH-sensitive surfactant, LDAO, bound to a bacterial photosynthetic protein,…

Soft Condensed Matter · Physics 2009-11-07 R. Piazza , M. Pierno , E. Vignati , G. Venturoli , F. Francia , A. Mallardi , G. Palazzo

We investigate theoretically the behavior of proteins as well as other large macromolecules which are incorporated into amphiphilic monolayers at the air-water interface. We assume the monolayer to be in the coexistence region of the…

Soft Condensed Matter · Physics 2007-05-23 R. R. Netz , D. Andelman , H. Orland

Employing X-ray photon correlation spectroscopy we measure the kinetics and dynamics of a pressure-induced liquid-liquid phase separation (LLPS) in a water-lysozyme solution. Scattering invariants and kinetic information provide evidence…

Soft Condensed Matter · Physics 2021-07-13 M. Moron , A. Al-Masoodi , C. Lovato , M. Reiser , L. Randolph , G. Surmeier , J. Bolle , F. Westermeier , M. Sprung , R. Winter , M. Paulus , C. Gutt

Demixing of binary liquids is a ubiquitous transition, which is explained using a well-established thermodynamic formalism that requires equality of intensive thermodynamics parameters across the phase boundaries. Demixing transitions also…

The dilute fluid phase of model globular proteins is studied. The model possesses a fluid-fluid transition buried within the fluid-crystal coexistence region, as do some globular proteins. If this fluid-fluid transition is not buried deep…

Soft Condensed Matter · Physics 2007-05-23 Richard P. Sear

The rapid adhesion of motile bacteria from dilute suspensions poses a fundamental non-equilibrium problem: hydrodynamic interactions bias bacterial motion near surfaces without generating stable confinement, while electrostatic interactions…

Soft Condensed Matter · Physics 2026-01-13 Dixi Yang , Anheng Wang , Jia Huang , Xiaofeng Zhuo , Chunming Wang , Hajime Tanaka , Jiaxing Yuan

Liquid liquid phase separation (LLPS) of proteins is an intracellular process that is widely used by cells for many purposes. In living cells (in vivo), LLPS occurs in complex and crowded environments. Amino acids (AAs) are vital components…

We studied the crystallization of a globular protein, lysozyme, in the cubic phase of the lipid monoolein. The solubility of lysozyme in salt solution decreased by a factor of $\sim 4$ when confined in cubic phase. Calculations and Monte…

Soft Condensed Matter · Physics 2007-05-23 S. Tanaka , S. U. Egelhaaf , W. C. K. Poon

Biomolecular condensates play essential roles in cellular processes, and recent efforts have focused on understanding their assembly and rational design principles. In this study, we have employed an explicit-solvent minimal statistical…

Soft Condensed Matter · Physics 2026-05-26 Siddhartha Roy , Rakesh S. Singh

Active liquid-liquid phase separation (LLPS) in a confining environment is believed to play an important role in cell biology. Recently, it was shown that when active noise at the microscopic level is included in the classical theory of…

Soft Condensed Matter · Physics 2024-08-16 Chen Lin , Robijn Bruinsma

We investigate by time-resolved Synchrotron ultra-small X-ray scattering the dynamics of liquid-liquid phase-separation (LLPS) of gluten protein suspensions following a temperature quench. Samples at a fixed concentration (237 mg/ml) but…

Soft Condensed Matter · Physics 2019-08-27 A. Banc , J. Pincemaille , S. Costanzo , E. Chauveau , M. s. Appavou , M. H. Morel , P. Menut , L. Ramos

Achieving a coherent understanding of the many thermodynamic and dynamic anomalies of water is among the most important unsolved puzzles in physics, chemistry, and biology. One hypothesized explanation imagines the existence of a line of…

Statistical Mechanics · Physics 2012-06-01 Tobias Kesselring , Giancarlo Franzese , Sergey Buldyrev , Hans Herrmann , H. Eugene Stanley

A system of identical particles interacting through an isotropic potential that allows for two preferred interparticle distances is numerically studied. When the parameters of the interaction potential are adequately chosen, the system…

Condensed Matter · Physics 2009-10-31 E. A. Jagla
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